Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.

Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carina S S Gomes-Santos, Joanna Braks, Miguel Prudêncio, Céline Carret, Ana Rita Gomes, Arnab Pain, Theresa Feltwell, Shahid Khan, Andrew Waters, Chris Janse, Gunnar R Mair, Maria M Mota
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2381a52cc7c946c5a4e87142f6b95c7f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2381a52cc7c946c5a4e87142f6b95c7f
record_format	dspace
spelling	oai:doaj.org-article:2381a52cc7c946c5a4e87142f6b95c7f2021-11-18T06:03:23ZTransition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.1553-73661553-737410.1371/journal.ppat.1002046https://doaj.org/article/2381a52cc7c946c5a4e87142f6b95c7f2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625527/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.Carina S S Gomes-SantosJoanna BraksMiguel PrudêncioCéline CarretAna Rita GomesArnab PainTheresa FeltwellShahid KhanAndrew WatersChris JanseGunnar R MairMaria M MotaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 5, p e1002046 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Carina S S Gomes-Santos Joanna Braks Miguel Prudêncio Céline Carret Ana Rita Gomes Arnab Pain Theresa Feltwell Shahid Khan Andrew Waters Chris Janse Gunnar R Mair Maria M Mota Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
description	Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.
format	article
author	Carina S S Gomes-Santos Joanna Braks Miguel Prudêncio Céline Carret Ana Rita Gomes Arnab Pain Theresa Feltwell Shahid Khan Andrew Waters Chris Janse Gunnar R Mair Maria M Mota
author_facet	Carina S S Gomes-Santos Joanna Braks Miguel Prudêncio Céline Carret Ana Rita Gomes Arnab Pain Theresa Feltwell Shahid Khan Andrew Waters Chris Janse Gunnar R Mair Maria M Mota
author_sort	Carina S S Gomes-Santos
title	Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
title_short	Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
title_full	Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
title_fullStr	Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
title_full_unstemmed	Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.
title_sort	transition of plasmodium sporozoites into liver stage-like forms is regulated by the rna binding protein pumilio.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2381a52cc7c946c5a4e87142f6b95c7f
work_keys_str_mv	AT carinassgomessantos transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT joannabraks transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT miguelprudencio transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT celinecarret transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT anaritagomes transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT arnabpain transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT theresafeltwell transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT shahidkhan transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT andrewwaters transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT chrisjanse transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT gunnarrmair transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio AT mariammota transitionofplasmodiumsporozoitesintoliverstagelikeformsisregulatedbythernabindingproteinpumilio
_version_	1718424654274625536

Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.

Ejemplares similares