Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.

Triglycerides have a limited solubility, around 3%, in phosphatidylcholine lipid bilayers. Using millisecond-scale course grained molecular dynamics simulations, we show that the model lipid bilayer can accommodate a higher concentration of triolein (TO) than earlier anticipated, by sequestering tri...

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Autores principales:	Himanshu Khandelia, Lars Duelund, Kirsi I Pakkanen, John H Ipsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/233808a5b8314a4e9135f28429c02918
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spelling	oai:doaj.org-article:233808a5b8314a4e9135f28429c029182021-11-18T06:34:55ZTriglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.1932-620310.1371/journal.pone.0012811https://doaj.org/article/233808a5b8314a4e9135f28429c029182010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20877640/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Triglycerides have a limited solubility, around 3%, in phosphatidylcholine lipid bilayers. Using millisecond-scale course grained molecular dynamics simulations, we show that the model lipid bilayer can accommodate a higher concentration of triolein (TO) than earlier anticipated, by sequestering triolein molecules to the bilayer center in the form of a disordered, isotropic, mobile neutral lipid aggregate, at least 17 nm in diameter, which forms spontaneously, and remains stable on at least the microsecond time scale. The results give credence to the hotly debated existence of mobile neutral lipid aggregates of unknown function present in malignant cells, and to the early biogenesis of lipid droplets accommodated between the two leaflets of the endoplasmic reticulum membrane. The TO aggregates give the bilayer a blister-like appearance, and will hinder the formation of multi-lamellar phases in model, and possibly living membranes. The blisters will result in anomalous membrane probe partitioning, which should be accounted for in the interpretation of probe-related measurements.Himanshu KhandeliaLars DuelundKirsi I PakkanenJohn H IpsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12811 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Himanshu Khandelia Lars Duelund Kirsi I Pakkanen John H Ipsen Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
description	Triglycerides have a limited solubility, around 3%, in phosphatidylcholine lipid bilayers. Using millisecond-scale course grained molecular dynamics simulations, we show that the model lipid bilayer can accommodate a higher concentration of triolein (TO) than earlier anticipated, by sequestering triolein molecules to the bilayer center in the form of a disordered, isotropic, mobile neutral lipid aggregate, at least 17 nm in diameter, which forms spontaneously, and remains stable on at least the microsecond time scale. The results give credence to the hotly debated existence of mobile neutral lipid aggregates of unknown function present in malignant cells, and to the early biogenesis of lipid droplets accommodated between the two leaflets of the endoplasmic reticulum membrane. The TO aggregates give the bilayer a blister-like appearance, and will hinder the formation of multi-lamellar phases in model, and possibly living membranes. The blisters will result in anomalous membrane probe partitioning, which should be accounted for in the interpretation of probe-related measurements.
format	article
author	Himanshu Khandelia Lars Duelund Kirsi I Pakkanen John H Ipsen
author_facet	Himanshu Khandelia Lars Duelund Kirsi I Pakkanen John H Ipsen
author_sort	Himanshu Khandelia
title	Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
title_short	Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
title_full	Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
title_fullStr	Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
title_full_unstemmed	Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
title_sort	triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/233808a5b8314a4e9135f28429c02918
work_keys_str_mv	AT himanshukhandelia triglycerideblistersinlipidbilayersimplicationsforlipiddropletbiogenesisandthemobilelipidsignalincancercellmembranes AT larsduelund triglycerideblistersinlipidbilayersimplicationsforlipiddropletbiogenesisandthemobilelipidsignalincancercellmembranes AT kirsiipakkanen triglycerideblistersinlipidbilayersimplicationsforlipiddropletbiogenesisandthemobilelipidsignalincancercellmembranes AT johnhipsen triglycerideblistersinlipidbilayersimplicationsforlipiddropletbiogenesisandthemobilelipidsignalincancercellmembranes
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Triglyceride blisters in lipid bilayers: implications for lipid droplet biogenesis and the mobile lipid signal in cancer cell membranes.

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