Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Komagataeibacter spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pietro Cannazza, Antti J. Rissanen, Dieval Guizelini, Pauli Losoi, Essi Sarlin, Diego Romano, Ville Santala, Rahul Mangayil
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Komagataeibacter rhaeticus</i> bacterial cellulose crude glycerol minimal medium whole-genome analysis acetate Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/238a885180f84db1b2256af2b595ba0b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:238a885180f84db1b2256af2b595ba0b
record_format	dspace
spelling	oai:doaj.org-article:238a885180f84db1b2256af2b595ba0b2021-11-25T18:24:24ZCharacterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production10.3390/microorganisms91122302076-2607https://doaj.org/article/238a885180f84db1b2256af2b595ba0b2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2230https://doaj.org/toc/2076-2607<i>Komagataeibacter</i> spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of <i>Komagataeibacter</i> spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in <i>Komagataeibacter</i> spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to <i>Komagataeibacter</i> genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when <i>K. rhaeticus</i> ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.Pietro CannazzaAntti J. RissanenDieval GuizeliniPauli LosoiEssi SarlinDiego RomanoVille SantalaRahul MangayilMDPI AGarticle<i>Komagataeibacter rhaeticus</i>bacterial cellulosecrude glycerolminimal mediumwhole-genome analysisacetateBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2230, p 2230 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Komagataeibacter rhaeticus</i> bacterial cellulose crude glycerol minimal medium whole-genome analysis acetate Biology (General) QH301-705.5
spellingShingle	<i>Komagataeibacter rhaeticus</i> bacterial cellulose crude glycerol minimal medium whole-genome analysis acetate Biology (General) QH301-705.5 Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
description	<i>Komagataeibacter</i> spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of <i>Komagataeibacter</i> spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in <i>Komagataeibacter</i> spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to <i>Komagataeibacter</i> genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when <i>K. rhaeticus</i> ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.
format	article
author	Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil
author_facet	Pietro Cannazza Antti J. Rissanen Dieval Guizelini Pauli Losoi Essi Sarlin Diego Romano Ville Santala Rahul Mangayil
author_sort	Pietro Cannazza
title	Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
title_short	Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
title_full	Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
title_fullStr	Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
title_full_unstemmed	Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production
title_sort	characterization of <i>komagataeibacter</i> isolate reveals new prospects in waste stream valorization for bacterial cellulose production
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/238a885180f84db1b2256af2b595ba0b
work_keys_str_mv	AT pietrocannazza characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT anttijrissanen characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT dievalguizelini characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT paulilosoi characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT essisarlin characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT diegoromano characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT villesantala characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction AT rahulmangayil characterizationofikomagataeibacteriisolaterevealsnewprospectsinwastestreamvalorizationforbacterialcelluloseproduction
_version_	1718411183923396608

Characterization of <i>Komagataeibacter</i> Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Ejemplares similares