Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.

We evaluated phytochemical composition, antibacterial, antifungal, anti-oxidant and cytotoxic properties of aqueous (water) and organic extracts (methanol, ethyl acetate and n-hexane) of Chenopodium glaucum. Highest phenolic content 45 mg gallic acid equivalents (GAE)/g d.w was found in aqueous extr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Safi Ullah Khan, Faizan Ullah, Sultan Mehmood, Shah Fahad, Arsalan Ahmad Rahi, Fayez Althobaiti, Eldessoky S. Dessoky, Shah Saud, Subhan Danish, Rahul Datta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/239d3af1553a46bb9e01cbb17f14b715
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:239d3af1553a46bb9e01cbb17f14b715
record_format	dspace
spelling	oai:doaj.org-article:239d3af1553a46bb9e01cbb17f14b7152021-11-04T07:42:08ZAntimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.1932-6203https://doaj.org/article/239d3af1553a46bb9e01cbb17f14b7152021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555819/?tool=EBIhttps://doaj.org/toc/1932-6203We evaluated phytochemical composition, antibacterial, antifungal, anti-oxidant and cytotoxic properties of aqueous (water) and organic extracts (methanol, ethyl acetate and n-hexane) of Chenopodium glaucum. Highest phenolic content 45 mg gallic acid equivalents (GAE)/g d.w was found in aqueous extract followed by ethyl acetate (41mg GAE/g d.w) and methanol extract (34.46 mg GAE/g d.w). Antibacterial potential of aqueous and organic extracts of C. glaucum was examined against Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli and Staphylococcus epidermidis. The aqueous, methanolic, ethyl acetate, and n-hexane extract showed antibacterial activity against A. baumannii, K. pneumoniae, E. coli and S. epidermidis. However, against A. baumannii significantly higher inhibition zone (19 mm and 18.96 mm respectively) was shown by ethyl acetate and methanol extracts. Aqueous extract possessed highest growth inhibition (11 mm) against E. coli. Aqueous, ethyl acetate and methanol extracts showed 9 mm, 10 mm, and 10.33 mm zone of inhibition against the K. pneumoniae. For antifungal activity, the extracts were less effective against Aspergillus niger but showed strong antifungal activity against Aspergillus flavus (A. flavus). The antioxidant activity was measured as DPPH (2, 2-diphenyl-1-picrylhydrazyl), H2O2 and ABTS (2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity of free radicals. All the organic extracts of C. glaucum possessed ABTS, DPPH and H2O2 scavenging properties. The highest cytotoxic activity measured as half maximal inhibitory concentration (IC50) against human lungs carcinoma cells was recorded for methanolic (IC50 = 16 μg/mL) and n-hexane (IC50 = 25 μg/mL) extracts, respectively. The Gas chromatography–mass spectrometry (GC-MS) analysis showed 4 major and 26 minor compounds in n-hexane extract and 4 major and 7 minor compounds in methanol extract of the C. glaucum. It is concluded that aqueous and organic extracts of C. glaucum would be potential therapeutic agents and could be exploited on a pilot scale to treat human pathogenic diseases.Safi Ullah KhanFaizan UllahSultan MehmoodShah FahadArsalan Ahmad RahiFayez AlthobaitiEldessoky S. DessokyShah SaudSubhan DanishRahul DattaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Safi Ullah Khan Faizan Ullah Sultan Mehmood Shah Fahad Arsalan Ahmad Rahi Fayez Althobaiti Eldessoky S. Dessoky Shah Saud Subhan Danish Rahul Datta Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
description	We evaluated phytochemical composition, antibacterial, antifungal, anti-oxidant and cytotoxic properties of aqueous (water) and organic extracts (methanol, ethyl acetate and n-hexane) of Chenopodium glaucum. Highest phenolic content 45 mg gallic acid equivalents (GAE)/g d.w was found in aqueous extract followed by ethyl acetate (41mg GAE/g d.w) and methanol extract (34.46 mg GAE/g d.w). Antibacterial potential of aqueous and organic extracts of C. glaucum was examined against Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli and Staphylococcus epidermidis. The aqueous, methanolic, ethyl acetate, and n-hexane extract showed antibacterial activity against A. baumannii, K. pneumoniae, E. coli and S. epidermidis. However, against A. baumannii significantly higher inhibition zone (19 mm and 18.96 mm respectively) was shown by ethyl acetate and methanol extracts. Aqueous extract possessed highest growth inhibition (11 mm) against E. coli. Aqueous, ethyl acetate and methanol extracts showed 9 mm, 10 mm, and 10.33 mm zone of inhibition against the K. pneumoniae. For antifungal activity, the extracts were less effective against Aspergillus niger but showed strong antifungal activity against Aspergillus flavus (A. flavus). The antioxidant activity was measured as DPPH (2, 2-diphenyl-1-picrylhydrazyl), H2O2 and ABTS (2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity of free radicals. All the organic extracts of C. glaucum possessed ABTS, DPPH and H2O2 scavenging properties. The highest cytotoxic activity measured as half maximal inhibitory concentration (IC50) against human lungs carcinoma cells was recorded for methanolic (IC50 = 16 μg/mL) and n-hexane (IC50 = 25 μg/mL) extracts, respectively. The Gas chromatography–mass spectrometry (GC-MS) analysis showed 4 major and 26 minor compounds in n-hexane extract and 4 major and 7 minor compounds in methanol extract of the C. glaucum. It is concluded that aqueous and organic extracts of C. glaucum would be potential therapeutic agents and could be exploited on a pilot scale to treat human pathogenic diseases.
format	article
author	Safi Ullah Khan Faizan Ullah Sultan Mehmood Shah Fahad Arsalan Ahmad Rahi Fayez Althobaiti Eldessoky S. Dessoky Shah Saud Subhan Danish Rahul Datta
author_facet	Safi Ullah Khan Faizan Ullah Sultan Mehmood Shah Fahad Arsalan Ahmad Rahi Fayez Althobaiti Eldessoky S. Dessoky Shah Saud Subhan Danish Rahul Datta
author_sort	Safi Ullah Khan
title	Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
title_short	Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
title_full	Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
title_fullStr	Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
title_full_unstemmed	Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.
title_sort	antimicrobial, antioxidant and cytotoxic properties of chenopodium glaucum l.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/239d3af1553a46bb9e01cbb17f14b715
work_keys_str_mv	AT safiullahkhan antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT faizanullah antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT sultanmehmood antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT shahfahad antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT arsalanahmadrahi antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT fayezalthobaiti antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT eldessokysdessoky antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT shahsaud antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT subhandanish antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml AT rahuldatta antimicrobialantioxidantandcytotoxicpropertiesofchenopodiumglaucuml
_version_	1718445007109619712

Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.

Ejemplares similares