A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis

Understanding bacterial interactions and assembly in complex microbial communities using 16S rRNA sequencing normally requires a large experimental load. However, the current DNA extraction methods, including cell disruption and genomic DNA purification, are normally biased, costly, time-consuming,...

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Autores principales:	Fangchao Song, Jennifer V. Kuehl, Arjun Chandran, Adam P. Arkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/23059a50abdc456da87dfeb7aa0b0378
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spelling	oai:doaj.org-article:23059a50abdc456da87dfeb7aa0b03782021-12-02T19:44:32ZA Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis2379-507710.1128/mSystems.00224-21https://doaj.org/article/23059a50abdc456da87dfeb7aa0b03782021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00224-21https://doaj.org/toc/2379-5077 Understanding bacterial interactions and assembly in complex microbial communities using 16S rRNA sequencing normally requires a large experimental load. However, the current DNA extraction methods, including cell disruption and genomic DNA purification, are normally biased, costly, time-consuming, labor-intensive, and not amenable to miniaturization by droplets or 1,536-well plates due to the significant DNA loss during the purification step for tiny-volume and low-cell-density samples.Fangchao SongJennifer V. KuehlArjun ChandranAdam P. ArkinAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Fangchao Song Jennifer V. Kuehl Arjun Chandran Adam P. Arkin A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
description	Understanding bacterial interactions and assembly in complex microbial communities using 16S rRNA sequencing normally requires a large experimental load. However, the current DNA extraction methods, including cell disruption and genomic DNA purification, are normally biased, costly, time-consuming, labor-intensive, and not amenable to miniaturization by droplets or 1,536-well plates due to the significant DNA loss during the purification step for tiny-volume and low-cell-density samples.
format	article
author	Fangchao Song Jennifer V. Kuehl Arjun Chandran Adam P. Arkin
author_facet	Fangchao Song Jennifer V. Kuehl Arjun Chandran Adam P. Arkin
author_sort	Fangchao Song
title	A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
title_short	A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
title_full	A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
title_fullStr	A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
title_full_unstemmed	A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
title_sort	simple, cost-effective, and automation-friendly direct pcr approach for bacterial community analysis
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/23059a50abdc456da87dfeb7aa0b0378
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A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis

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