Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.

Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether...

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Autores principales:	Csilla Krausz, Juan Sandoval, Sergi Sayols, Chiara Chianese, Claudia Giachini, Holger Heyn, Manel Esteller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2360321a659c4e2c8535dc9cafaa1380
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spelling	oai:doaj.org-article:2360321a659c4e2c8535dc9cafaa13802021-11-18T08:13:26ZNovel insights into DNA methylation features in spermatozoa: stability and peculiarities.1932-620310.1371/journal.pone.0044479https://doaj.org/article/2360321a659c4e2c8535dc9cafaa13802012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23071498/?tool=EBIhttps://doaj.org/toc/1932-6203Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether it is stable among different normozoospermic individuals is still missing. The definition of the DNA methylation profile of normozoospermic men, the entity of inter-individual variability and the epigenetic characterization of quality-fractioned sperm subpopulations in the same subject (intra-individual variability) are relevant for a better understanding of pathological conditions. We addressed these questions by using the high resolution Infinium 450K methylation array and compared normal sperm DNA methylomes against somatic and cancer cells. Our study, based on the largest number of subjects (n = 8) ever considered for such a large number of CpGs (n = 487,517), provided clear evidence for i) a highly conserved DNA methylation profile among normozoospermic subjects; ii) a stable sperm DNA methylation pattern in different quality-fractioned sperm populations of the same individual. The latter finding is particularly relevant if we consider that different quality fractioned sperm subpopulations show differences in their structural features, metabolic and genomic profiles. We demonstrate, for the first time, that DNA methylation in normozoospermic men remains highly uniform regardless the quality of sperm subpopulations. In addition, our analysis provided both confirmatory and novel data concerning the sperm DNA methylome, including its peculiar features in respect to somatic and cancer cells. Our description about a highly polarized sperm DNA methylation profile, the clearly distinct genomic and functional organization of hypo- versus hypermethylated loci as well as the association of histone-enriched hypomethylated loci with embryonic development, which we now extended also to hypomethylated piRNAs-linked genes, provides solid basis for future basic and clinical research.Csilla KrauszJuan SandovalSergi SayolsChiara ChianeseClaudia GiachiniHolger HeynManel EstellerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e44479 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Csilla Krausz Juan Sandoval Sergi Sayols Chiara Chianese Claudia Giachini Holger Heyn Manel Esteller Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
description	Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether it is stable among different normozoospermic individuals is still missing. The definition of the DNA methylation profile of normozoospermic men, the entity of inter-individual variability and the epigenetic characterization of quality-fractioned sperm subpopulations in the same subject (intra-individual variability) are relevant for a better understanding of pathological conditions. We addressed these questions by using the high resolution Infinium 450K methylation array and compared normal sperm DNA methylomes against somatic and cancer cells. Our study, based on the largest number of subjects (n = 8) ever considered for such a large number of CpGs (n = 487,517), provided clear evidence for i) a highly conserved DNA methylation profile among normozoospermic subjects; ii) a stable sperm DNA methylation pattern in different quality-fractioned sperm populations of the same individual. The latter finding is particularly relevant if we consider that different quality fractioned sperm subpopulations show differences in their structural features, metabolic and genomic profiles. We demonstrate, for the first time, that DNA methylation in normozoospermic men remains highly uniform regardless the quality of sperm subpopulations. In addition, our analysis provided both confirmatory and novel data concerning the sperm DNA methylome, including its peculiar features in respect to somatic and cancer cells. Our description about a highly polarized sperm DNA methylation profile, the clearly distinct genomic and functional organization of hypo- versus hypermethylated loci as well as the association of histone-enriched hypomethylated loci with embryonic development, which we now extended also to hypomethylated piRNAs-linked genes, provides solid basis for future basic and clinical research.
format	article
author	Csilla Krausz Juan Sandoval Sergi Sayols Chiara Chianese Claudia Giachini Holger Heyn Manel Esteller
author_facet	Csilla Krausz Juan Sandoval Sergi Sayols Chiara Chianese Claudia Giachini Holger Heyn Manel Esteller
author_sort	Csilla Krausz
title	Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
title_short	Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
title_full	Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
title_fullStr	Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
title_full_unstemmed	Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.
title_sort	novel insights into dna methylation features in spermatozoa: stability and peculiarities.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2360321a659c4e2c8535dc9cafaa1380
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Novel insights into DNA methylation features in spermatozoa: stability and peculiarities.

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