A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences

Abstract Coffee berry borer—CBB (Hypothenemus hampei) is a globally important economic pest of coffee (Coffea spp.). Despite current insect control methods for managing CBB, development of future control strategies requires a better understanding of its biology and interaction with its host plant. T...

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Autores principales:	Lucio Navarro-Escalante, Erick M. Hernandez-Hernandez, Jonathan Nuñez, Flor E. Acevedo, Alejandro Berrio, Luis M. Constantino, Beatriz E. Padilla-Hurtado, Diana Molina, Carmenza Gongora, Ricardo Acuña, Jeff Stuart, Pablo Benavides
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2356e79fe4ea4feb90c93f4c74f1501a
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spelling	oai:doaj.org-article:2356e79fe4ea4feb90c93f4c74f1501a2021-12-02T13:20:12ZA coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences10.1038/s41598-021-84068-12045-2322https://doaj.org/article/2356e79fe4ea4feb90c93f4c74f1501a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84068-1https://doaj.org/toc/2045-2322Abstract Coffee berry borer—CBB (Hypothenemus hampei) is a globally important economic pest of coffee (Coffea spp.). Despite current insect control methods for managing CBB, development of future control strategies requires a better understanding of its biology and interaction with its host plant. Towards this objective, we performed de novo CBB genome and transcriptome sequencing, improved CBB genome assembly and predicted 18,765 protein-encoding genes. Using genome and transcriptome data, we annotated the genes associated with chemosensation and found a reduced gene repertoire composed by 67 odorant receptors (ORs), 62 gustatory receptors (GRs), 33 ionotropic receptors (IRs) and 29 odorant-binding proteins (OBPs). In silico transcript abundance analysis of these chemosensory genes revealed expression enrichment in CBB adults compared with larva. Detection of differentially expressed chemosensory genes between males and females is likely associated with differences in host-finding behavior between sexes. Additionally, we discovered male-specific genome content and identified candidate male-specific expressed genes on these scaffolds, suggesting that a Y-like chromosome may be involved in the CBB’s functional haplodiploid mechanism of sex determination.Lucio Navarro-EscalanteErick M. Hernandez-HernandezJonathan NuñezFlor E. AcevedoAlejandro BerrioLuis M. ConstantinoBeatriz E. Padilla-HurtadoDiana MolinaCarmenza GongoraRicardo AcuñaJeff StuartPablo BenavidesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucio Navarro-Escalante Erick M. Hernandez-Hernandez Jonathan Nuñez Flor E. Acevedo Alejandro Berrio Luis M. Constantino Beatriz E. Padilla-Hurtado Diana Molina Carmenza Gongora Ricardo Acuña Jeff Stuart Pablo Benavides A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
description	Abstract Coffee berry borer—CBB (Hypothenemus hampei) is a globally important economic pest of coffee (Coffea spp.). Despite current insect control methods for managing CBB, development of future control strategies requires a better understanding of its biology and interaction with its host plant. Towards this objective, we performed de novo CBB genome and transcriptome sequencing, improved CBB genome assembly and predicted 18,765 protein-encoding genes. Using genome and transcriptome data, we annotated the genes associated with chemosensation and found a reduced gene repertoire composed by 67 odorant receptors (ORs), 62 gustatory receptors (GRs), 33 ionotropic receptors (IRs) and 29 odorant-binding proteins (OBPs). In silico transcript abundance analysis of these chemosensory genes revealed expression enrichment in CBB adults compared with larva. Detection of differentially expressed chemosensory genes between males and females is likely associated with differences in host-finding behavior between sexes. Additionally, we discovered male-specific genome content and identified candidate male-specific expressed genes on these scaffolds, suggesting that a Y-like chromosome may be involved in the CBB’s functional haplodiploid mechanism of sex determination.
format	article
author	Lucio Navarro-Escalante Erick M. Hernandez-Hernandez Jonathan Nuñez Flor E. Acevedo Alejandro Berrio Luis M. Constantino Beatriz E. Padilla-Hurtado Diana Molina Carmenza Gongora Ricardo Acuña Jeff Stuart Pablo Benavides
author_facet	Lucio Navarro-Escalante Erick M. Hernandez-Hernandez Jonathan Nuñez Flor E. Acevedo Alejandro Berrio Luis M. Constantino Beatriz E. Padilla-Hurtado Diana Molina Carmenza Gongora Ricardo Acuña Jeff Stuart Pablo Benavides
author_sort	Lucio Navarro-Escalante
title	A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
title_short	A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
title_full	A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
title_fullStr	A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
title_full_unstemmed	A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
title_sort	coffee berry borer (hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2356e79fe4ea4feb90c93f4c74f1501a
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A coffee berry borer (Hypothenemus hampei) genome assembly reveals a reduced chemosensory receptor gene repertoire and male-specific genome sequences

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