A new and improved genome sequence of Cannabis sativa

Cannabis is a diploid species (2n = 20), the estimated haploid genome sizes of the female and male plants using flow cytometry are 818 and 843 Mb respectively. Although the genome of Cannabis has been sequenced (from hemp, wild and high-THC strains), all assemblies have significant gaps...

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Autores principales:	Shivraj Braich, Rebecca C. Baillie, German C. Spangenberg, Noel O. I. Cogan
Formato:	article
Lenguaje:	EN
Publicado:	GigaScience Press 2020
Materias:	Electronic computers. Computer science QA75.5-76.95
Acceso en línea:	https://doaj.org/article/22db8cee360e447b8e99ffac831b640a
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spelling	oai:doaj.org-article:22db8cee360e447b8e99ffac831b640a2021-12-02T15:56:04ZA new and improved genome sequence of Cannabis sativa10.46471/gigabyte.102709-4715https://doaj.org/article/22db8cee360e447b8e99ffac831b640a2020-12-01T00:00:00Zhttps://gigabytejournal.com/articles/10https://doaj.org/toc/2709-4715 Cannabis is a diploid species (2n = 20), the estimated haploid genome sizes of the female and male plants using flow cytometry are 818 and 843 Mb respectively. Although the genome of Cannabis has been sequenced (from hemp, wild and high-THC strains), all assemblies have significant gaps. In addition, there are inconsistencies in the chromosome numbering which limits their use. A new comprehensive draft genome sequence assembly (∼900 Mb) has been generated from the medicinal cannabis strain Cannbio-2, that produces a balanced ratio of cannabidiol and delta-9-tetrahydrocannabinol using long-read sequencing. The assembly was subsequently analysed for completeness by ordering the contigs into chromosome-scale pseudomolecules using a reference genome assembly approach, annotated and compared to other existing reference genome assemblies. The Cannbio-2 genome sequence assembly was found to be the most complete genome sequence available based on nucleotides assembled and BUSCO evaluation in Cannabis sativa with a comprehensive genome annotation. The new draft genome sequence is an advancement in Cannabis genomics permitting pan-genome analysis, genomic selection as well as genome editing. Shivraj BraichRebecca C. BaillieGerman C. SpangenbergNoel O. I. CoganGigaScience PressarticleElectronic computers. Computer scienceQA75.5-76.95ENGigaByte (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronic computers. Computer science QA75.5-76.95
spellingShingle	Electronic computers. Computer science QA75.5-76.95 Shivraj Braich Rebecca C. Baillie German C. Spangenberg Noel O. I. Cogan A new and improved genome sequence of Cannabis sativa
description	Cannabis is a diploid species (2n = 20), the estimated haploid genome sizes of the female and male plants using flow cytometry are 818 and 843 Mb respectively. Although the genome of Cannabis has been sequenced (from hemp, wild and high-THC strains), all assemblies have significant gaps. In addition, there are inconsistencies in the chromosome numbering which limits their use. A new comprehensive draft genome sequence assembly (∼900 Mb) has been generated from the medicinal cannabis strain Cannbio-2, that produces a balanced ratio of cannabidiol and delta-9-tetrahydrocannabinol using long-read sequencing. The assembly was subsequently analysed for completeness by ordering the contigs into chromosome-scale pseudomolecules using a reference genome assembly approach, annotated and compared to other existing reference genome assemblies. The Cannbio-2 genome sequence assembly was found to be the most complete genome sequence available based on nucleotides assembled and BUSCO evaluation in Cannabis sativa with a comprehensive genome annotation. The new draft genome sequence is an advancement in Cannabis genomics permitting pan-genome analysis, genomic selection as well as genome editing.
format	article
author	Shivraj Braich Rebecca C. Baillie German C. Spangenberg Noel O. I. Cogan
author_facet	Shivraj Braich Rebecca C. Baillie German C. Spangenberg Noel O. I. Cogan
author_sort	Shivraj Braich
title	A new and improved genome sequence of Cannabis sativa
title_short	A new and improved genome sequence of Cannabis sativa
title_full	A new and improved genome sequence of Cannabis sativa
title_fullStr	A new and improved genome sequence of Cannabis sativa
title_full_unstemmed	A new and improved genome sequence of Cannabis sativa
title_sort	new and improved genome sequence of cannabis sativa
publisher	GigaScience Press
publishDate	2020
url	https://doaj.org/article/22db8cee360e447b8e99ffac831b640a
work_keys_str_mv	AT shivrajbraich anewandimprovedgenomesequenceofcannabissativa AT rebeccacbaillie anewandimprovedgenomesequenceofcannabissativa AT germancspangenberg anewandimprovedgenomesequenceofcannabissativa AT noeloicogan anewandimprovedgenomesequenceofcannabissativa AT shivrajbraich newandimprovedgenomesequenceofcannabissativa AT rebeccacbaillie newandimprovedgenomesequenceofcannabissativa AT germancspangenberg newandimprovedgenomesequenceofcannabissativa AT noeloicogan newandimprovedgenomesequenceofcannabissativa
_version_	1718385379159048192

A new and improved genome sequence of Cannabis sativa

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