The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots

Lu-Lu Qiao, Wen-Jing Yao, Zhi-Qiang Zhang, Xiaojing Yang, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering of Henan Province, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineer...

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Autores principales:	Qiao LL, Yao WJ, Zhang ZQ, Yang X, Zhao MX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	quantum dots 5-fluorouracil nano drugs apoptosis antitumor activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/23361e01e63a46faa282452e7d171a82
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spelling	oai:doaj.org-article:23361e01e63a46faa282452e7d171a822021-12-02T08:54:58ZThe Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots1178-2013https://doaj.org/article/23361e01e63a46faa282452e7d171a822020-04-01T00:00:00Zhttps://www.dovepress.com/the-biological-activity-research-of-the-nano-drugs-based-on-5-fluorour-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lu-Lu Qiao, Wen-Jing Yao, Zhi-Qiang Zhang, Xiaojing Yang, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering of Henan Province, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering of Henan Province, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Over the past decades, quantum dots (QDs) have shown the broad application in diverse fields, especially in intracellular probing and drug delivery, due to their high fluorescence intensity, long fluorescence lifetime, strong light-resistant bleaching ability, and strong light stability. Therefore, we explore a kind of therapeutic potential against cancer with fluorescent imaging.Methods: In the current study, a new type of QDs (QDs@L-Cys-TAEA-5-FUA) capped with L-cysteine (L-Cys) and tris(2-aminoethyl)amine (TAEA) ligands, and conjugated with 5-fluorouracil-1-acetic acid (5-FUA) has been synthesized. Ligands were characterized by electrospray ionization mass spectrometry and H-nuclear magnetic resonance (1H NMR) spectroscopy. The modified QDs were characterized by transmission electron microscopy, ultraviolet and visible spectrophotometry (UV-Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 HepG2, and QSG-7701 cells. The fluorescence imaging of modified QDs was obtained by fluorescence microscope.Results: The modified QDs are of controllable sizes in the range of 4– 5 nm and they possess strong optical emission properties. UV-Vis and fluorescence spectra demonstrated that the L-Cys-TAEA-5-FUA was successfully incorporated into QD nanoparticles. The MTT results demonstrated that L-Cys-TAEA-5-FUA modified QDs could efficiently inhibit the proliferation of cancer cells as compared to the normal cells, illustrating their antitumor efficacy. The mechanistic studies revealed that the effective internalization of modified QDs inside cancer cells could inhibit their proliferation, through excessive production of intracellular reactive oxygen species, leading to apoptosis process.Conclusion: The present study suggests that modified QDs can enter cells efficiently and could be employed as therapeutic agents for the treatment of various types of cancers with fluorescent imaging.Keywords: quantum dots, 5-fluorouracil, nano-drugs, apoptosis, antitumor activityQiao LLYao WJZhang ZQYang XZhao MXDove Medical Pressarticlequantum dots5-fluorouracilnano drugsapoptosisantitumor activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2765-2776 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantum dots 5-fluorouracil nano drugs apoptosis antitumor activity Medicine (General) R5-920
spellingShingle	quantum dots 5-fluorouracil nano drugs apoptosis antitumor activity Medicine (General) R5-920 Qiao LL Yao WJ Zhang ZQ Yang X Zhao MX The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
description	Lu-Lu Qiao, Wen-Jing Yao, Zhi-Qiang Zhang, Xiaojing Yang, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering of Henan Province, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering of Henan Province, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Over the past decades, quantum dots (QDs) have shown the broad application in diverse fields, especially in intracellular probing and drug delivery, due to their high fluorescence intensity, long fluorescence lifetime, strong light-resistant bleaching ability, and strong light stability. Therefore, we explore a kind of therapeutic potential against cancer with fluorescent imaging.Methods: In the current study, a new type of QDs (QDs@L-Cys-TAEA-5-FUA) capped with L-cysteine (L-Cys) and tris(2-aminoethyl)amine (TAEA) ligands, and conjugated with 5-fluorouracil-1-acetic acid (5-FUA) has been synthesized. Ligands were characterized by electrospray ionization mass spectrometry and H-nuclear magnetic resonance (1H NMR) spectroscopy. The modified QDs were characterized by transmission electron microscopy, ultraviolet and visible spectrophotometry (UV-Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 HepG2, and QSG-7701 cells. The fluorescence imaging of modified QDs was obtained by fluorescence microscope.Results: The modified QDs are of controllable sizes in the range of 4– 5 nm and they possess strong optical emission properties. UV-Vis and fluorescence spectra demonstrated that the L-Cys-TAEA-5-FUA was successfully incorporated into QD nanoparticles. The MTT results demonstrated that L-Cys-TAEA-5-FUA modified QDs could efficiently inhibit the proliferation of cancer cells as compared to the normal cells, illustrating their antitumor efficacy. The mechanistic studies revealed that the effective internalization of modified QDs inside cancer cells could inhibit their proliferation, through excessive production of intracellular reactive oxygen species, leading to apoptosis process.Conclusion: The present study suggests that modified QDs can enter cells efficiently and could be employed as therapeutic agents for the treatment of various types of cancers with fluorescent imaging.Keywords: quantum dots, 5-fluorouracil, nano-drugs, apoptosis, antitumor activity
format	article
author	Qiao LL Yao WJ Zhang ZQ Yang X Zhao MX
author_facet	Qiao LL Yao WJ Zhang ZQ Yang X Zhao MX
author_sort	Qiao LL
title	The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
title_short	The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
title_full	The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
title_fullStr	The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
title_full_unstemmed	The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots
title_sort	biological activity research of the nano-drugs based on 5-fluorouracil-modified quantum dots
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/23361e01e63a46faa282452e7d171a82
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The Biological Activity Research of the Nano-Drugs Based on 5-Fluorouracil-Modified Quantum Dots

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