Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects

Molecular chaperones, many of which are heat shock proteins, play a role in cell stress response and regulate the immune system in various ways, such as in inflammatory/autoimmune reactions. It would be interesting to study the involvement of these molecules in the damage done to COVID-19-infected l...

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Autores principales:	Rosario Barone, Antonella Marino Gammazza, Letizia Paladino, Alessandro Pitruzzella, Giulio Spinoso, Monica Salerno, Francesco Sessa, Cristoforo Pomara, Francesco Cappello, Francesca Rappa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 COVID-19 Hsp60 Hsp90 endothelium inflammation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2375bd6775c047169fe43a1316b814bb
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spelling	oai:doaj.org-article:2375bd6775c047169fe43a1316b814bb2021-11-25T17:11:51ZMorphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects10.3390/cells101131362073-4409https://doaj.org/article/2375bd6775c047169fe43a1316b814bb2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3136https://doaj.org/toc/2073-4409Molecular chaperones, many of which are heat shock proteins, play a role in cell stress response and regulate the immune system in various ways, such as in inflammatory/autoimmune reactions. It would be interesting to study the involvement of these molecules in the damage done to COVID-19-infected lungs. In our study, we performed a histological analysis and an immunomorphological evaluation on lung samples from subjects who succumbed to COVID-19 and subjects who died from other causes. We also assessed Hsp60 and Hsp90 distribution in lung samples to determine their location and post-translational modifications. We found histological alterations that could be considered pathognomonic for COVID-19-related lung disease. Hsp60 and Hsp90 immunopositivity was significantly higher in the COVID-19 group compared to the controls, and immunolocalization was in the plasma membrane of the endothelial cells in COVID-19 subjects. The colocalization ratios for Hsp60/3-nitrotyrosine and Hsp60/acetylate-lisine were significantly increased in the COVID-19 group compared to the control group, similar to the colocalization ratio for Hsp90/acetylate-lisine. The histological and immunohistochemical findings led us to hypothesize that Hsp60 and Hsp90 might have a role in the onset of the thromboembolic phenomena that lead to death in a limited number of subjects affected by COVID-19. Further studies on a larger number of samples obtained from autopsies would allow to confirm these data as well as discover new biomarkers useful in the battle against this disease.Rosario BaroneAntonella Marino GammazzaLetizia PaladinoAlessandro PitruzzellaGiulio SpinosoMonica SalernoFrancesco SessaCristoforo PomaraFrancesco CappelloFrancesca RappaMDPI AGarticleSARS-CoV-2COVID-19Hsp60Hsp90endotheliuminflammationBiology (General)QH301-705.5ENCells, Vol 10, Iss 3136, p 3136 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 COVID-19 Hsp60 Hsp90 endothelium inflammation Biology (General) QH301-705.5
spellingShingle	SARS-CoV-2 COVID-19 Hsp60 Hsp90 endothelium inflammation Biology (General) QH301-705.5 Rosario Barone Antonella Marino Gammazza Letizia Paladino Alessandro Pitruzzella Giulio Spinoso Monica Salerno Francesco Sessa Cristoforo Pomara Francesco Cappello Francesca Rappa Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
description	Molecular chaperones, many of which are heat shock proteins, play a role in cell stress response and regulate the immune system in various ways, such as in inflammatory/autoimmune reactions. It would be interesting to study the involvement of these molecules in the damage done to COVID-19-infected lungs. In our study, we performed a histological analysis and an immunomorphological evaluation on lung samples from subjects who succumbed to COVID-19 and subjects who died from other causes. We also assessed Hsp60 and Hsp90 distribution in lung samples to determine their location and post-translational modifications. We found histological alterations that could be considered pathognomonic for COVID-19-related lung disease. Hsp60 and Hsp90 immunopositivity was significantly higher in the COVID-19 group compared to the controls, and immunolocalization was in the plasma membrane of the endothelial cells in COVID-19 subjects. The colocalization ratios for Hsp60/3-nitrotyrosine and Hsp60/acetylate-lisine were significantly increased in the COVID-19 group compared to the control group, similar to the colocalization ratio for Hsp90/acetylate-lisine. The histological and immunohistochemical findings led us to hypothesize that Hsp60 and Hsp90 might have a role in the onset of the thromboembolic phenomena that lead to death in a limited number of subjects affected by COVID-19. Further studies on a larger number of samples obtained from autopsies would allow to confirm these data as well as discover new biomarkers useful in the battle against this disease.
format	article
author	Rosario Barone Antonella Marino Gammazza Letizia Paladino Alessandro Pitruzzella Giulio Spinoso Monica Salerno Francesco Sessa Cristoforo Pomara Francesco Cappello Francesca Rappa
author_facet	Rosario Barone Antonella Marino Gammazza Letizia Paladino Alessandro Pitruzzella Giulio Spinoso Monica Salerno Francesco Sessa Cristoforo Pomara Francesco Cappello Francesca Rappa
author_sort	Rosario Barone
title	Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
title_short	Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
title_full	Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
title_fullStr	Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
title_full_unstemmed	Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects
title_sort	morphological alterations and stress protein variations in lung biopsies obtained from autopsies of covid-19 subjects
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2375bd6775c047169fe43a1316b814bb
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Morphological Alterations and Stress Protein Variations in Lung Biopsies Obtained from Autopsies of COVID-19 Subjects

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