A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold
Abstract One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. In the present study, polycaprolactone/gelatin...
Guardado en:
Autores principales: | , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
|
Materias: | |
Acceso en línea: | https://doaj.org/article/236d3ae0ef1b410b93a076de5c1c66e9 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:236d3ae0ef1b410b93a076de5c1c66e9 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:236d3ae0ef1b410b93a076de5c1c66e92021-12-02T14:22:00ZA Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold10.1038/s41598-020-59931-22045-2322https://doaj.org/article/236d3ae0ef1b410b93a076de5c1c66e92020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-59931-2https://doaj.org/toc/2045-2322Abstract One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. In the present study, polycaprolactone/gelatin (PCL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (PU/EEP). The PU/EEP membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells’ adhesion and proliferation. The bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers’ diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats’ skin wound model. Taking together, the PU/EEP-PCL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities.Asghar EskandariniaAmirhosein KefayatMaria AghebMohammad RafieniaMoloud Amini BaghbadoraniSepehr NavidKarim EbrahimpourDarioush KhodabakhshiFatemeh GhahremaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Asghar Eskandarinia Amirhosein Kefayat Maria Agheb Mohammad Rafienia Moloud Amini Baghbadorani Sepehr Navid Karim Ebrahimpour Darioush Khodabakhshi Fatemeh Ghahremani A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
description |
Abstract One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. In the present study, polycaprolactone/gelatin (PCL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (PU/EEP). The PU/EEP membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells’ adhesion and proliferation. The bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers’ diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats’ skin wound model. Taking together, the PU/EEP-PCL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities. |
format |
article |
author |
Asghar Eskandarinia Amirhosein Kefayat Maria Agheb Mohammad Rafienia Moloud Amini Baghbadorani Sepehr Navid Karim Ebrahimpour Darioush Khodabakhshi Fatemeh Ghahremani |
author_facet |
Asghar Eskandarinia Amirhosein Kefayat Maria Agheb Mohammad Rafienia Moloud Amini Baghbadorani Sepehr Navid Karim Ebrahimpour Darioush Khodabakhshi Fatemeh Ghahremani |
author_sort |
Asghar Eskandarinia |
title |
A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
title_short |
A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
title_full |
A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
title_fullStr |
A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
title_full_unstemmed |
A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold |
title_sort |
novel bilayer wound dressing composed of a dense polyurethane/propolis membrane and a biodegradable polycaprolactone/gelatin nanofibrous scaffold |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/236d3ae0ef1b410b93a076de5c1c66e9 |
work_keys_str_mv |
AT asghareskandarinia anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT amirhoseinkefayat anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT mariaagheb anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT mohammadrafienia anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT moloudaminibaghbadorani anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT sepehrnavid anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT karimebrahimpour anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT darioushkhodabakhshi anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT fatemehghahremani anovelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT asghareskandarinia novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT amirhoseinkefayat novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT mariaagheb novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT mohammadrafienia novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT moloudaminibaghbadorani novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT sepehrnavid novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT karimebrahimpour novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT darioushkhodabakhshi novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold AT fatemehghahremani novelbilayerwounddressingcomposedofadensepolyurethanepropolismembraneandabiodegradablepolycaprolactonegelatinnanofibrousscaffold |
_version_ |
1718391473581326336 |