Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.

Bacteria live in an ever-changing environment and must alter protein expression promptly to adapt to these changes and survive. Specific response genes that are regulated by a subset of alternative σ(70)-like transcription factors have evolved in order to respond to this changing environment. Recent...

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Autores principales:	Robert Antonius Gerhardus Huis in 't Veld, Antonius Marcellinus Willemsen, Antonius Hubertus Cornelis van Kampen, Edward John Bradley, Frank Baas, Yvonne Pannekoek, Arie van der Ende
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2379ec04e1374f9b9cf578c8d01c3094
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spelling	oai:doaj.org-article:2379ec04e1374f9b9cf578c8d01c30942021-11-18T07:32:01ZDeep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.1932-620310.1371/journal.pone.0029002https://doaj.org/article/2379ec04e1374f9b9cf578c8d01c30942011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194974/?tool=EBIhttps://doaj.org/toc/1932-6203Bacteria live in an ever-changing environment and must alter protein expression promptly to adapt to these changes and survive. Specific response genes that are regulated by a subset of alternative σ(70)-like transcription factors have evolved in order to respond to this changing environment. Recently, we have described the existence of a σ(E) regulon including the anti-σ-factor MseR in the obligate human bacterial pathogen Neisseria meningitidis. To unravel the complete σ(E) regulon in N. meningitidis, we sequenced total RNA transcriptional content of wild type meningococci and compared it with that of mseR mutant cells (ΔmseR) in which σ(E) is highly expressed. Eleven coding genes and one non-coding gene were found to be differentially expressed between H44/76 wildtype and H44/76ΔmseR cells. Five of the 6 genes of the σ(E) operon, msrA/msrB, and the gene encoding a pepSY-associated TM helix family protein showed enhanced transcription, whilst aniA encoding a nitrite reductase and nspA encoding the vaccine candidate Neisserial surface protein A showed decreased transcription. Analysis of differential expression in IGRs showed enhanced transcription of a non-coding RNA molecule, identifying a σ(E) dependent small non-coding RNA. Together this constitutes the first complete exploration of an alternative σ-factor regulon in N. meningitidis. The results direct to a relatively small regulon indicative for a strictly defined response consistent with a relatively stable niche, the human throat, where N. meningitidis resides.Robert Antonius Gerhardus Huis in 't VeldAntonius Marcellinus WillemsenAntonius Hubertus Cornelis van KampenEdward John BradleyFrank BaasYvonne PannekoekArie van der EndePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29002 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robert Antonius Gerhardus Huis in 't Veld Antonius Marcellinus Willemsen Antonius Hubertus Cornelis van Kampen Edward John Bradley Frank Baas Yvonne Pannekoek Arie van der Ende Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
description	Bacteria live in an ever-changing environment and must alter protein expression promptly to adapt to these changes and survive. Specific response genes that are regulated by a subset of alternative σ(70)-like transcription factors have evolved in order to respond to this changing environment. Recently, we have described the existence of a σ(E) regulon including the anti-σ-factor MseR in the obligate human bacterial pathogen Neisseria meningitidis. To unravel the complete σ(E) regulon in N. meningitidis, we sequenced total RNA transcriptional content of wild type meningococci and compared it with that of mseR mutant cells (ΔmseR) in which σ(E) is highly expressed. Eleven coding genes and one non-coding gene were found to be differentially expressed between H44/76 wildtype and H44/76ΔmseR cells. Five of the 6 genes of the σ(E) operon, msrA/msrB, and the gene encoding a pepSY-associated TM helix family protein showed enhanced transcription, whilst aniA encoding a nitrite reductase and nspA encoding the vaccine candidate Neisserial surface protein A showed decreased transcription. Analysis of differential expression in IGRs showed enhanced transcription of a non-coding RNA molecule, identifying a σ(E) dependent small non-coding RNA. Together this constitutes the first complete exploration of an alternative σ-factor regulon in N. meningitidis. The results direct to a relatively small regulon indicative for a strictly defined response consistent with a relatively stable niche, the human throat, where N. meningitidis resides.
format	article
author	Robert Antonius Gerhardus Huis in 't Veld Antonius Marcellinus Willemsen Antonius Hubertus Cornelis van Kampen Edward John Bradley Frank Baas Yvonne Pannekoek Arie van der Ende
author_facet	Robert Antonius Gerhardus Huis in 't Veld Antonius Marcellinus Willemsen Antonius Hubertus Cornelis van Kampen Edward John Bradley Frank Baas Yvonne Pannekoek Arie van der Ende
author_sort	Robert Antonius Gerhardus Huis in 't Veld
title	Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
title_short	Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
title_full	Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
title_fullStr	Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
title_full_unstemmed	Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.
title_sort	deep sequencing whole transcriptome exploration of the σe regulon in neisseria meningitidis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2379ec04e1374f9b9cf578c8d01c3094
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Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.

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