YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer

Abstract Y-box binding protein 2 (YBX2) has been associated with the properties of both germ cells and cancer cells. We hypothesized that YBX2 might contribute to the characteristics of cancer stem cells (CSCs). In this study, we clarified the function of YBX2 in endometrial cancer stem cells. We es...

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Autores principales:	Izumi Suzuki, Sachiko Yoshida, Kouichi Tabu, Soshi Kusunoki, Yumiko Matsumura, Hiroto Izumi, Kazuo Asanoma, Hiroshi Yagi, Ichiro Onoyama, Kenzo Sonoda, Kimitoshi Kohno, Tetsuya Taga, Atsuo Itakura, Satoru Takeda, Kiyoko Kato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2393a0674b334285888ea55f9ddad4b0
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spelling	oai:doaj.org-article:2393a0674b334285888ea55f9ddad4b02021-12-02T12:11:45ZYBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer10.1038/s41598-021-83200-52045-2322https://doaj.org/article/2393a0674b334285888ea55f9ddad4b02021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83200-5https://doaj.org/toc/2045-2322Abstract Y-box binding protein 2 (YBX2) has been associated with the properties of both germ cells and cancer cells. We hypothesized that YBX2 might contribute to the characteristics of cancer stem cells (CSCs). In this study, we clarified the function of YBX2 in endometrial cancer stem cells. We established a human YBX2-expressing Ishikawa (IK) cell line (IK-YBX2 cells). We analyzed gene expression associated with stemness and isolated SP cells from IK-YBX2 cells. The SP population of IK-YBX2 cells, the expression of ALDH1 and serial sphere-forming capacity were associated with levels of YBX2 expression. IK-YBX2 cells were resistant to anti-cancer drugs. In gene expression analysis, a gene for cancer testis antigen, CT45, was generally overexpressed in IK-YBX2 cells. YBX2-mediated CT45 expression was associated with increased levels of self-renewal capacity and paclitaxel resistance. The level of CT45 expression was enhanced in high-grade and/or advanced stages of human endometrial cancer tissues. We conclude that expression of YBX2 is essential for the stem cell-like phenotype. CT45 contributes to stemness associated with YBX2 and might be related to the progression of endometrial cancer.Izumi SuzukiSachiko YoshidaKouichi TabuSoshi KusunokiYumiko MatsumuraHiroto IzumiKazuo AsanomaHiroshi YagiIchiro OnoyamaKenzo SonodaKimitoshi KohnoTetsuya TagaAtsuo ItakuraSatoru TakedaKiyoko KatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Izumi Suzuki Sachiko Yoshida Kouichi Tabu Soshi Kusunoki Yumiko Matsumura Hiroto Izumi Kazuo Asanoma Hiroshi Yagi Ichiro Onoyama Kenzo Sonoda Kimitoshi Kohno Tetsuya Taga Atsuo Itakura Satoru Takeda Kiyoko Kato YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
description	Abstract Y-box binding protein 2 (YBX2) has been associated with the properties of both germ cells and cancer cells. We hypothesized that YBX2 might contribute to the characteristics of cancer stem cells (CSCs). In this study, we clarified the function of YBX2 in endometrial cancer stem cells. We established a human YBX2-expressing Ishikawa (IK) cell line (IK-YBX2 cells). We analyzed gene expression associated with stemness and isolated SP cells from IK-YBX2 cells. The SP population of IK-YBX2 cells, the expression of ALDH1 and serial sphere-forming capacity were associated with levels of YBX2 expression. IK-YBX2 cells were resistant to anti-cancer drugs. In gene expression analysis, a gene for cancer testis antigen, CT45, was generally overexpressed in IK-YBX2 cells. YBX2-mediated CT45 expression was associated with increased levels of self-renewal capacity and paclitaxel resistance. The level of CT45 expression was enhanced in high-grade and/or advanced stages of human endometrial cancer tissues. We conclude that expression of YBX2 is essential for the stem cell-like phenotype. CT45 contributes to stemness associated with YBX2 and might be related to the progression of endometrial cancer.
format	article
author	Izumi Suzuki Sachiko Yoshida Kouichi Tabu Soshi Kusunoki Yumiko Matsumura Hiroto Izumi Kazuo Asanoma Hiroshi Yagi Ichiro Onoyama Kenzo Sonoda Kimitoshi Kohno Tetsuya Taga Atsuo Itakura Satoru Takeda Kiyoko Kato
author_facet	Izumi Suzuki Sachiko Yoshida Kouichi Tabu Soshi Kusunoki Yumiko Matsumura Hiroto Izumi Kazuo Asanoma Hiroshi Yagi Ichiro Onoyama Kenzo Sonoda Kimitoshi Kohno Tetsuya Taga Atsuo Itakura Satoru Takeda Kiyoko Kato
author_sort	Izumi Suzuki
title	YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
title_short	YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
title_full	YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
title_fullStr	YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
title_full_unstemmed	YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
title_sort	ybx2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2393a0674b334285888ea55f9ddad4b0
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YBX2 and cancer testis antigen 45 contribute to stemness, chemoresistance and a high degree of malignancy in human endometrial cancer

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