Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.

Nucleotide excision repair (NER) orchestrates the repair of helix distorting DNA damage, induced by both ultraviolet radiation (UVR) and cisplatin. There is evidence that the global genome repair (GGR) arm of NER is dysfunctional in melanoma and it is known to have limited induction in melanoma cell...

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Autores principales:	Nikola A Bowden, Katie A Ashton, Ricardo E Vilain, Kelly A Avery-Kiejda, Ryan J Davey, Heather C Murray, Timothy Budden, Stephen G Braye, Xu Dong Zhang, Peter Hersey, Rodney J Scott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/230d2908070146acaf291933e25de21c
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spelling	oai:doaj.org-article:230d2908070146acaf291933e25de21c2021-11-18T09:01:22ZRegulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.1932-620310.1371/journal.pone.0070424https://doaj.org/article/230d2908070146acaf291933e25de21c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940574/?tool=EBIhttps://doaj.org/toc/1932-6203Nucleotide excision repair (NER) orchestrates the repair of helix distorting DNA damage, induced by both ultraviolet radiation (UVR) and cisplatin. There is evidence that the global genome repair (GGR) arm of NER is dysfunctional in melanoma and it is known to have limited induction in melanoma cell lines after cisplatin treatment. The aims of this study were to examine mRNA transcript levels of regulators of GGR and to investigate the downstream effect on global transcript expression in melanoma cell lines after cisplatin treatment and in melanoma tumours. The GGR regulators, BRCA1 and PCNA, were induced in melanocytes after cisplatin, but not in melanoma cell lines. Transcripts associated with BRCA1, BRCA2, ATM and CHEK2 showed altered expression in melanoma cell lines after cisplatin treatment. In melanoma tumour tissue BRCA1 transcript expression correlated with poor survival and XPB expression correlated with solar elastosis levels. Taken together, these findings provide evidence of the mechanisms underlying NER deficiency in melanoma.Nikola A BowdenKatie A AshtonRicardo E VilainKelly A Avery-KiejdaRyan J DaveyHeather C MurrayTimothy BuddenStephen G BrayeXu Dong ZhangPeter HerseyRodney J ScottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70424 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nikola A Bowden Katie A Ashton Ricardo E Vilain Kelly A Avery-Kiejda Ryan J Davey Heather C Murray Timothy Budden Stephen G Braye Xu Dong Zhang Peter Hersey Rodney J Scott Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
description	Nucleotide excision repair (NER) orchestrates the repair of helix distorting DNA damage, induced by both ultraviolet radiation (UVR) and cisplatin. There is evidence that the global genome repair (GGR) arm of NER is dysfunctional in melanoma and it is known to have limited induction in melanoma cell lines after cisplatin treatment. The aims of this study were to examine mRNA transcript levels of regulators of GGR and to investigate the downstream effect on global transcript expression in melanoma cell lines after cisplatin treatment and in melanoma tumours. The GGR regulators, BRCA1 and PCNA, were induced in melanocytes after cisplatin, but not in melanoma cell lines. Transcripts associated with BRCA1, BRCA2, ATM and CHEK2 showed altered expression in melanoma cell lines after cisplatin treatment. In melanoma tumour tissue BRCA1 transcript expression correlated with poor survival and XPB expression correlated with solar elastosis levels. Taken together, these findings provide evidence of the mechanisms underlying NER deficiency in melanoma.
format	article
author	Nikola A Bowden Katie A Ashton Ricardo E Vilain Kelly A Avery-Kiejda Ryan J Davey Heather C Murray Timothy Budden Stephen G Braye Xu Dong Zhang Peter Hersey Rodney J Scott
author_facet	Nikola A Bowden Katie A Ashton Ricardo E Vilain Kelly A Avery-Kiejda Ryan J Davey Heather C Murray Timothy Budden Stephen G Braye Xu Dong Zhang Peter Hersey Rodney J Scott
author_sort	Nikola A Bowden
title	Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
title_short	Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
title_full	Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
title_fullStr	Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
title_full_unstemmed	Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.
title_sort	regulators of global genome repair do not respond to dna damaging therapy but correlate with survival in melanoma.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/230d2908070146acaf291933e25de21c
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Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.

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