A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift

Abstract In mammals, the central circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and it orchestrates peripheral clocks in the whole body to organize physiological and behavioral rhythms. Light-induced phase-shift of the SCN clock enables synchronization of the cir...

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Autores principales:	Hideki Terajima, Hikari Yoshitane, Tomoko Yoshikawa, Yasufumi Shigeyoshi, Yoshitaka Fukada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Pituitary Adenylyl Cyclase-activating Polypeptide (PACAP) ADAR2 Knockout Mice Bioluminescence Rhythm Light-induced Phase Advances mRNA Rhythm Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2306fa7987464a5eb119a4d7b722dfab
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spelling	oai:doaj.org-article:2306fa7987464a5eb119a4d7b722dfab2021-12-02T11:41:23ZA-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift10.1038/s41598-018-33114-62045-2322https://doaj.org/article/2306fa7987464a5eb119a4d7b722dfab2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33114-6https://doaj.org/toc/2045-2322Abstract In mammals, the central circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and it orchestrates peripheral clocks in the whole body to organize physiological and behavioral rhythms. Light-induced phase-shift of the SCN clock enables synchronization of the circadian clock system with 24-h environmental light/dark cycle. We previously found that adenosine deaminase acting on RNA 2 (Adar2), an A-to-I RNA editing enzyme catalyzing rhythmic A-to-I RNA editing, governs a wide range of mRNA rhythms in the mouse liver and regulates the circadian behavior. In brain, ADAR2-mediated A-to-I RNA editing was reported to occur in various transcripts encoding ion channels and neurotransmitter receptors, which could influence neuronal function of the SCN. Here we show that ADAR2 plays a crucial role for light-induced phase-shift of the circadian clock. Intriguingly, exposure of Adar2-knockout mice to a light pulse at late night caused an aberrant phase-advance of the locomotor rhythms. By monitoring the bioluminescence rhythms of the mutant SCN slices, we found that a phase-advance induced by treatment with pituitary adenylyl cyclase-activating polypeptide (PACAP) was markedly attenuated. The present study suggests that A-to-I RNA editing in the SCN regulates a proper phase response to light in the mouse circadian system.Hideki TerajimaHikari YoshitaneTomoko YoshikawaYasufumi ShigeyoshiYoshitaka FukadaNature PortfolioarticlePituitary Adenylyl Cyclase-activating Polypeptide (PACAP)ADAR2 Knockout MiceBioluminescence RhythmLight-induced Phase AdvancesmRNA RhythmMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pituitary Adenylyl Cyclase-activating Polypeptide (PACAP) ADAR2 Knockout Mice Bioluminescence Rhythm Light-induced Phase Advances mRNA Rhythm Medicine R Science Q
spellingShingle	Pituitary Adenylyl Cyclase-activating Polypeptide (PACAP) ADAR2 Knockout Mice Bioluminescence Rhythm Light-induced Phase Advances mRNA Rhythm Medicine R Science Q Hideki Terajima Hikari Yoshitane Tomoko Yoshikawa Yasufumi Shigeyoshi Yoshitaka Fukada A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
description	Abstract In mammals, the central circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and it orchestrates peripheral clocks in the whole body to organize physiological and behavioral rhythms. Light-induced phase-shift of the SCN clock enables synchronization of the circadian clock system with 24-h environmental light/dark cycle. We previously found that adenosine deaminase acting on RNA 2 (Adar2), an A-to-I RNA editing enzyme catalyzing rhythmic A-to-I RNA editing, governs a wide range of mRNA rhythms in the mouse liver and regulates the circadian behavior. In brain, ADAR2-mediated A-to-I RNA editing was reported to occur in various transcripts encoding ion channels and neurotransmitter receptors, which could influence neuronal function of the SCN. Here we show that ADAR2 plays a crucial role for light-induced phase-shift of the circadian clock. Intriguingly, exposure of Adar2-knockout mice to a light pulse at late night caused an aberrant phase-advance of the locomotor rhythms. By monitoring the bioluminescence rhythms of the mutant SCN slices, we found that a phase-advance induced by treatment with pituitary adenylyl cyclase-activating polypeptide (PACAP) was markedly attenuated. The present study suggests that A-to-I RNA editing in the SCN regulates a proper phase response to light in the mouse circadian system.
format	article
author	Hideki Terajima Hikari Yoshitane Tomoko Yoshikawa Yasufumi Shigeyoshi Yoshitaka Fukada
author_facet	Hideki Terajima Hikari Yoshitane Tomoko Yoshikawa Yasufumi Shigeyoshi Yoshitaka Fukada
author_sort	Hideki Terajima
title	A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
title_short	A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
title_full	A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
title_fullStr	A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
title_full_unstemmed	A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift
title_sort	a-to-i rna editing enzyme adar2 regulates light-induced circadian phase-shift
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2306fa7987464a5eb119a4d7b722dfab
work_keys_str_mv	AT hidekiterajima atoirnaeditingenzymeadar2regulateslightinducedcircadianphaseshift AT hikariyoshitane atoirnaeditingenzymeadar2regulateslightinducedcircadianphaseshift AT tomokoyoshikawa atoirnaeditingenzymeadar2regulateslightinducedcircadianphaseshift AT yasufumishigeyoshi atoirnaeditingenzymeadar2regulateslightinducedcircadianphaseshift AT yoshitakafukada atoirnaeditingenzymeadar2regulateslightinducedcircadianphaseshift
_version_	1718395388197601280

A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift

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