Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential

Abstract The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is an evolving rese...

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Autores principales:	Refael Minnes, Mati Nissinmann, Yael Maizels, Gabi Gerlitz, Abraham Katzir, Yosef Raichlin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23998c30ce63456e89860fd0a0704171
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spelling	oai:doaj.org-article:23998c30ce63456e89860fd0a07041712021-12-02T16:07:56ZUsing Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential10.1038/s41598-017-04678-62045-2322https://doaj.org/article/23998c30ce63456e89860fd0a07041712017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04678-6https://doaj.org/toc/2045-2322Abstract The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is an evolving research area, whose main aim is to find the spectral differences between diseased and healthy cells. In the present study, we demonstrate that Attenuated Total Reflection Fourier Transform IR (ATR-FTIR) spectroscopy may be used to determine the metastatic potential of cancer cells. Using the ATR-FTIR spectroscopy, we can identify spectral alterations that are a result of hydration or molecular changes. We examined two murine melanoma cells with a common genetic background but a different metastatic level, and similarly, two human melanoma cells. Our findings revealed that higher metastatic potential correlates with membrane hydration level. Measuring the spectral properties of the cells allows us to determine the membrane hydration levels. Thus, ATR-FTIR spectroscopy has the potential to help in cancer metastasis prognosis.Refael MinnesMati NissinmannYael MaizelsGabi GerlitzAbraham KatzirYosef RaichlinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Refael Minnes Mati Nissinmann Yael Maizels Gabi Gerlitz Abraham Katzir Yosef Raichlin Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
description	Abstract The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is an evolving research area, whose main aim is to find the spectral differences between diseased and healthy cells. In the present study, we demonstrate that Attenuated Total Reflection Fourier Transform IR (ATR-FTIR) spectroscopy may be used to determine the metastatic potential of cancer cells. Using the ATR-FTIR spectroscopy, we can identify spectral alterations that are a result of hydration or molecular changes. We examined two murine melanoma cells with a common genetic background but a different metastatic level, and similarly, two human melanoma cells. Our findings revealed that higher metastatic potential correlates with membrane hydration level. Measuring the spectral properties of the cells allows us to determine the membrane hydration levels. Thus, ATR-FTIR spectroscopy has the potential to help in cancer metastasis prognosis.
format	article
author	Refael Minnes Mati Nissinmann Yael Maizels Gabi Gerlitz Abraham Katzir Yosef Raichlin
author_facet	Refael Minnes Mati Nissinmann Yael Maizels Gabi Gerlitz Abraham Katzir Yosef Raichlin
author_sort	Refael Minnes
title	Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
title_short	Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
title_full	Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
title_fullStr	Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
title_full_unstemmed	Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential
title_sort	using attenuated total reflection–fourier transform infra-red (atr-ftir) spectroscopy to distinguish between melanoma cells with a different metastatic potential
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/23998c30ce63456e89860fd0a0704171
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Using Attenuated Total Reflection–Fourier Transform Infra-Red (ATR-FTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential

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