Lysozyme-like Protein Produced by Bifidobacterium longum Regulates Human Gut Microbiota Using In Vitro Models

The extracellular secreted protein of Bifidobacterium longum (B. longum) plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial...

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Autores principales:	Mingzhu Du, Xinqiang Xie, Shuanghong Yang, Ying Li, Tong Jiang, Juan Yang, Longyan Li, Yunxiao Huang, Qingping Wu, Wei Chen, Jumei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bifidobacterium longum</i> lysozyme-like protein bioinformatics bioactivity microbiome balance Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/2371725cf3e843c5b230cd6c41c1dc6b
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spelling	oai:doaj.org-article:2371725cf3e843c5b230cd6c41c1dc6b2021-11-11T18:29:33ZLysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models10.3390/molecules262164801420-3049https://doaj.org/article/2371725cf3e843c5b230cd6c41c1dc6b2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6480https://doaj.org/toc/1420-3049The extracellular secreted protein of <i>Bifidobacterium longum</i> (<i>B. longum</i>) plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial peptide. In this study, the amino acid sequence of a lysozyme-like protein of <i>B. longum</i> based on whole-genome data of an isolate from human gut feces was found. We further predicted functional domains from the amino acid sequence, purified the protein, and verified its bioactivity. The growth of some bacteria were significantly delayed by the 020402_LYZ M1 protein. In addition, the gut microbiota was analyzed via high-throughput sequencing of 16S rRNA genes and an in vitro fermentation model, and the fluctuations in the gut microbiota under the treatment of 020402_LYZ M1 protein were characterized. The 020402_LYZ M1 protein affected the composition of human gut microbiota significantly, implying that the protein is able to communicate with intestinal microbes as a regulatory factor.Mingzhu DuXinqiang XieShuanghong YangYing LiTong JiangJuan YangLongyan LiYunxiao HuangQingping WuWei ChenJumei ZhangMDPI AGarticle<i>Bifidobacterium longum</i>lysozyme-like proteinbioinformaticsbioactivitymicrobiomebalanceOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6480, p 6480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bifidobacterium longum</i> lysozyme-like protein bioinformatics bioactivity microbiome balance Organic chemistry QD241-441
spellingShingle	<i>Bifidobacterium longum</i> lysozyme-like protein bioinformatics bioactivity microbiome balance Organic chemistry QD241-441 Mingzhu Du Xinqiang Xie Shuanghong Yang Ying Li Tong Jiang Juan Yang Longyan Li Yunxiao Huang Qingping Wu Wei Chen Jumei Zhang Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
description	The extracellular secreted protein of <i>Bifidobacterium longum</i> (<i>B. longum</i>) plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial peptide. In this study, the amino acid sequence of a lysozyme-like protein of <i>B. longum</i> based on whole-genome data of an isolate from human gut feces was found. We further predicted functional domains from the amino acid sequence, purified the protein, and verified its bioactivity. The growth of some bacteria were significantly delayed by the 020402_LYZ M1 protein. In addition, the gut microbiota was analyzed via high-throughput sequencing of 16S rRNA genes and an in vitro fermentation model, and the fluctuations in the gut microbiota under the treatment of 020402_LYZ M1 protein were characterized. The 020402_LYZ M1 protein affected the composition of human gut microbiota significantly, implying that the protein is able to communicate with intestinal microbes as a regulatory factor.
format	article
author	Mingzhu Du Xinqiang Xie Shuanghong Yang Ying Li Tong Jiang Juan Yang Longyan Li Yunxiao Huang Qingping Wu Wei Chen Jumei Zhang
author_facet	Mingzhu Du Xinqiang Xie Shuanghong Yang Ying Li Tong Jiang Juan Yang Longyan Li Yunxiao Huang Qingping Wu Wei Chen Jumei Zhang
author_sort	Mingzhu Du
title	Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
title_short	Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
title_full	Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
title_fullStr	Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
title_full_unstemmed	Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models
title_sort	lysozyme-like protein produced by <i>bifidobacterium longum</i> regulates human gut microbiota using in vitro models
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2371725cf3e843c5b230cd6c41c1dc6b
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Lysozyme-like Protein Produced by <i>Bifidobacterium longum</i> Regulates Human Gut Microbiota Using In Vitro Models

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