Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation

The protein film adsorbed on joint prosthesis material affects tribological property. It is expected that optimum protein film structure could improve friction and wear of joint prosthesis materials. Therefore, it is important to understand and to clarify the effect of protein film on tribological p...

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Autores principales:	Kazuhiro Nakashima, Yoshinori Sawae, Teruo Murakami, Stefano Mischler
Formato:	article
Lenguaje:	EN
Publicado:	Japanese Society of Tribologists 2015
Materias:	boundary lubrication natural synovial fluid constituents protein adsorption electrochemical method bovine serum albumin ultra-high molecular weight polyethylene cocrmo alloy joint prosthesis material.tribology design maintenance trouble case study bearing seal Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/22dac9008ef2415588bb667a4b97df9a
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id	oai:doaj.org-article:22dac9008ef2415588bb667a4b97df9a
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spelling	oai:doaj.org-article:22dac9008ef2415588bb667a4b97df9a2021-11-05T09:22:37ZBehavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation1881-219810.2474/trol.10.183https://doaj.org/article/22dac9008ef2415588bb667a4b97df9a2015-04-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/trol/10/2/10_183/_pdf/-char/enhttps://doaj.org/toc/1881-2198The protein film adsorbed on joint prosthesis material affects tribological property. It is expected that optimum protein film structure could improve friction and wear of joint prosthesis materials. Therefore, it is important to understand and to clarify the effect of protein film on tribological property. In the rubbing combination of ultra-high molecular weight polyethylene (UHMWPE) and CoCrMo alloy, the adsorption behavior of bovine serum albumin (BSA) and its effect on frictional property during friction were investigated by using electrochemical method. Friction force and electric potential were measured under open circuit potential (OCP) condition, but friction force and electric current were measured under –0.2 V applied potential condition. Under OCP condition, adsorbed BSA was peeled off at first rubbing, after that BSA re-adsorbed on surface under shear force condition. BSA adsorbed film reconstructed by shear force was sustained during rubbing. It is considered that the conformation of adsorbed BSA under shear stress was changed from that of static adsorption, because readsorbed BSA molecule did not show exfoliation from metal surface, which means that adsorption strength was increased from static adsorption. Under applied potential condition where wear performance of BSA film is expected to be evaluated, BSA showed the desorption at first rubbing, and gradual decrease in desorption amount. Therefore, it is confirmed that BSA film on metal surface under rubbing condition is reconstructed during rubbing. The adsorbed film formed during rubbing is stable and has an optimally-adapted structure for shear force and can sustain its property during rubbing.Kazuhiro NakashimaYoshinori SawaeTeruo MurakamiStefano MischlerJapanese Society of Tribologistsarticleboundary lubricationnatural synovial fluid constituentsprotein adsorptionelectrochemical methodbovine serum albuminultra-high molecular weight polyethylenecocrmo alloyjoint prosthesis material.tribologydesignmaintenancetroublecase studybearingsealPhysicsQC1-999Engineering (General). Civil engineering (General)TA1-2040Mechanical engineering and machineryTJ1-1570ChemistryQD1-999ENTribology Online, Vol 10, Iss 2, Pp 183-189 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	boundary lubrication natural synovial fluid constituents protein adsorption electrochemical method bovine serum albumin ultra-high molecular weight polyethylene cocrmo alloy joint prosthesis material.tribology design maintenance trouble case study bearing seal Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
spellingShingle	boundary lubrication natural synovial fluid constituents protein adsorption electrochemical method bovine serum albumin ultra-high molecular weight polyethylene cocrmo alloy joint prosthesis material.tribology design maintenance trouble case study bearing seal Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999 Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami Stefano Mischler Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
description	The protein film adsorbed on joint prosthesis material affects tribological property. It is expected that optimum protein film structure could improve friction and wear of joint prosthesis materials. Therefore, it is important to understand and to clarify the effect of protein film on tribological property. In the rubbing combination of ultra-high molecular weight polyethylene (UHMWPE) and CoCrMo alloy, the adsorption behavior of bovine serum albumin (BSA) and its effect on frictional property during friction were investigated by using electrochemical method. Friction force and electric potential were measured under open circuit potential (OCP) condition, but friction force and electric current were measured under –0.2 V applied potential condition. Under OCP condition, adsorbed BSA was peeled off at first rubbing, after that BSA re-adsorbed on surface under shear force condition. BSA adsorbed film reconstructed by shear force was sustained during rubbing. It is considered that the conformation of adsorbed BSA under shear stress was changed from that of static adsorption, because readsorbed BSA molecule did not show exfoliation from metal surface, which means that adsorption strength was increased from static adsorption. Under applied potential condition where wear performance of BSA film is expected to be evaluated, BSA showed the desorption at first rubbing, and gradual decrease in desorption amount. Therefore, it is confirmed that BSA film on metal surface under rubbing condition is reconstructed during rubbing. The adsorbed film formed during rubbing is stable and has an optimally-adapted structure for shear force and can sustain its property during rubbing.
format	article
author	Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami Stefano Mischler
author_facet	Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami Stefano Mischler
author_sort	Kazuhiro Nakashima
title	Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
title_short	Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
title_full	Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
title_fullStr	Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
title_full_unstemmed	Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation
title_sort	behavior of adsorbed albumin film on cocrmo alloy under in-situ observation
publisher	Japanese Society of Tribologists
publishDate	2015
url	https://doaj.org/article/22dac9008ef2415588bb667a4b97df9a
work_keys_str_mv	AT kazuhironakashima behaviorofadsorbedalbuminfilmoncocrmoalloyunderinsituobservation AT yoshinorisawae behaviorofadsorbedalbuminfilmoncocrmoalloyunderinsituobservation AT teruomurakami behaviorofadsorbedalbuminfilmoncocrmoalloyunderinsituobservation AT stefanomischler behaviorofadsorbedalbuminfilmoncocrmoalloyunderinsituobservation
_version_	1718444396664324096

Behavior of Adsorbed Albumin Film on CoCrMo Alloy under In-situ Observation

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