De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits
Brown adipose tissues (BATs) convert to a “white-like” phenotype with age, which is also known as “aging-related BAT whitening (ARBW)”. Emerging evidence suggested that long non-coding RNAs (lncRNAs) were widely involved in adipose biology. Rabbit is an ideal model for studying the dynamics of ARBW....
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2021
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oai:doaj.org-article:23145356dd8a4157bfcf9f26666149132021-11-25T16:47:44ZDe Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits10.3390/biology101111762079-7737https://doaj.org/article/23145356dd8a4157bfcf9f26666149132021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1176https://doaj.org/toc/2079-7737Brown adipose tissues (BATs) convert to a “white-like” phenotype with age, which is also known as “aging-related BAT whitening (ARBW)”. Emerging evidence suggested that long non-coding RNAs (lncRNAs) were widely involved in adipose biology. Rabbit is an ideal model for studying the dynamics of ARBW. In this study, we performed histological analysis and strand-specific RNA-sequencing (ssRNA-seq) of rabbit interscapular adipose tissues (iATs). Our data indicated that the rabbit iATs underwent the ARBW from 0 days to 2 years and a total of 2281 novel lncRNAs were identified in the iATs. The classical rabbit BATs showed low lncRNA transcriptional complexity compared to white adipose tissues (WATs). A total of 631 differentially expressed lncRNAs (DELs) were identified in four stages. The signal pathways of purine metabolism, Wnt signaling pathway, peroxisome proliferator-activated receptor (PPAR) signaling pathway, cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (cGMP-PKG) signaling pathway and lipid and atherosclerosis were significantly enriched by the DELs with unique expression patterns. A novel lncRNA that was highly expressed in the iATs of aged rabbits was validated to impair brown adipocyte differentiation in vitro. Our study provided a comprehensive catalog of lncRNAs involved in ARBW in rabbits, which facilitates a better understanding of adipose biology.Kun DuXue BaiLi YangYu ShiLi ChenHaoding WangMingchen CaiJie WangShiyi ChenXianbo JiaSongjia LaiMDPI AGarticleBATsrabbitsssRNA-seqhomeothermic abilitywhiteningBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1176, p 1176 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
BATs rabbits ssRNA-seq homeothermic ability whitening Biology (General) QH301-705.5 |
| spellingShingle |
BATs rabbits ssRNA-seq homeothermic ability whitening Biology (General) QH301-705.5 Kun Du Xue Bai Li Yang Yu Shi Li Chen Haoding Wang Mingchen Cai Jie Wang Shiyi Chen Xianbo Jia Songjia Lai De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| description |
Brown adipose tissues (BATs) convert to a “white-like” phenotype with age, which is also known as “aging-related BAT whitening (ARBW)”. Emerging evidence suggested that long non-coding RNAs (lncRNAs) were widely involved in adipose biology. Rabbit is an ideal model for studying the dynamics of ARBW. In this study, we performed histological analysis and strand-specific RNA-sequencing (ssRNA-seq) of rabbit interscapular adipose tissues (iATs). Our data indicated that the rabbit iATs underwent the ARBW from 0 days to 2 years and a total of 2281 novel lncRNAs were identified in the iATs. The classical rabbit BATs showed low lncRNA transcriptional complexity compared to white adipose tissues (WATs). A total of 631 differentially expressed lncRNAs (DELs) were identified in four stages. The signal pathways of purine metabolism, Wnt signaling pathway, peroxisome proliferator-activated receptor (PPAR) signaling pathway, cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (cGMP-PKG) signaling pathway and lipid and atherosclerosis were significantly enriched by the DELs with unique expression patterns. A novel lncRNA that was highly expressed in the iATs of aged rabbits was validated to impair brown adipocyte differentiation in vitro. Our study provided a comprehensive catalog of lncRNAs involved in ARBW in rabbits, which facilitates a better understanding of adipose biology. |
| format |
article |
| author |
Kun Du Xue Bai Li Yang Yu Shi Li Chen Haoding Wang Mingchen Cai Jie Wang Shiyi Chen Xianbo Jia Songjia Lai |
| author_facet |
Kun Du Xue Bai Li Yang Yu Shi Li Chen Haoding Wang Mingchen Cai Jie Wang Shiyi Chen Xianbo Jia Songjia Lai |
| author_sort |
Kun Du |
| title |
De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| title_short |
De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| title_full |
De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| title_fullStr |
De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| title_full_unstemmed |
De Novo Reconstruction of Transcriptome Identified Long Non-Coding RNA Regulator of Aging-Related Brown Adipose Tissue Whitening in Rabbits |
| title_sort |
de novo reconstruction of transcriptome identified long non-coding rna regulator of aging-related brown adipose tissue whitening in rabbits |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/23145356dd8a4157bfcf9f2666614913 |
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