How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?

Begomoviruses are transmitted by whitefly (Bemisia tabaci Gennadius, Hemiptera: Aleyrodidae) in a persistent-circulative way. Once B. tabaci becomes viruliferous, it remains so throughout its life span. Not much is known about the copies of begomoviruses ingested and/or released by B. tabaci during...

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Autores principales:	Buddhadeb Roy, Prosenjit Chakraborty, Amalendu Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/22e38d87edfe489b995fc8232fe0ac8d
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spelling	oai:doaj.org-article:22e38d87edfe489b995fc8232fe0ac8d2021-12-02T20:13:30ZHow many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?1932-620310.1371/journal.pone.0258933https://doaj.org/article/22e38d87edfe489b995fc8232fe0ac8d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258933https://doaj.org/toc/1932-6203Begomoviruses are transmitted by whitefly (Bemisia tabaci Gennadius, Hemiptera: Aleyrodidae) in a persistent-circulative way. Once B. tabaci becomes viruliferous, it remains so throughout its life span. Not much is known about the copies of begomoviruses ingested and/or released by B. tabaci during the process of feeding. The present study reports the absolute quantification of two different begomoviruses viz. tomato leaf curl New Delhi virus (ToLCNDV, bipartite) and chilli leaf curl virus (ChiLCV, monopartite) at different exposure of active acquisition and inoculation feeding using a detached leaf assay. A million copies of both the begomoviruses were acquired by a single B. tabaci with only 5 min of active feeding and virus copy number increased in a logarithmic model with feeding exposure. Whereas, a single B. tabaci could inoculate 8.21E+09 and 4.19E+11 copies of ToLCNDV and ChiLCV, respectively in detached leaves by 5 min of active feeding. Virus copies in inoculated leaves increased with an increase in feeding duration. Comparative dynamics of these two begomoviruses indicated that B. tabaci adult acquired around 14-fold higher copies of ChiLCV than ToLCNDV 24 hrs post feeding. Whereas, the rate of inoculation of ToLCNDV by individual B. tabaci was significantly higher than ChiLCV. The study provides a better understanding of begomovirus acquisition and inoculation dynamics by individual B. tabaci and would facilitate research on virus-vector epidemiology and screening host resistance.Buddhadeb RoyProsenjit ChakrabortyAmalendu GhoshPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258933 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Buddhadeb Roy Prosenjit Chakraborty Amalendu Ghosh How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
description	Begomoviruses are transmitted by whitefly (Bemisia tabaci Gennadius, Hemiptera: Aleyrodidae) in a persistent-circulative way. Once B. tabaci becomes viruliferous, it remains so throughout its life span. Not much is known about the copies of begomoviruses ingested and/or released by B. tabaci during the process of feeding. The present study reports the absolute quantification of two different begomoviruses viz. tomato leaf curl New Delhi virus (ToLCNDV, bipartite) and chilli leaf curl virus (ChiLCV, monopartite) at different exposure of active acquisition and inoculation feeding using a detached leaf assay. A million copies of both the begomoviruses were acquired by a single B. tabaci with only 5 min of active feeding and virus copy number increased in a logarithmic model with feeding exposure. Whereas, a single B. tabaci could inoculate 8.21E+09 and 4.19E+11 copies of ToLCNDV and ChiLCV, respectively in detached leaves by 5 min of active feeding. Virus copies in inoculated leaves increased with an increase in feeding duration. Comparative dynamics of these two begomoviruses indicated that B. tabaci adult acquired around 14-fold higher copies of ChiLCV than ToLCNDV 24 hrs post feeding. Whereas, the rate of inoculation of ToLCNDV by individual B. tabaci was significantly higher than ChiLCV. The study provides a better understanding of begomovirus acquisition and inoculation dynamics by individual B. tabaci and would facilitate research on virus-vector epidemiology and screening host resistance.
format	article
author	Buddhadeb Roy Prosenjit Chakraborty Amalendu Ghosh
author_facet	Buddhadeb Roy Prosenjit Chakraborty Amalendu Ghosh
author_sort	Buddhadeb Roy
title	How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
title_short	How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
title_full	How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
title_fullStr	How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
title_full_unstemmed	How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?
title_sort	how many begomovirus copies are acquired and inoculated by its vector, whitefly (bemisia tabaci) during feeding?
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/22e38d87edfe489b995fc8232fe0ac8d
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How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?

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