PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility

Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity u...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Oleksandr Gryshkov, Fedaa AL Halabi, Antonia Isabel Kuhn, Sara Leal-Marin, Lena Julie Freund, Maria Förthmann, Nils Meier, Sven-Alexander Barker, Kirsten Haastert-Talini, Birgit Glasmacher
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyvinylidene fluoride polyvinylidene fluoride-co-trifluoroethylene electrospinning scaffold piezoelectric module dynamic impact machine Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/22cdd008d33041c094e8a7a372f64d2e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:22cdd008d33041c094e8a7a372f64d2e
record_format	dspace
spelling	oai:doaj.org-article:22cdd008d33041c094e8a7a372f64d2e2021-11-11T16:50:47ZPVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility10.3390/ijms2221113731422-00671661-6596https://doaj.org/article/22cdd008d33041c094e8a7a372f64d2e2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11373https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant <i>d</i><sub>33</sub>, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair.Oleksandr GryshkovFedaa AL HalabiAntonia Isabel KuhnSara Leal-MarinLena Julie FreundMaria FörthmannNils MeierSven-Alexander BarkerKirsten Haastert-TaliniBirgit GlasmacherMDPI AGarticlepolyvinylidene fluoridepolyvinylidene fluoride-co-trifluoroethyleneelectrospinningscaffoldpiezoelectric moduledynamic impact machineBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11373, p 11373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyvinylidene fluoride polyvinylidene fluoride-co-trifluoroethylene electrospinning scaffold piezoelectric module dynamic impact machine Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	polyvinylidene fluoride polyvinylidene fluoride-co-trifluoroethylene electrospinning scaffold piezoelectric module dynamic impact machine Biology (General) QH301-705.5 Chemistry QD1-999 Oleksandr Gryshkov Fedaa AL Halabi Antonia Isabel Kuhn Sara Leal-Marin Lena Julie Freund Maria Förthmann Nils Meier Sven-Alexander Barker Kirsten Haastert-Talini Birgit Glasmacher PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
description	Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant <i>d</i><sub>33</sub>, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair.
format	article
author	Oleksandr Gryshkov Fedaa AL Halabi Antonia Isabel Kuhn Sara Leal-Marin Lena Julie Freund Maria Förthmann Nils Meier Sven-Alexander Barker Kirsten Haastert-Talini Birgit Glasmacher
author_facet	Oleksandr Gryshkov Fedaa AL Halabi Antonia Isabel Kuhn Sara Leal-Marin Lena Julie Freund Maria Förthmann Nils Meier Sven-Alexander Barker Kirsten Haastert-Talini Birgit Glasmacher
author_sort	Oleksandr Gryshkov
title	PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
title_short	PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
title_full	PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
title_fullStr	PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
title_full_unstemmed	PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility
title_sort	pvdf and p(vdf-trfe) electrospun scaffolds for nerve graft engineering: a comparative study on piezoelectric and structural properties, and in vitro biocompatibility
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/22cdd008d33041c094e8a7a372f64d2e
work_keys_str_mv	AT oleksandrgryshkov pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT fedaaalhalabi pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT antoniaisabelkuhn pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT saralealmarin pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT lenajuliefreund pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT mariaforthmann pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT nilsmeier pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT svenalexanderbarker pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT kirstenhaasterttalini pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility AT birgitglasmacher pvdfandpvdftrfeelectrospunscaffoldsfornervegraftengineeringacomparativestudyonpiezoelectricandstructuralpropertiesandinvitrobiocompatibility
_version_	1718432227249881088

PVDF and P(VDF-TrFE) Electrospun Scaffolds for Nerve Graft Engineering: A Comparative Study on Piezoelectric and Structural Properties, and In Vitro Biocompatibility

Ejemplares similares