Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing

Abstract Advances in whole genome amplification (WGA) techniques enable understanding of the genomic sequence at a single cell level. Demand for single cell dedicated WGA kits (scWGA) has led to the development of several commercial kit. To this point, no robust comparison of all available kits was...

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Autores principales:	Tamir Biezuner, Ofir Raz, Shiran Amir, Lilach Milo, Rivka Adar, Yael Fried, Elena Ainbinder, Ehud Shapiro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/233cc7220ab840d999bad1b29759c3fe
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spelling	oai:doaj.org-article:233cc7220ab840d999bad1b29759c3fe2021-12-02T18:53:14ZComparison of seven single cell whole genome amplification commercial kits using targeted sequencing10.1038/s41598-021-96045-92045-2322https://doaj.org/article/233cc7220ab840d999bad1b29759c3fe2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96045-9https://doaj.org/toc/2045-2322Abstract Advances in whole genome amplification (WGA) techniques enable understanding of the genomic sequence at a single cell level. Demand for single cell dedicated WGA kits (scWGA) has led to the development of several commercial kit. To this point, no robust comparison of all available kits was performed. Here, we benchmark an economical assay, comparing all commercially available scWGA kits. Our comparison is based on targeted sequencing of thousands of genomic loci, including highly mutable regions, from a large cohort of human single cells. Using this approach we have demonstrated the superiority of Ampli1 in genome coverage and of RepliG in reduced error rate. In summary, we show that no single kit is optimal across all categories, highlighting the need for a dedicated kit selection in accordance with experimental requirements.Tamir BiezunerOfir RazShiran AmirLilach MiloRivka AdarYael FriedElena AinbinderEhud ShapiroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tamir Biezuner Ofir Raz Shiran Amir Lilach Milo Rivka Adar Yael Fried Elena Ainbinder Ehud Shapiro Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
description	Abstract Advances in whole genome amplification (WGA) techniques enable understanding of the genomic sequence at a single cell level. Demand for single cell dedicated WGA kits (scWGA) has led to the development of several commercial kit. To this point, no robust comparison of all available kits was performed. Here, we benchmark an economical assay, comparing all commercially available scWGA kits. Our comparison is based on targeted sequencing of thousands of genomic loci, including highly mutable regions, from a large cohort of human single cells. Using this approach we have demonstrated the superiority of Ampli1 in genome coverage and of RepliG in reduced error rate. In summary, we show that no single kit is optimal across all categories, highlighting the need for a dedicated kit selection in accordance with experimental requirements.
format	article
author	Tamir Biezuner Ofir Raz Shiran Amir Lilach Milo Rivka Adar Yael Fried Elena Ainbinder Ehud Shapiro
author_facet	Tamir Biezuner Ofir Raz Shiran Amir Lilach Milo Rivka Adar Yael Fried Elena Ainbinder Ehud Shapiro
author_sort	Tamir Biezuner
title	Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
title_short	Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
title_full	Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
title_fullStr	Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
title_full_unstemmed	Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
title_sort	comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/233cc7220ab840d999bad1b29759c3fe
work_keys_str_mv	AT tamirbiezuner comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT ofirraz comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT shiranamir comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT lilachmilo comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT rivkaadar comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT yaelfried comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT elenaainbinder comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing AT ehudshapiro comparisonofsevensinglecellwholegenomeamplificationcommercialkitsusingtargetedsequencing
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Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing

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