Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)

<b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixatio...

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Autores principales:	Ole Jung, Bernhard Hesse, Sanja Stojanovic, Christian Seim, Timm Weitkamp, Milijana Batinic, Oliver Goerke, Željka Perić Kačarević, Patrick Rider, Stevo Najman, Mike Barbeck
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	guided bone regeneration (GBR) barrier membrane fixation screw pin biodegradation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2364fcc73cd44ba18d40530fc8ae319e
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spelling	oai:doaj.org-article:2364fcc73cd44ba18d40530fc8ae319e2021-11-25T17:57:58ZBiocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)10.3390/ijms2222125671422-00671661-6596https://doaj.org/article/2364fcc73cd44ba18d40530fc8ae319e2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12567https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixation screws for maxillofacial and dental surgical applications were investigated in vitro and in vivo to evaluate the biocompatibility of the HF coating. <b>Methods:</b> Mg alloy screws that had either undergone a surface treatment with hydrofluoric-acid (HF) or left untreated were investigated. In vitro investigation included XTT, BrdU and LDH in accordance with the DIN ISO 10993-5/-12. In vivo, the screws were implanted into the tibia of rabbits. After 3 and 6 weeks, degradation, local tissue reactions and bony integration were analyzed histopathologically and histomorphometrically. Additionally, SEM/EDX analysis and synchrotron phase-contrast microtomography (µCT) measurements were conducted. The in vitro analyses revealed that the Mg screws are cytocompatible, with improved results when the surface had been passivated with HF. In vivo, the HF-treated Mg screws implanted showed a reduction in gas formation, slower biodegradation and a better bony integration in comparison to the untreated Mg screws. Histopathologically, the HF-passivated screws induced a layer of macrophages as part of its biodegradation process, whereas the untreated screws caused a slight fibrous tissue reaction. SEM/EDX analysis showed that both screws formed a similar layer of calcium phosphates on their surfaces and were surrounded by bone. Furthermore, the µCT revealed the presence of a metallic core of the screws, a faster absorbing corrosion front and a slow absorbing region of corroded magnesium. <b>Conclusions:</b> Overall, the HF-passivated Mg fixation screws showed significantly better biocompatibility in vitro and in vivo compared to the untreated screws.Ole JungBernhard HesseSanja StojanovicChristian SeimTimm WeitkampMilijana BatinicOliver GoerkeŽeljka Perić KačarevićPatrick RiderStevo NajmanMike BarbeckMDPI AGarticleguided bone regeneration (GBR)barrier membranefixationscrewpinbiodegradationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12567, p 12567 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	guided bone regeneration (GBR) barrier membrane fixation screw pin biodegradation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	guided bone regeneration (GBR) barrier membrane fixation screw pin biodegradation Biology (General) QH301-705.5 Chemistry QD1-999 Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
description	<b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixation screws for maxillofacial and dental surgical applications were investigated in vitro and in vivo to evaluate the biocompatibility of the HF coating. <b>Methods:</b> Mg alloy screws that had either undergone a surface treatment with hydrofluoric-acid (HF) or left untreated were investigated. In vitro investigation included XTT, BrdU and LDH in accordance with the DIN ISO 10993-5/-12. In vivo, the screws were implanted into the tibia of rabbits. After 3 and 6 weeks, degradation, local tissue reactions and bony integration were analyzed histopathologically and histomorphometrically. Additionally, SEM/EDX analysis and synchrotron phase-contrast microtomography (µCT) measurements were conducted. The in vitro analyses revealed that the Mg screws are cytocompatible, with improved results when the surface had been passivated with HF. In vivo, the HF-treated Mg screws implanted showed a reduction in gas formation, slower biodegradation and a better bony integration in comparison to the untreated Mg screws. Histopathologically, the HF-passivated screws induced a layer of macrophages as part of its biodegradation process, whereas the untreated screws caused a slight fibrous tissue reaction. SEM/EDX analysis showed that both screws formed a similar layer of calcium phosphates on their surfaces and were surrounded by bone. Furthermore, the µCT revealed the presence of a metallic core of the screws, a faster absorbing corrosion front and a slow absorbing region of corroded magnesium. <b>Conclusions:</b> Overall, the HF-passivated Mg fixation screws showed significantly better biocompatibility in vitro and in vivo compared to the untreated screws.
format	article
author	Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck
author_facet	Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck
author_sort	Ole Jung
title	Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
title_short	Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
title_full	Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
title_fullStr	Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
title_full_unstemmed	Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
title_sort	biocompatibility analyses of hf-passivated magnesium screws for guided bone regeneration (gbr)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2364fcc73cd44ba18d40530fc8ae319e
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Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)

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