Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106

Abstract Cellulase (mainly endoglucanase, EG) has been used in pulp modification for improving paper quality through environmentally friendly process. But low activity in alkaline pH and high filter paper activity (FPA) were still obstacles for extending the cellulase application in papermaking indu...

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Autores principales:	Meimei Wang, Jian Du, Daolei Zhang, Xuezhi Li, Jian Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23077705c8004ba0a4be44fa77b4691c
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spelling	oai:doaj.org-article:23077705c8004ba0a4be44fa77b4691c2021-12-02T15:06:07ZModification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y10610.1038/s41598-017-03215-92045-2322https://doaj.org/article/23077705c8004ba0a4be44fa77b4691c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03215-9https://doaj.org/toc/2045-2322Abstract Cellulase (mainly endoglucanase, EG) has been used in pulp modification for improving paper quality through environmentally friendly process. But low activity in alkaline pH and high filter paper activity (FPA) were still obstacles for extending the cellulase application in papermaking industry. In the study, an alkali-tolerant EG gene of Bacillus subtilis Y106 was homologous over-expressed for obtaining suitable enzyme used in pulp modification. The engineering strain could produce the crude enzyme with more alkali-tolerant EG and little PFA. Potential of the crude enzyme in modification of different pulps were investigated. It was found that the enzyme could be used for improving drainage and strength properties of pulps from softwood, hardwood and non-wood materials, especially non-wood pulp such as wheat straw pulp. The underlying mechanisms of pulp modification and different effects on various types of pulps by the EG treatment were also discussed by studying the change in fibers characteristics and fiber bonding.Meimei WangJian DuDaolei ZhangXuezhi LiJian ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meimei Wang Jian Du Daolei Zhang Xuezhi Li Jian Zhao Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
description	Abstract Cellulase (mainly endoglucanase, EG) has been used in pulp modification for improving paper quality through environmentally friendly process. But low activity in alkaline pH and high filter paper activity (FPA) were still obstacles for extending the cellulase application in papermaking industry. In the study, an alkali-tolerant EG gene of Bacillus subtilis Y106 was homologous over-expressed for obtaining suitable enzyme used in pulp modification. The engineering strain could produce the crude enzyme with more alkali-tolerant EG and little PFA. Potential of the crude enzyme in modification of different pulps were investigated. It was found that the enzyme could be used for improving drainage and strength properties of pulps from softwood, hardwood and non-wood materials, especially non-wood pulp such as wheat straw pulp. The underlying mechanisms of pulp modification and different effects on various types of pulps by the EG treatment were also discussed by studying the change in fibers characteristics and fiber bonding.
format	article
author	Meimei Wang Jian Du Daolei Zhang Xuezhi Li Jian Zhao
author_facet	Meimei Wang Jian Du Daolei Zhang Xuezhi Li Jian Zhao
author_sort	Meimei Wang
title	Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
title_short	Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
title_full	Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
title_fullStr	Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
title_full_unstemmed	Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106
title_sort	modification of different pulps by homologous overexpression alkali-tolerant endoglucanase in bacillus subtilis y106
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/23077705c8004ba0a4be44fa77b4691c
work_keys_str_mv	AT meimeiwang modificationofdifferentpulpsbyhomologousoverexpressionalkalitolerantendoglucanaseinbacillussubtilisy106 AT jiandu modificationofdifferentpulpsbyhomologousoverexpressionalkalitolerantendoglucanaseinbacillussubtilisy106 AT daoleizhang modificationofdifferentpulpsbyhomologousoverexpressionalkalitolerantendoglucanaseinbacillussubtilisy106 AT xuezhili modificationofdifferentpulpsbyhomologousoverexpressionalkalitolerantendoglucanaseinbacillussubtilisy106 AT jianzhao modificationofdifferentpulpsbyhomologousoverexpressionalkalitolerantendoglucanaseinbacillussubtilisy106
_version_	1718388565265612800

Modification of Different Pulps by Homologous Overexpression Alkali-Tolerant Endoglucanase in Bacillus subtilis Y106

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