Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue

Parth S Shah,1,2 Shiva Murarka,1 Anupam Joshi,3 Bhavna Mehta,3 Vipal Parmar,3 Nidhi Shah,1,4 Khushbu Patel,1 Jacob Sands5 1Department of Molecular Genetics, Supratech Micropath Laboratory and Research Institute, Ahmedabad, India; 2Department of Medicine, Lahey Hospital and Medical Center, Burlington...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shah PS, Murarka S, Joshi A, Mehta B, Parmar V, Shah N, Patel K, Sands J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	HER2 digital PCR IHC FISH breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/22ea6f1b18c54ae3992dd0ea3c0c92ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:22ea6f1b18c54ae3992dd0ea3c0c92ec
record_format	dspace
spelling	oai:doaj.org-article:22ea6f1b18c54ae3992dd0ea3c0c92ec2021-12-02T00:42:01ZSingle-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue1179-1314https://doaj.org/article/22ea6f1b18c54ae3992dd0ea3c0c92ec2018-07-01T00:00:00Zhttps://www.dovepress.com/single-day-her2neu-amplification-assessment-using-chip-based-digital-p-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Parth S Shah,1,2 Shiva Murarka,1 Anupam Joshi,3 Bhavna Mehta,3 Vipal Parmar,3 Nidhi Shah,1,4 Khushbu Patel,1 Jacob Sands5 1Department of Molecular Genetics, Supratech Micropath Laboratory and Research Institute, Ahmedabad, India; 2Department of Medicine, Lahey Hospital and Medical Center, Burlington, MA, USA; 3Department of Histopathology, Supratech Micropath Laboratory and Research Institute, Ahmedabad, India; 4Department of Pediatrics, Nassau University Medical Center, East Meadow, NY, USA; 5Department of Hematology and Oncology, Lahey Hospital and Medical Center, Burlington, MA, USA Introduction: Human epidermal growth factor receptor 2 (HER2) amplification is present in almost 15%–20% of breast cancer tumors, making it an important parameter for testing. The present study was designed to evaluate a chip-based digital PCR (dPCR) system for assessing HER2 amplification from formalin-fixed paraffin-embedded breast carcinoma tissue and to compare this system with immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Materials and methods: A total of 84 breast carcinoma tissue samples were analyzed by IHC, FISH, and chip-based dPCR in a blinded manner. Results: All nine IHC-positive and 35 IHC-negative samples had equivalent results with dPCR, taking an amplification ratio threshold of 1.8 as a positive result. Of the 40 IHC equivocal samples, 10 were assessed as positive, 27 as negative, and three as equivocal by dPCR. Conclusion: These results demonstrate that chip-based dPCR is suitable for HER2 amplification detection in formalin-fixed paraffin-embedded samples in a clinical setting, providing the advantages of superior turnaround time, cost-effectiveness, and increased precision with absolute quantification compared with conventional tests such as FISH and IHC. This methodology was especially beneficial in tissue samples with low DNA concentration. Keywords: HER2, digital PCR, IHC, FISH, breast cancerShah PSMurarka SJoshi AMehta BParmar VShah NPatel KSands JDove Medical PressarticleHER2digital PCRIHCFISHbreast cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol Volume 10, Pp 121-129 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	HER2 digital PCR IHC FISH breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	HER2 digital PCR IHC FISH breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Shah PS Murarka S Joshi A Mehta B Parmar V Shah N Patel K Sands J Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
description	Parth S Shah,1,2 Shiva Murarka,1 Anupam Joshi,3 Bhavna Mehta,3 Vipal Parmar,3 Nidhi Shah,1,4 Khushbu Patel,1 Jacob Sands5 1Department of Molecular Genetics, Supratech Micropath Laboratory and Research Institute, Ahmedabad, India; 2Department of Medicine, Lahey Hospital and Medical Center, Burlington, MA, USA; 3Department of Histopathology, Supratech Micropath Laboratory and Research Institute, Ahmedabad, India; 4Department of Pediatrics, Nassau University Medical Center, East Meadow, NY, USA; 5Department of Hematology and Oncology, Lahey Hospital and Medical Center, Burlington, MA, USA Introduction: Human epidermal growth factor receptor 2 (HER2) amplification is present in almost 15%–20% of breast cancer tumors, making it an important parameter for testing. The present study was designed to evaluate a chip-based digital PCR (dPCR) system for assessing HER2 amplification from formalin-fixed paraffin-embedded breast carcinoma tissue and to compare this system with immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Materials and methods: A total of 84 breast carcinoma tissue samples were analyzed by IHC, FISH, and chip-based dPCR in a blinded manner. Results: All nine IHC-positive and 35 IHC-negative samples had equivalent results with dPCR, taking an amplification ratio threshold of 1.8 as a positive result. Of the 40 IHC equivocal samples, 10 were assessed as positive, 27 as negative, and three as equivocal by dPCR. Conclusion: These results demonstrate that chip-based dPCR is suitable for HER2 amplification detection in formalin-fixed paraffin-embedded samples in a clinical setting, providing the advantages of superior turnaround time, cost-effectiveness, and increased precision with absolute quantification compared with conventional tests such as FISH and IHC. This methodology was especially beneficial in tissue samples with low DNA concentration. Keywords: HER2, digital PCR, IHC, FISH, breast cancer
format	article
author	Shah PS Murarka S Joshi A Mehta B Parmar V Shah N Patel K Sands J
author_facet	Shah PS Murarka S Joshi A Mehta B Parmar V Shah N Patel K Sands J
author_sort	Shah PS
title	Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
title_short	Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
title_full	Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
title_fullStr	Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
title_full_unstemmed	Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
title_sort	single-day her2neu amplification assessment using chip-based digital pcr in formalin-fixed paraffin-embedded breast carcinoma tissue
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/22ea6f1b18c54ae3992dd0ea3c0c92ec
work_keys_str_mv	AT shahps singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT murarkas singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT joshia singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT mehtab singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT parmarv singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT shahn singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT patelk singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue AT sandsj singledayher2neuamplificationassessmentusingchipbaseddigitalpcrinformalinfixedparaffinembeddedbreastcarcinomatissue
_version_	1718403480306057216

Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue

Ejemplares similares