Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method

Pharmacokinetics studies of domperidone generally analyze plasma matrix samples. The present work aimed to develop and validate a rapid and simple reversed phase-HPLC method for quantifying domperidone in plasma matrices. The chromatographic method implemented: 1. Luna Phenomenex® C18 (250 mm × 4.6...

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Autores principales:	Sekar Ayu Pawestri, Akhmad Kharis Nugroho, Endang Lukitaningsih, Purwantiningsih Purwantiningsih
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	domperidone rp-hplc spiked plasma validation Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2389c520e6f04a299f8a3bf9d0519868
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spelling	oai:doaj.org-article:2389c520e6f04a299f8a3bf9d05198682021-12-02T18:02:38ZValidation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method1411-94202460-157810.22146/ijc.64253https://doaj.org/article/2389c520e6f04a299f8a3bf9d05198682021-08-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/64253https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Pharmacokinetics studies of domperidone generally analyze plasma matrix samples. The present work aimed to develop and validate a rapid and simple reversed phase-HPLC method for quantifying domperidone in plasma matrices. The chromatographic method implemented: 1. Luna Phenomenex® C18 (250 mm × 4.6 mm i.d; 5 µm) column, 2. isocratic mobile phase mixture of phosphate buffer 0.02 M:acetonitrile (70:30, v/v) with a flow rate of 1 mL/min, 3. UV detection at 285 nm. Domperidone and propranolol hydrochloride (as internal standard) were extracted from the deproteinated plasma sample. The method linearity was 0.998 in the range concentration of 15–200 ng/mL. The percentage of accuracy error was between -8.49–4.31%, while the percentage coefficient variation of precision ranged between 5.11–14.24%. This proposed method was simple, rapid (separation time less than 10 min), and selective. The validation parameters responses satisfied the method's requirements to determine domperidone in a plasma sample.Sekar Ayu PawestriAkhmad Kharis NugrohoEndang LukitaningsihPurwantiningsih PurwantiningsihDepartment of Chemistry, Universitas Gadjah Madaarticledomperidonerp-hplcspiked plasmavalidationChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 5, Pp 1180-1186 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	domperidone rp-hplc spiked plasma validation Chemistry QD1-999
spellingShingle	domperidone rp-hplc spiked plasma validation Chemistry QD1-999 Sekar Ayu Pawestri Akhmad Kharis Nugroho Endang Lukitaningsih Purwantiningsih Purwantiningsih Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
description	Pharmacokinetics studies of domperidone generally analyze plasma matrix samples. The present work aimed to develop and validate a rapid and simple reversed phase-HPLC method for quantifying domperidone in plasma matrices. The chromatographic method implemented: 1. Luna Phenomenex® C18 (250 mm × 4.6 mm i.d; 5 µm) column, 2. isocratic mobile phase mixture of phosphate buffer 0.02 M:acetonitrile (70:30, v/v) with a flow rate of 1 mL/min, 3. UV detection at 285 nm. Domperidone and propranolol hydrochloride (as internal standard) were extracted from the deproteinated plasma sample. The method linearity was 0.998 in the range concentration of 15–200 ng/mL. The percentage of accuracy error was between -8.49–4.31%, while the percentage coefficient variation of precision ranged between 5.11–14.24%. This proposed method was simple, rapid (separation time less than 10 min), and selective. The validation parameters responses satisfied the method's requirements to determine domperidone in a plasma sample.
format	article
author	Sekar Ayu Pawestri Akhmad Kharis Nugroho Endang Lukitaningsih Purwantiningsih Purwantiningsih
author_facet	Sekar Ayu Pawestri Akhmad Kharis Nugroho Endang Lukitaningsih Purwantiningsih Purwantiningsih
author_sort	Sekar Ayu Pawestri
title	Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
title_short	Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
title_full	Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
title_fullStr	Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
title_full_unstemmed	Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method
title_sort	validation and quantification of domperidone in spiked plasma matrix using reversed phase hplc-uv method
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/2389c520e6f04a299f8a3bf9d0519868
work_keys_str_mv	AT sekarayupawestri validationandquantificationofdomperidoneinspikedplasmamatrixusingreversedphasehplcuvmethod AT akhmadkharisnugroho validationandquantificationofdomperidoneinspikedplasmamatrixusingreversedphasehplcuvmethod AT endanglukitaningsih validationandquantificationofdomperidoneinspikedplasmamatrixusingreversedphasehplcuvmethod AT purwantiningsihpurwantiningsih validationandquantificationofdomperidoneinspikedplasmamatrixusingreversedphasehplcuvmethod
_version_	1718378856770961408

Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method

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