Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro

Abstract Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine li...

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Autores principales:	Julia Fernández-Pérez, Marcus Binner, Carsten Werner, Laura J. Bray
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/239f4dfe0a304a129a39ad20176ab67b
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spelling	oai:doaj.org-article:239f4dfe0a304a129a39ad20176ab67b2021-12-02T16:06:47ZLimbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro10.1038/s41598-017-06898-22045-2322https://doaj.org/article/239f4dfe0a304a129a39ad20176ab67b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06898-2https://doaj.org/toc/2045-2322Abstract Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine limbal epithelial stem cells (pLESCs). The isolated cells adhered to plastic and grew in monolayers in vitro using serum-supplemented or serum-free medium. The pLSCs demonstrated expression of CD29, and cross-reactivity with anti-human CD45, CD90, CD105, CD146, and HLA-ABC. However, expression of CD105, CD146 and HLA-ABC reduced when cultured in serum-free medium. PLSCs did not undergo adipogenic or osteogenic differentiation, but differentiated towards the chondrogenic lineage. Isolated cells were also co-cultured with human umbilical vein endothelial cells (HUVECs) in star-shaped Poly(ethylene glycol) (starPEG)-heparin hydrogels to assess their pericyte capacity which supported angiogenesis networks of HUVECs. PLSCs supported the three dimensional HUVEC network for 7 days. The isolated cells were further growth-arrested and evaluated as feeder cells for pLESC expansion on silk fibroin membranes, as a potential carrier material for transplantation. PLSCs supported the growth of pLESCs comparably to murine 3T3 cells. In conclusion, although pLSCs were not completely comparable to their human counterpart, they display several mesenchymal-like characteristics in vitro.Julia Fernández-PérezMarcus BinnerCarsten WernerLaura J. BrayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Fernández-Pérez Marcus Binner Carsten Werner Laura J. Bray Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
description	Abstract Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine limbal epithelial stem cells (pLESCs). The isolated cells adhered to plastic and grew in monolayers in vitro using serum-supplemented or serum-free medium. The pLSCs demonstrated expression of CD29, and cross-reactivity with anti-human CD45, CD90, CD105, CD146, and HLA-ABC. However, expression of CD105, CD146 and HLA-ABC reduced when cultured in serum-free medium. PLSCs did not undergo adipogenic or osteogenic differentiation, but differentiated towards the chondrogenic lineage. Isolated cells were also co-cultured with human umbilical vein endothelial cells (HUVECs) in star-shaped Poly(ethylene glycol) (starPEG)-heparin hydrogels to assess their pericyte capacity which supported angiogenesis networks of HUVECs. PLSCs supported the three dimensional HUVEC network for 7 days. The isolated cells were further growth-arrested and evaluated as feeder cells for pLESC expansion on silk fibroin membranes, as a potential carrier material for transplantation. PLSCs supported the growth of pLESCs comparably to murine 3T3 cells. In conclusion, although pLSCs were not completely comparable to their human counterpart, they display several mesenchymal-like characteristics in vitro.
format	article
author	Julia Fernández-Pérez Marcus Binner Carsten Werner Laura J. Bray
author_facet	Julia Fernández-Pérez Marcus Binner Carsten Werner Laura J. Bray
author_sort	Julia Fernández-Pérez
title	Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
title_short	Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
title_full	Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
title_fullStr	Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
title_full_unstemmed	Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
title_sort	limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/239f4dfe0a304a129a39ad20176ab67b
work_keys_str_mv	AT juliafernandezperez limbalstromalcellsderivedfromporcinetissuedemonstratemesenchymalcharacteristicsinvitro AT marcusbinner limbalstromalcellsderivedfromporcinetissuedemonstratemesenchymalcharacteristicsinvitro AT carstenwerner limbalstromalcellsderivedfromporcinetissuedemonstratemesenchymalcharacteristicsinvitro AT laurajbray limbalstromalcellsderivedfromporcinetissuedemonstratemesenchymalcharacteristicsinvitro
_version_	1718384829701029888

Limbal stromal cells derived from porcine tissue demonstrate mesenchymal characteristics in vitro

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