Screening and Molecular Identification of Bacteria from the Midgut of Amphimallon solstitiale Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes

Entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) are a group of organisms capable of infecting larvae of insects living in soil, including representatives of the family Scarabaeidae. Their insecticidal activity i...

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Autores principales:	Marcin Skowronek, Ewa Sajnaga, Waldemar Kazimierczak, Magdalena Lis, Adrian Wiater
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	midgut microbiota bacterial interactions entomopathogenic nematodes <i>Xenorhabdus</i> <i>Photorhabdus</i> <i>Amphimallon solstitiale</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/233fbc39b7d7402db1b39ba3d03d5a41
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spelling	oai:doaj.org-article:233fbc39b7d7402db1b39ba3d03d5a412021-11-11T17:25:30ZScreening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes10.3390/ijms2221120051422-00671661-6596https://doaj.org/article/233fbc39b7d7402db1b39ba3d03d5a412021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12005https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Entomopathogenic nematodes (Rhabditida: <i>Steinernematidae</i> and <i>Heterorhabditidae</i>) are a group of organisms capable of infecting larvae of insects living in soil, including representatives of the family <i>Scarabaeidae</i>. Their insecticidal activity is related to the presence of symbiotic bacteria <i>Xenorhabdus</i> spp. or <i>Photorhabdus</i> spp. in the alimentary tract, which are released into the insect body, leading to its death caused by bacterial toxins and septicemia. Although the antibacterial activities of symbionts of entomopathogenic nematodes have been well described, there is insufficient knowledge of the interactions between these bacteria and microorganisms that naturally inhabit the alimentary tract of insects infested by nematodes. In this study, 900 bacterial strains isolated from midgut samples of <i>Amphimallon solstitiale</i> larvae were tested for their antagonistic activity against the selected five <i>Xenorhabdus</i> and <i>Photorhabdus</i> species. Cross-streak tests showed significant antibacterial activity of 20 isolates. These bacteria were identified as <i>Bacillus [Brevibacterium] frigoritolerans</i>, <i>Bacillus toyonensis</i>, <i>Bacillus wiedmannii</i>, <i>Chryseobacterium lathyri</i>, <i>Chryseobacterium</i> sp., <i>Citrobacter murliniae</i>, <i>Enterococcus malodoratus</i>, <i>Paenibacillus</i> sp., <i>Serratia marcescens</i> and <i>Serratia</i> sp. Since some representatives of the intestinal microbiota of <i>A. solstitiale</i> are able to inhibit the growth of <i>Xenorhabdus</i> and <i>Photorhrhabdus</i> bacteria in vitro, it can be assumed that this type of bacterial interaction may occur at certain stages of insect infection by <i>Steinernema</i> or <i>Heterorhabditis</i> nematodes.Marcin SkowronekEwa SajnagaWaldemar KazimierczakMagdalena LisAdrian WiaterMDPI AGarticlemidgut microbiotabacterial interactionsentomopathogenic nematodes<i>Xenorhabdus</i><i>Photorhabdus</i><i>Amphimallon solstitiale</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12005, p 12005 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	midgut microbiota bacterial interactions entomopathogenic nematodes <i>Xenorhabdus</i> <i>Photorhabdus</i> <i>Amphimallon solstitiale</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	midgut microbiota bacterial interactions entomopathogenic nematodes <i>Xenorhabdus</i> <i>Photorhabdus</i> <i>Amphimallon solstitiale</i> Biology (General) QH301-705.5 Chemistry QD1-999 Marcin Skowronek Ewa Sajnaga Waldemar Kazimierczak Magdalena Lis Adrian Wiater Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
description	Entomopathogenic nematodes (Rhabditida: <i>Steinernematidae</i> and <i>Heterorhabditidae</i>) are a group of organisms capable of infecting larvae of insects living in soil, including representatives of the family <i>Scarabaeidae</i>. Their insecticidal activity is related to the presence of symbiotic bacteria <i>Xenorhabdus</i> spp. or <i>Photorhabdus</i> spp. in the alimentary tract, which are released into the insect body, leading to its death caused by bacterial toxins and septicemia. Although the antibacterial activities of symbionts of entomopathogenic nematodes have been well described, there is insufficient knowledge of the interactions between these bacteria and microorganisms that naturally inhabit the alimentary tract of insects infested by nematodes. In this study, 900 bacterial strains isolated from midgut samples of <i>Amphimallon solstitiale</i> larvae were tested for their antagonistic activity against the selected five <i>Xenorhabdus</i> and <i>Photorhabdus</i> species. Cross-streak tests showed significant antibacterial activity of 20 isolates. These bacteria were identified as <i>Bacillus [Brevibacterium] frigoritolerans</i>, <i>Bacillus toyonensis</i>, <i>Bacillus wiedmannii</i>, <i>Chryseobacterium lathyri</i>, <i>Chryseobacterium</i> sp., <i>Citrobacter murliniae</i>, <i>Enterococcus malodoratus</i>, <i>Paenibacillus</i> sp., <i>Serratia marcescens</i> and <i>Serratia</i> sp. Since some representatives of the intestinal microbiota of <i>A. solstitiale</i> are able to inhibit the growth of <i>Xenorhabdus</i> and <i>Photorhrhabdus</i> bacteria in vitro, it can be assumed that this type of bacterial interaction may occur at certain stages of insect infection by <i>Steinernema</i> or <i>Heterorhabditis</i> nematodes.
format	article
author	Marcin Skowronek Ewa Sajnaga Waldemar Kazimierczak Magdalena Lis Adrian Wiater
author_facet	Marcin Skowronek Ewa Sajnaga Waldemar Kazimierczak Magdalena Lis Adrian Wiater
author_sort	Marcin Skowronek
title	Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
title_short	Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
title_full	Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
title_fullStr	Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
title_full_unstemmed	Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes
title_sort	screening and molecular identification of bacteria from the midgut of <i>amphimallon solstitiale</i> larvae exhibiting antagonistic activity against bacterial symbionts of entomopathogenic nematodes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/233fbc39b7d7402db1b39ba3d03d5a41
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Screening and Molecular Identification of Bacteria from the Midgut of <i>Amphimallon solstitiale</i> Larvae Exhibiting Antagonistic Activity against Bacterial Symbionts of Entomopathogenic Nematodes

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