Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
<h4>Background</h4>Although it has been estimated that pandemic Influenza A H1N1/2009 has infected millions of people from April to October 2009, a more precise figure requires a worldwide large-scale diagnosis of the presence of Influenza A/H1N1/2009 antibodies within the population. As...
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2010
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oai:doaj.org-article:2395a043cdfb408b8861f05327c904c82021-11-25T06:24:33ZSpecific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.1932-620310.1371/journal.pone.0010176https://doaj.org/article/2395a043cdfb408b8861f05327c904c82010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20418957/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Although it has been estimated that pandemic Influenza A H1N1/2009 has infected millions of people from April to October 2009, a more precise figure requires a worldwide large-scale diagnosis of the presence of Influenza A/H1N1/2009 antibodies within the population. Assays typically used to estimate antibody titers (hemagglutination inhibition and microneutralization) would require the use of the virus, which would seriously limit broad implementation.<h4>Methodology/principal findings</h4>An ELISA method to evaluate the presence and relative concentration of specific Influenza A/H1N1/2009 antibodies in human serum samples is presented. The method is based on the use of a histidine-tagged recombinant fragment of the globular region of the hemagglutinin (HA) of the Influenza A H1N1/2009 virus expressed in E. coli.<h4>Conclusions/significance</h4>The ELISA method consistently discerns between Inf A H1N1 infected and non-infected subjects, particularly after the third week of infection/exposure. Since it does not require the use of viral particles, it can be easily and quickly implemented in any basic laboratory. In addition, in a scenario of insufficient vaccine availability, the use of this ELISA could be useful to determine if a person has some level of specific antibodies against the virus and presumably at least partial protection.Mario M AlvarezFelipe López-PachecoJosé M Aguilar-YañezRoberto Portillo-LaraGonzalo I Mendoza-OchoaSergio García-EchauriPamela FreidenStacey Schultz-CherryManuel I Zertuche-GuerraDavid Bulnes-AbundisJohari Salgado-GallegosLeticia Elizondo-MontemayorMartín Hernández-TorrePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10176 (2010) |
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Medicine R Science Q Mario M Alvarez Felipe López-Pacheco José M Aguilar-Yañez Roberto Portillo-Lara Gonzalo I Mendoza-Ochoa Sergio García-Echauri Pamela Freiden Stacey Schultz-Cherry Manuel I Zertuche-Guerra David Bulnes-Abundis Johari Salgado-Gallegos Leticia Elizondo-Montemayor Martín Hernández-Torre Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| description |
<h4>Background</h4>Although it has been estimated that pandemic Influenza A H1N1/2009 has infected millions of people from April to October 2009, a more precise figure requires a worldwide large-scale diagnosis of the presence of Influenza A/H1N1/2009 antibodies within the population. Assays typically used to estimate antibody titers (hemagglutination inhibition and microneutralization) would require the use of the virus, which would seriously limit broad implementation.<h4>Methodology/principal findings</h4>An ELISA method to evaluate the presence and relative concentration of specific Influenza A/H1N1/2009 antibodies in human serum samples is presented. The method is based on the use of a histidine-tagged recombinant fragment of the globular region of the hemagglutinin (HA) of the Influenza A H1N1/2009 virus expressed in E. coli.<h4>Conclusions/significance</h4>The ELISA method consistently discerns between Inf A H1N1 infected and non-infected subjects, particularly after the third week of infection/exposure. Since it does not require the use of viral particles, it can be easily and quickly implemented in any basic laboratory. In addition, in a scenario of insufficient vaccine availability, the use of this ELISA could be useful to determine if a person has some level of specific antibodies against the virus and presumably at least partial protection. |
| format |
article |
| author |
Mario M Alvarez Felipe López-Pacheco José M Aguilar-Yañez Roberto Portillo-Lara Gonzalo I Mendoza-Ochoa Sergio García-Echauri Pamela Freiden Stacey Schultz-Cherry Manuel I Zertuche-Guerra David Bulnes-Abundis Johari Salgado-Gallegos Leticia Elizondo-Montemayor Martín Hernández-Torre |
| author_facet |
Mario M Alvarez Felipe López-Pacheco José M Aguilar-Yañez Roberto Portillo-Lara Gonzalo I Mendoza-Ochoa Sergio García-Echauri Pamela Freiden Stacey Schultz-Cherry Manuel I Zertuche-Guerra David Bulnes-Abundis Johari Salgado-Gallegos Leticia Elizondo-Montemayor Martín Hernández-Torre |
| author_sort |
Mario M Alvarez |
| title |
Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| title_short |
Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| title_full |
Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| title_fullStr |
Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| title_full_unstemmed |
Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. |
| title_sort |
specific recognition of influenza a/h1n1/2009 antibodies in human serum: a simple virus-free elisa method. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/2395a043cdfb408b8861f05327c904c8 |
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