Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis. The combination of hardware modification and data analysis allows classification of cell cycle subsets without needing h...

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Autores principales: Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/233a6ba2a990438f8bf02511361ce7c9
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spelling oai:doaj.org-article:233a6ba2a990438f8bf02511361ce7c92021-12-02T19:16:53ZMulti-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification10.1038/s42003-021-02664-32399-3642https://doaj.org/article/233a6ba2a990438f8bf02511361ce7c92021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02664-3https://doaj.org/toc/2399-3642Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis. The combination of hardware modification and data analysis allows classification of cell cycle subsets without needing harmful cell labelling.Daniel KageKerstin HeinrichKonrad v. VolkmannJenny KirschKristen FeherClaudia Giesecke-ThielToralf KaiserNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Daniel Kage
Kerstin Heinrich
Konrad v. Volkmann
Jenny Kirsch
Kristen Feher
Claudia Giesecke-Thiel
Toralf Kaiser
Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
description Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis. The combination of hardware modification and data analysis allows classification of cell cycle subsets without needing harmful cell labelling.
format article
author Daniel Kage
Kerstin Heinrich
Konrad v. Volkmann
Jenny Kirsch
Kristen Feher
Claudia Giesecke-Thiel
Toralf Kaiser
author_facet Daniel Kage
Kerstin Heinrich
Konrad v. Volkmann
Jenny Kirsch
Kristen Feher
Claudia Giesecke-Thiel
Toralf Kaiser
author_sort Daniel Kage
title Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
title_short Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
title_full Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
title_fullStr Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
title_full_unstemmed Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
title_sort multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/233a6ba2a990438f8bf02511361ce7c9
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