Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy

Abstract The current lack of a reliable biomarker of disease activity in anti-neutrophil cytoplasmic autoantibody (ANCA) associated vasculitis poses a significant clinical unmet need when determining relapsing or persisting disease. In this study, we demonstrate for the first time that attenuated to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adam D. Morris, Camilo L. M. Morais, Kássio M. G. Lima, Daniel L. D. Freitas, Mark E. Brady, Ajay P. Dhaygude, Anthony W. Rowbottom, Francis L. Martin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23acb19535a346939d22f09332fcf8e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:23acb19535a346939d22f09332fcf8e9
record_format	dspace
spelling	oai:doaj.org-article:23acb19535a346939d22f09332fcf8e92021-12-02T14:35:40ZDistinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy10.1038/s41598-021-89344-82045-2322https://doaj.org/article/23acb19535a346939d22f09332fcf8e92021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89344-8https://doaj.org/toc/2045-2322Abstract The current lack of a reliable biomarker of disease activity in anti-neutrophil cytoplasmic autoantibody (ANCA) associated vasculitis poses a significant clinical unmet need when determining relapsing or persisting disease. In this study, we demonstrate for the first time that attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy offers a novel and functional candidate biomarker, distinguishing active from quiescent disease with a high degree of accuracy. Paired blood and urine samples were collected within a single UK centre from patients with active disease, disease remission, disease controls and healthy controls. Three key biofluids were evaluated; plasma, serum and urine, with subsequent chemometric analysis and blind predictive model validation. Spectrochemical interrogation proved plasma to be the most conducive biofluid, with excellent separation between the two categories on PC2 direction (AUC 0.901) and 100% sensitivity (F-score 92.3%) for disease remission and 85.7% specificity (F-score 92.3%) for active disease on blind predictive modelling. This was independent of organ system involvement and current ANCA status, with similar findings observed on comparative analysis following successful remission-induction therapy (AUC > 0.9, 100% sensitivity for disease remission, F-score 75%). This promising technique is clinically translatable and warrants future larger study with longitudinal data, potentially aiding earlier intervention and individualisation of treatment.Adam D. MorrisCamilo L. M. MoraisKássio M. G. LimaDaniel L. D. FreitasMark E. BradyAjay P. DhaygudeAnthony W. RowbottomFrancis L. MartinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adam D. Morris Camilo L. M. Morais Kássio M. G. Lima Daniel L. D. Freitas Mark E. Brady Ajay P. Dhaygude Anthony W. Rowbottom Francis L. Martin Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
description	Abstract The current lack of a reliable biomarker of disease activity in anti-neutrophil cytoplasmic autoantibody (ANCA) associated vasculitis poses a significant clinical unmet need when determining relapsing or persisting disease. In this study, we demonstrate for the first time that attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy offers a novel and functional candidate biomarker, distinguishing active from quiescent disease with a high degree of accuracy. Paired blood and urine samples were collected within a single UK centre from patients with active disease, disease remission, disease controls and healthy controls. Three key biofluids were evaluated; plasma, serum and urine, with subsequent chemometric analysis and blind predictive model validation. Spectrochemical interrogation proved plasma to be the most conducive biofluid, with excellent separation between the two categories on PC2 direction (AUC 0.901) and 100% sensitivity (F-score 92.3%) for disease remission and 85.7% specificity (F-score 92.3%) for active disease on blind predictive modelling. This was independent of organ system involvement and current ANCA status, with similar findings observed on comparative analysis following successful remission-induction therapy (AUC > 0.9, 100% sensitivity for disease remission, F-score 75%). This promising technique is clinically translatable and warrants future larger study with longitudinal data, potentially aiding earlier intervention and individualisation of treatment.
format	article
author	Adam D. Morris Camilo L. M. Morais Kássio M. G. Lima Daniel L. D. Freitas Mark E. Brady Ajay P. Dhaygude Anthony W. Rowbottom Francis L. Martin
author_facet	Adam D. Morris Camilo L. M. Morais Kássio M. G. Lima Daniel L. D. Freitas Mark E. Brady Ajay P. Dhaygude Anthony W. Rowbottom Francis L. Martin
author_sort	Adam D. Morris
title	Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
title_short	Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
title_full	Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
title_fullStr	Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
title_full_unstemmed	Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy
title_sort	distinguishing active from quiescent disease in anca-associated vasculitis using attenuated total reflection fourier-transform infrared spectroscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/23acb19535a346939d22f09332fcf8e9
work_keys_str_mv	AT adamdmorris distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT camilolmmorais distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT kassiomglima distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT danielldfreitas distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT markebrady distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT ajaypdhaygude distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT anthonywrowbottom distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy AT francislmartin distinguishingactivefromquiescentdiseaseinancaassociatedvasculitisusingattenuatedtotalreflectionfouriertransforminfraredspectroscopy
_version_	1718391059034144768

Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy

Ejemplares similares