The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance

Klebsiella pneumoniae complex (KPC) accounts for approximately one-third of all Gram-negative infections. Moreover, it is highly resistant and can taxonomically be distributed into KpI, KpII, and KpIII phylogroups. This study aimed to investigate the distribution of phylogenetic groups and the relat...

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Autores principales:	Shabnam Baghbanijavid, Hossein Samadi Kafil, Safar Farajniya, Seyed Reza Moaddab, Hasan Hosainzadegan, Fatemeh Yeganeh Sefidan, Mojtaba Varshouchi, Hamed Ebrahimzadeh Leylabadlo, Reza Ghotaslou
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medical emergencies. Critical care. Intensive care. First aid RC86-88.9
Acceso en línea:	https://doaj.org/article/239c0e9fd84842edb9e6030b62cadd3d
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spelling	oai:doaj.org-article:239c0e9fd84842edb9e6030b62cadd3d2021-11-15T01:20:10ZThe Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance2090-285910.1155/2021/1316992https://doaj.org/article/239c0e9fd84842edb9e6030b62cadd3d2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1316992https://doaj.org/toc/2090-2859Klebsiella pneumoniae complex (KPC) accounts for approximately one-third of all Gram-negative infections. Moreover, it is highly resistant and can taxonomically be distributed into KpI, KpII, and KpIII phylogroups. This study aimed to investigate the distribution of phylogenetic groups and the relationship between them and antibiotic resistance patterns. For this purpose, we collected KPC isolates from Tabriz, Iran, between 2018 and 2020. Antimicrobial susceptibility testing was performed by disk diffusion agar, and phylogenetic groups were then examined using gyrA restriction fragment length polymorphism (RFLP) and parC PCR methods. A total of 100 KPC isolates were obtained from the clinical specimens (urine, respiratory secretion, blood, wounds, and trachea). The enrolled patients included 47 men and 53 women aged from 1 to 91 years old. The highest sensitivity was found related to fosfomycin as 85%, followed by amikacin as 66%. The three phylogenetically groups by the RFLP-PCR method were found in KPC, 96% (96 isolates) as KpI, 3% (3 isolates) as KpII, and 1% (1isolate) as KpIII. The highest antibiotic resistance was observed in KpI. It was shown that a valid identification of three phylogenetic groups of KPC can be done by combining both gyrA PCR-RFLP and parC PCR. Of note, the KpI group was also observed as the dominant phylogenetic group with the highest resistance to antibiotics.Shabnam BaghbanijavidHossein Samadi KafilSafar FarajniyaSeyed Reza MoaddabHasan HosainzadeganFatemeh Yeganeh SefidanMojtaba VarshouchiHamed Ebrahimzadeh LeylabadloReza GhotaslouHindawi LimitedarticleMedical emergencies. Critical care. Intensive care. First aidRC86-88.9ENEmergency Medicine International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medical emergencies. Critical care. Intensive care. First aid RC86-88.9
spellingShingle	Medical emergencies. Critical care. Intensive care. First aid RC86-88.9 Shabnam Baghbanijavid Hossein Samadi Kafil Safar Farajniya Seyed Reza Moaddab Hasan Hosainzadegan Fatemeh Yeganeh Sefidan Mojtaba Varshouchi Hamed Ebrahimzadeh Leylabadlo Reza Ghotaslou The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
description	Klebsiella pneumoniae complex (KPC) accounts for approximately one-third of all Gram-negative infections. Moreover, it is highly resistant and can taxonomically be distributed into KpI, KpII, and KpIII phylogroups. This study aimed to investigate the distribution of phylogenetic groups and the relationship between them and antibiotic resistance patterns. For this purpose, we collected KPC isolates from Tabriz, Iran, between 2018 and 2020. Antimicrobial susceptibility testing was performed by disk diffusion agar, and phylogenetic groups were then examined using gyrA restriction fragment length polymorphism (RFLP) and parC PCR methods. A total of 100 KPC isolates were obtained from the clinical specimens (urine, respiratory secretion, blood, wounds, and trachea). The enrolled patients included 47 men and 53 women aged from 1 to 91 years old. The highest sensitivity was found related to fosfomycin as 85%, followed by amikacin as 66%. The three phylogenetically groups by the RFLP-PCR method were found in KPC, 96% (96 isolates) as KpI, 3% (3 isolates) as KpII, and 1% (1isolate) as KpIII. The highest antibiotic resistance was observed in KpI. It was shown that a valid identification of three phylogenetic groups of KPC can be done by combining both gyrA PCR-RFLP and parC PCR. Of note, the KpI group was also observed as the dominant phylogenetic group with the highest resistance to antibiotics.
format	article
author	Shabnam Baghbanijavid Hossein Samadi Kafil Safar Farajniya Seyed Reza Moaddab Hasan Hosainzadegan Fatemeh Yeganeh Sefidan Mojtaba Varshouchi Hamed Ebrahimzadeh Leylabadlo Reza Ghotaslou
author_facet	Shabnam Baghbanijavid Hossein Samadi Kafil Safar Farajniya Seyed Reza Moaddab Hasan Hosainzadegan Fatemeh Yeganeh Sefidan Mojtaba Varshouchi Hamed Ebrahimzadeh Leylabadlo Reza Ghotaslou
author_sort	Shabnam Baghbanijavid
title	The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
title_short	The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
title_full	The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
title_fullStr	The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
title_full_unstemmed	The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance
title_sort	association of the phylogenetic typing of the klebsiella pneumoniae isolates with antibiotic resistance
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/239c0e9fd84842edb9e6030b62cadd3d
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The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance

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