Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease.
Nicastrin is an obligatory component of the γ-secretase; the enzyme complex that leads to the production of Aβ fragments critically central to the pathogenesis of Alzheimer's disease (AD). Analyses of the effects of common variation in this gene on risk for late onset AD have been inconclusive....
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2011
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oai:doaj.org-article:23a00884875846cc8154fa1c9d1e808c2021-11-18T06:58:13ZDeep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease.1932-620310.1371/journal.pone.0017298https://doaj.org/article/23a00884875846cc8154fa1c9d1e808c2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21364883/?tool=EBIhttps://doaj.org/toc/1932-6203Nicastrin is an obligatory component of the γ-secretase; the enzyme complex that leads to the production of Aβ fragments critically central to the pathogenesis of Alzheimer's disease (AD). Analyses of the effects of common variation in this gene on risk for late onset AD have been inconclusive. We investigated the effect of rare variation in the coding regions of the Nicastrin gene in a cohort of AD patients and matched controls using an innovative pooling approach and next generation sequencing. Five SNPs were identified and validated by individual genotyping from 311 cases and 360 controls. Association analysis identified a non-synonymous rare SNP (N417Y) with a statistically higher frequency in cases compared to controls in the Greek population (OR 3.994, CI 1.105-14.439, p = 0.035). This finding warrants further investigation in a larger cohort and adds weight to the hypothesis that rare variation explains some of genetic heritability still to be identified in Alzheimer's disease.Michelle K LuptonPetroula ProitsiMakrina DanillidouMagda TsolakiGillian HamiltonRichard WroeMegan PritchardKathryn LordBelinda M MartinIwona KloszewskaHilkka SoininenPatrizia MecocciBruno VellasDenise HaroldPaul HollingworthSimon LovestoneJohn F PowellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17298 (2011) |
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Medicine R Science Q |
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Medicine R Science Q Michelle K Lupton Petroula Proitsi Makrina Danillidou Magda Tsolaki Gillian Hamilton Richard Wroe Megan Pritchard Kathryn Lord Belinda M Martin Iwona Kloszewska Hilkka Soininen Patrizia Mecocci Bruno Vellas Denise Harold Paul Hollingworth Simon Lovestone John F Powell Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| description |
Nicastrin is an obligatory component of the γ-secretase; the enzyme complex that leads to the production of Aβ fragments critically central to the pathogenesis of Alzheimer's disease (AD). Analyses of the effects of common variation in this gene on risk for late onset AD have been inconclusive. We investigated the effect of rare variation in the coding regions of the Nicastrin gene in a cohort of AD patients and matched controls using an innovative pooling approach and next generation sequencing. Five SNPs were identified and validated by individual genotyping from 311 cases and 360 controls. Association analysis identified a non-synonymous rare SNP (N417Y) with a statistically higher frequency in cases compared to controls in the Greek population (OR 3.994, CI 1.105-14.439, p = 0.035). This finding warrants further investigation in a larger cohort and adds weight to the hypothesis that rare variation explains some of genetic heritability still to be identified in Alzheimer's disease. |
| format |
article |
| author |
Michelle K Lupton Petroula Proitsi Makrina Danillidou Magda Tsolaki Gillian Hamilton Richard Wroe Megan Pritchard Kathryn Lord Belinda M Martin Iwona Kloszewska Hilkka Soininen Patrizia Mecocci Bruno Vellas Denise Harold Paul Hollingworth Simon Lovestone John F Powell |
| author_facet |
Michelle K Lupton Petroula Proitsi Makrina Danillidou Magda Tsolaki Gillian Hamilton Richard Wroe Megan Pritchard Kathryn Lord Belinda M Martin Iwona Kloszewska Hilkka Soininen Patrizia Mecocci Bruno Vellas Denise Harold Paul Hollingworth Simon Lovestone John F Powell |
| author_sort |
Michelle K Lupton |
| title |
Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| title_short |
Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| title_full |
Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| title_fullStr |
Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| title_full_unstemmed |
Deep sequencing of the Nicastrin gene in pooled DNA, the identification of genetic variants that affect risk of Alzheimer's disease. |
| title_sort |
deep sequencing of the nicastrin gene in pooled dna, the identification of genetic variants that affect risk of alzheimer's disease. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/23a00884875846cc8154fa1c9d1e808c |
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