High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).

Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has ena...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Livia Donaire, Laia Pedrola, Raúl de la Rosa, César Llave
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/239a8862758741a59474136f324cc059
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:239a8862758741a59474136f324cc059
record_format	dspace
spelling	oai:doaj.org-article:239a8862758741a59474136f324cc0592021-11-18T07:33:32ZHigh-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).1932-620310.1371/journal.pone.0027916https://doaj.org/article/239a8862758741a59474136f324cc0592011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22140484/?tool=EBIhttps://doaj.org/toc/1932-6203Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA) regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive.Livia DonaireLaia PedrolaRaúl de la RosaCésar LlavePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27916 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Livia Donaire Laia Pedrola Raúl de la Rosa César Llave High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
description	Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA) regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive.
format	article
author	Livia Donaire Laia Pedrola Raúl de la Rosa César Llave
author_facet	Livia Donaire Laia Pedrola Raúl de la Rosa César Llave
author_sort	Livia Donaire
title	High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
title_short	High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
title_full	High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
title_fullStr	High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
title_full_unstemmed	High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
title_sort	high-throughput sequencing of rna silencing-associated small rnas in olive (olea europaea l.).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/239a8862758741a59474136f324cc059
work_keys_str_mv	AT liviadonaire highthroughputsequencingofrnasilencingassociatedsmallrnasinoliveoleaeuropaeal AT laiapedrola highthroughputsequencingofrnasilencingassociatedsmallrnasinoliveoleaeuropaeal AT rauldelarosa highthroughputsequencingofrnasilencingassociatedsmallrnasinoliveoleaeuropaeal AT cesarllave highthroughputsequencingofrnasilencingassociatedsmallrnasinoliveoleaeuropaeal
_version_	1718423272828174336

High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).

Ejemplares similares