Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge

Abstract Recent studies have shown that transforming microglia phenotype from pro-inflammation of M1 phenotype to anti-inflammation and tissue-repairing M2 phenotype may be an effective therapeutic strategy for preventing ischemic stroke brain injury. Isosteviol Sodium (STV-Na) has shown promise as...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hao Zhang, Minyi Lu, Xiaofeng Zhang, Yihe Kuai, Ying Mei, Qiwen Tan, Kailun Zhong, Xiaoou Sun, Wen Tan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/234276ff02a14fe88e368e0c70c27f0a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:234276ff02a14fe88e368e0c70c27f0a
record_format	dspace
spelling	oai:doaj.org-article:234276ff02a14fe88e368e0c70c27f0a2021-12-02T15:08:47ZIsosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge10.1038/s41598-019-48759-02045-2322https://doaj.org/article/234276ff02a14fe88e368e0c70c27f0a2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48759-0https://doaj.org/toc/2045-2322Abstract Recent studies have shown that transforming microglia phenotype from pro-inflammation of M1 phenotype to anti-inflammation and tissue-repairing M2 phenotype may be an effective therapeutic strategy for preventing ischemic stroke brain injury. Isosteviol Sodium (STV-Na) has shown promise as a neuroprotective agent in cerebral ischemia model, although its effect on microglial polarization and chronic recovery after stroke is not clear. Here, we demonstrated that STV-Na treatment significantly reduced cerebral ischemic damage at both acute and chronic time points. STV-Na has a profound regulatory effect on microglia response after stroke. It can promote M2 polarization and inhibit microglia-mediated inflammation (M1) response following stroke in vivo and in vitro. Furthermore, we also found that Growth Arrest-Specific 5 (GAS5) altered OGD/R-induced microglial activation by increasing Notch1 expression via miR-146a-5p, the mRNA level of GAS5 and the protein level of Notch1 in vivo and in vitro, were discovered that both downgraded with STV-Na. Taken together, the present study demonstrated that STV-Na exerted neuroprotective effects by modulating microglia/macrophage polarization in ischemic stroke via the GAS5/miR-146a-5p sponge. These findings provide new evidence that targeting STV-Na could be a treatment for the prevention of stroke-related brain damage.Hao ZhangMinyi LuXiaofeng ZhangYihe KuaiYing MeiQiwen TanKailun ZhongXiaoou SunWen TanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Zhang Minyi Lu Xiaofeng Zhang Yihe Kuai Ying Mei Qiwen Tan Kailun Zhong Xiaoou Sun Wen Tan Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
description	Abstract Recent studies have shown that transforming microglia phenotype from pro-inflammation of M1 phenotype to anti-inflammation and tissue-repairing M2 phenotype may be an effective therapeutic strategy for preventing ischemic stroke brain injury. Isosteviol Sodium (STV-Na) has shown promise as a neuroprotective agent in cerebral ischemia model, although its effect on microglial polarization and chronic recovery after stroke is not clear. Here, we demonstrated that STV-Na treatment significantly reduced cerebral ischemic damage at both acute and chronic time points. STV-Na has a profound regulatory effect on microglia response after stroke. It can promote M2 polarization and inhibit microglia-mediated inflammation (M1) response following stroke in vivo and in vitro. Furthermore, we also found that Growth Arrest-Specific 5 (GAS5) altered OGD/R-induced microglial activation by increasing Notch1 expression via miR-146a-5p, the mRNA level of GAS5 and the protein level of Notch1 in vivo and in vitro, were discovered that both downgraded with STV-Na. Taken together, the present study demonstrated that STV-Na exerted neuroprotective effects by modulating microglia/macrophage polarization in ischemic stroke via the GAS5/miR-146a-5p sponge. These findings provide new evidence that targeting STV-Na could be a treatment for the prevention of stroke-related brain damage.
format	article
author	Hao Zhang Minyi Lu Xiaofeng Zhang Yihe Kuai Ying Mei Qiwen Tan Kailun Zhong Xiaoou Sun Wen Tan
author_facet	Hao Zhang Minyi Lu Xiaofeng Zhang Yihe Kuai Ying Mei Qiwen Tan Kailun Zhong Xiaoou Sun Wen Tan
author_sort	Hao Zhang
title	Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
title_short	Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
title_full	Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
title_fullStr	Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
title_full_unstemmed	Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge
title_sort	isosteviol sodium protects against ischemic stroke by modulating microglia/macrophage polarization via disruption of gas5/mir-146a-5p sponge
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/234276ff02a14fe88e368e0c70c27f0a
work_keys_str_mv	AT haozhang isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT minyilu isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT xiaofengzhang isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT yihekuai isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT yingmei isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT qiwentan isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT kailunzhong isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT xiaoousun isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge AT wentan isosteviolsodiumprotectsagainstischemicstrokebymodulatingmicrogliamacrophagepolarizationviadisruptionofgas5mir146a5psponge
_version_	1718387988137771008

Isosteviol Sodium Protects against Ischemic Stroke by Modulating Microglia/Macrophage Polarization via Disruption of GAS5/miR-146a-5p sponge

Ejemplares similares