Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR

ABSTRACT The common environmental bacterium and opportunistic pathogen Pseudomonas aeruginosa encodes diverse metabolic pathways and associated regulatory networks allowing it to thrive in these different environments. In an effort to understand P. aeruginosa metabolism and detection of host-derived...

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Autores principales:	Jamie A. Meadows, Matthew J. Wargo
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	metabolism osmoprotectant quaternary amine transcriptional regulation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/23a29628126c43a19e49dc8308ea2ff9
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spelling	oai:doaj.org-article:23a29628126c43a19e49dc8308ea2ff92021-11-15T15:22:01ZTranscriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR10.1128/mSphere.00480-172379-5042https://doaj.org/article/23a29628126c43a19e49dc8308ea2ff92018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00480-17https://doaj.org/toc/2379-5042ABSTRACT The common environmental bacterium and opportunistic pathogen Pseudomonas aeruginosa encodes diverse metabolic pathways and associated regulatory networks allowing it to thrive in these different environments. In an effort to understand P. aeruginosa metabolism and detection of host-derived compounds, we previously identified CdhR and GbdR as members of the AraC transcription factor family that regulate catabolism of the quaternary amine compounds carnitine and glycine betaine, respectively. In this study, our goal was to further characterize regulation of carnitine catabolism by the transcription factor CdhR. CdhR binds in a concentration-dependent manner upstream of the carnitine catabolism operon promoter (PcaiXcdhCABhocS). We identified the CdhR binding site and determined that it overlaps with the GbdR binding site in the caiX-cdhR intergenic region. Carnitine catabolism is repressed by glucose and glycine betaine, and here we show this happens at the transcriptional level. Furthermore, we show that CdhR enhances its own expression and that GbdR contributes to cdhR expression by enhancing the level of basal expression. The intertwined regulation of caiX and cdhR transcription by GbdR and CdhR suggests that carnitine catabolism is under tight but tuneable control. IMPORTANCE Pathogens must metabolize host-derived compounds during infection and properly regulate the responsible pathways. Carnitine is a common eukaryotic-associated quaternary amine compound that can be catabolized by Pseudomonas aeruginosa. Here we expand on our understanding of how this metabolic pathway is regulated and provide details on how carnitine catabolism is intertwined with glycine betaine catabolism at the level of transcriptional control.Jamie A. MeadowsMatthew J. WargoAmerican Society for Microbiologyarticlemetabolismosmoprotectantquaternary aminetranscriptional regulationMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	metabolism osmoprotectant quaternary amine transcriptional regulation Microbiology QR1-502
spellingShingle	metabolism osmoprotectant quaternary amine transcriptional regulation Microbiology QR1-502 Jamie A. Meadows Matthew J. Wargo Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
description	ABSTRACT The common environmental bacterium and opportunistic pathogen Pseudomonas aeruginosa encodes diverse metabolic pathways and associated regulatory networks allowing it to thrive in these different environments. In an effort to understand P. aeruginosa metabolism and detection of host-derived compounds, we previously identified CdhR and GbdR as members of the AraC transcription factor family that regulate catabolism of the quaternary amine compounds carnitine and glycine betaine, respectively. In this study, our goal was to further characterize regulation of carnitine catabolism by the transcription factor CdhR. CdhR binds in a concentration-dependent manner upstream of the carnitine catabolism operon promoter (PcaiXcdhCABhocS). We identified the CdhR binding site and determined that it overlaps with the GbdR binding site in the caiX-cdhR intergenic region. Carnitine catabolism is repressed by glucose and glycine betaine, and here we show this happens at the transcriptional level. Furthermore, we show that CdhR enhances its own expression and that GbdR contributes to cdhR expression by enhancing the level of basal expression. The intertwined regulation of caiX and cdhR transcription by GbdR and CdhR suggests that carnitine catabolism is under tight but tuneable control. IMPORTANCE Pathogens must metabolize host-derived compounds during infection and properly regulate the responsible pathways. Carnitine is a common eukaryotic-associated quaternary amine compound that can be catabolized by Pseudomonas aeruginosa. Here we expand on our understanding of how this metabolic pathway is regulated and provide details on how carnitine catabolism is intertwined with glycine betaine catabolism at the level of transcriptional control.
format	article
author	Jamie A. Meadows Matthew J. Wargo
author_facet	Jamie A. Meadows Matthew J. Wargo
author_sort	Jamie A. Meadows
title	Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
title_short	Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
title_full	Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
title_fullStr	Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
title_full_unstemmed	Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR
title_sort	transcriptional regulation of carnitine catabolism in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content> by cdhr
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/23a29628126c43a19e49dc8308ea2ff9
work_keys_str_mv	AT jamieameadows transcriptionalregulationofcarnitinecatabolisminnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontentbycdhr AT matthewjwargo transcriptionalregulationofcarnitinecatabolisminnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontentbycdhr
_version_	1718428058151550976

Transcriptional Regulation of Carnitine Catabolism in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> by CdhR

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