Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland

A retrospective examining of cytology specimens obtained and verified by a fine-needle aspiration biopsy from patients with autoimmune thyroiditis and benign thyroid gland (cyst and goiter) formations allowed to note that in thyroid lobes they coincided in various combinations, thus rising a questio...

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Autores principales:	A. V. Terletsky, L. G. Akhmerova, E. V. Evtushenko
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	autoimmune thyroiditis cyst nodular goiter hemosporidian infection Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2350c3b0ea16469c834aa0225b9294ee
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spelling	oai:doaj.org-article:2350c3b0ea16469c834aa0225b9294ee2021-11-22T07:09:52ZBlood parasite infection causing inflammatory reactions and benign formations in human thyroid gland2220-76192313-739810.15789/2220-7619-2019-1-155-161https://doaj.org/article/2350c3b0ea16469c834aa0225b9294ee2019-05-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/658https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398A retrospective examining of cytology specimens obtained and verified by a fine-needle aspiration biopsy from patients with autoimmune thyroiditis and benign thyroid gland (cyst and goiter) formations allowed to note that in thyroid lobes they coincided in various combinations, thus rising a question about their potential etiological relation. In particular, a hemosporidian (blood parasitic) infection was found while analyzing cytology specimens from patients with autoimmune thyroiditis and benign thyroid gland (cyst and goiter) tumors prestained by Romanowsky-Giemsa dye. An evolution of developing intra-thyrocyte hemosporidia was tracked during a long-term detailed analysis of cytology specimens noted above. A panel of select specimens was stained (re-stained) with Schiff reagent according to the Feulgen method to clarify position of thyrocyte DNA and hemosporidian pathogens. Owing to an absorption approach, Romanovsky-Giemsa method allowed to repeatedly use specimens pre-stained with Schiff reagent according to the Feulgen method, wherein fuchsine was incorporated into DNA molecules after they were hydrolyzed by hydrochloric acid to stain specimens into magenta-lilac color. It allowed to identify a parasitic DNA inside developing hemosporidia most probably at exoerythrocytic stage and some erythrocytes cyst-based medusiform structures. Such technique used to stain specimens from patients with autoimmune thyroiditis allowed to localize the thyrocyte nuclear DNA as well as punctate and diffuse cytoplasmic inclusions of parasitic DNA, including magenta-lilac nuclei of different sizes inside erythrocytes. Thyrocyte nuclear DNA as well as punctate and diffuse hemosporidian DNA were distinguished in nodular goiter. Moreover, hemosporidian DNA was identified in a form of magenta-lilac multi-size nuclei inside erythrocytes. In contrast, unstained hemosporidian protoplasm was revealed as light-colored band around erythrocyte nuclei. The intra-erythrocyte nuclear hemosporidian material of different sizes may evidence about various species and/or pathogen generations. Intra-thyrocyte development of hemosporidian infection in patients with goiter results in marked cytoplasmic hyperplasia and its vacuolization associated with thyrocyte nuclear deformation, vacuolization, decreased size and degradation (with highly probability of mutations and deletions), reaching a pre-neoplastic level.A. V. TerletskyL. G. AkhmerovaE. V. EvtushenkoSankt-Peterburg : NIIÈM imeni Pasteraarticleautoimmune thyroiditiscystnodular goiterhemosporidian infectionInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 1, Pp 155-161 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	autoimmune thyroiditis cyst nodular goiter hemosporidian infection Infectious and parasitic diseases RC109-216
spellingShingle	autoimmune thyroiditis cyst nodular goiter hemosporidian infection Infectious and parasitic diseases RC109-216 A. V. Terletsky L. G. Akhmerova E. V. Evtushenko Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
description	A retrospective examining of cytology specimens obtained and verified by a fine-needle aspiration biopsy from patients with autoimmune thyroiditis and benign thyroid gland (cyst and goiter) formations allowed to note that in thyroid lobes they coincided in various combinations, thus rising a question about their potential etiological relation. In particular, a hemosporidian (blood parasitic) infection was found while analyzing cytology specimens from patients with autoimmune thyroiditis and benign thyroid gland (cyst and goiter) tumors prestained by Romanowsky-Giemsa dye. An evolution of developing intra-thyrocyte hemosporidia was tracked during a long-term detailed analysis of cytology specimens noted above. A panel of select specimens was stained (re-stained) with Schiff reagent according to the Feulgen method to clarify position of thyrocyte DNA and hemosporidian pathogens. Owing to an absorption approach, Romanovsky-Giemsa method allowed to repeatedly use specimens pre-stained with Schiff reagent according to the Feulgen method, wherein fuchsine was incorporated into DNA molecules after they were hydrolyzed by hydrochloric acid to stain specimens into magenta-lilac color. It allowed to identify a parasitic DNA inside developing hemosporidia most probably at exoerythrocytic stage and some erythrocytes cyst-based medusiform structures. Such technique used to stain specimens from patients with autoimmune thyroiditis allowed to localize the thyrocyte nuclear DNA as well as punctate and diffuse cytoplasmic inclusions of parasitic DNA, including magenta-lilac nuclei of different sizes inside erythrocytes. Thyrocyte nuclear DNA as well as punctate and diffuse hemosporidian DNA were distinguished in nodular goiter. Moreover, hemosporidian DNA was identified in a form of magenta-lilac multi-size nuclei inside erythrocytes. In contrast, unstained hemosporidian protoplasm was revealed as light-colored band around erythrocyte nuclei. The intra-erythrocyte nuclear hemosporidian material of different sizes may evidence about various species and/or pathogen generations. Intra-thyrocyte development of hemosporidian infection in patients with goiter results in marked cytoplasmic hyperplasia and its vacuolization associated with thyrocyte nuclear deformation, vacuolization, decreased size and degradation (with highly probability of mutations and deletions), reaching a pre-neoplastic level.
format	article
author	A. V. Terletsky L. G. Akhmerova E. V. Evtushenko
author_facet	A. V. Terletsky L. G. Akhmerova E. V. Evtushenko
author_sort	A. V. Terletsky
title	Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
title_short	Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
title_full	Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
title_fullStr	Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
title_full_unstemmed	Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
title_sort	blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/2350c3b0ea16469c834aa0225b9294ee
work_keys_str_mv	AT avterletsky bloodparasiteinfectioncausinginflammatoryreactionsandbenignformationsinhumanthyroidgland AT lgakhmerova bloodparasiteinfectioncausinginflammatoryreactionsandbenignformationsinhumanthyroidgland AT evevtushenko bloodparasiteinfectioncausinginflammatoryreactionsandbenignformationsinhumanthyroidgland
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Blood parasite infection causing inflammatory reactions and benign formations in human thyroid gland

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