Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

Abstract Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual...

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Autores principales:	Kanako Saito, Yuri Ota, Dieter M. Tourlousse, Satoko Matsukura, Hirotsugu Fujitani, Masamune Morita, Satoshi Tsuneda, Naohiro Noda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/230e74c3fab4425fa287854808e13434
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spelling	oai:doaj.org-article:230e74c3fab4425fa287854808e134342021-12-02T15:37:58ZMicrodroplet-based system for culturing of environmental microorganisms using FNAP-sort10.1038/s41598-021-88974-22045-2322https://doaj.org/article/230e74c3fab4425fa287854808e134342021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88974-2https://doaj.org/toc/2045-2322Abstract Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.Kanako SaitoYuri OtaDieter M. TourlousseSatoko MatsukuraHirotsugu FujitaniMasamune MoritaSatoshi TsunedaNaohiro NodaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kanako Saito Yuri Ota Dieter M. Tourlousse Satoko Matsukura Hirotsugu Fujitani Masamune Morita Satoshi Tsuneda Naohiro Noda Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
description	Abstract Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.
format	article
author	Kanako Saito Yuri Ota Dieter M. Tourlousse Satoko Matsukura Hirotsugu Fujitani Masamune Morita Satoshi Tsuneda Naohiro Noda
author_facet	Kanako Saito Yuri Ota Dieter M. Tourlousse Satoko Matsukura Hirotsugu Fujitani Masamune Morita Satoshi Tsuneda Naohiro Noda
author_sort	Kanako Saito
title	Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
title_short	Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
title_full	Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
title_fullStr	Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
title_full_unstemmed	Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
title_sort	microdroplet-based system for culturing of environmental microorganisms using fnap-sort
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/230e74c3fab4425fa287854808e13434
work_keys_str_mv	AT kanakosaito microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT yuriota microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT dietermtourlousse microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT satokomatsukura microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT hirotsugufujitani microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT masamunemorita microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT satoshitsuneda microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort AT naohironoda microdropletbasedsystemforculturingofenvironmentalmicroorganismsusingfnapsort
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Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

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