Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel

Abstract Although atelocollagen gel is used as a scaffold for culturing human articular cartilage-derived chondrocytes, little is known about cell–gel interactions. In this study, we investigated the mechanism via which atelocollagen gel affects human articular cartilage-derived chondrocytes. Two ty...

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Autores principales:	Takashi Kanamoto, Minami Hikida, Seira Sato, Shohei Oyama, Yoshihito Tachi, Sanae Kuroda, Takeo Mazuka, Kosuke Ebina, Tsuyoshi Nakai, Ken Nakata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23b003eaece04ed3b4bbc9ffdccf6576
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spelling	oai:doaj.org-article:23b003eaece04ed3b4bbc9ffdccf65762021-12-02T15:23:39ZIntegrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel10.1038/s41598-021-81378-22045-2322https://doaj.org/article/23b003eaece04ed3b4bbc9ffdccf65762021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81378-2https://doaj.org/toc/2045-2322Abstract Although atelocollagen gel is used as a scaffold for culturing human articular cartilage-derived chondrocytes, little is known about cell–gel interactions. In this study, we investigated the mechanism via which atelocollagen gel affects human articular cartilage-derived chondrocytes. Two types of three-dimensional cultures of human articular cartilage-derived chondrocytes (i.e., with and without atelocollagen gel) were compared. While the amount of atelocollagen gel in culture gradually decreased with time, it promoted the expression of matrix metalloproteinases (MMPs) during the early stages of culture. Genome-wide differential gene expression analysis revealed that cell membrane- and extracellular matrix-related genes were highly ranked among up- and down-regulated groups in cells cultured in the presence of atelocollagen gel. Among the integrin family of genes, the expression of integrin subunit alpha 2 and integrin subunit alpha 10 was significantly increased in the presence of atelocollagen gel. Blocking α2β1 integrin with the specific inhibitor BTT 3033 had a significant effect on cell proliferation, MMP expression, and cell shape, as well as on the response to mechanical stimulation. Taken together, our findings indicate that the α2β1 integrin pathway plays an important role in the interaction of atelocollagen gel with human articular cartilage-derived chondrocytes and may be a potential therapeutic target for articular cartilage disorders.Takashi KanamotoMinami HikidaSeira SatoShohei OyamaYoshihito TachiSanae KurodaTakeo MazukaKosuke EbinaTsuyoshi NakaiKen NakataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takashi Kanamoto Minami Hikida Seira Sato Shohei Oyama Yoshihito Tachi Sanae Kuroda Takeo Mazuka Kosuke Ebina Tsuyoshi Nakai Ken Nakata Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
description	Abstract Although atelocollagen gel is used as a scaffold for culturing human articular cartilage-derived chondrocytes, little is known about cell–gel interactions. In this study, we investigated the mechanism via which atelocollagen gel affects human articular cartilage-derived chondrocytes. Two types of three-dimensional cultures of human articular cartilage-derived chondrocytes (i.e., with and without atelocollagen gel) were compared. While the amount of atelocollagen gel in culture gradually decreased with time, it promoted the expression of matrix metalloproteinases (MMPs) during the early stages of culture. Genome-wide differential gene expression analysis revealed that cell membrane- and extracellular matrix-related genes were highly ranked among up- and down-regulated groups in cells cultured in the presence of atelocollagen gel. Among the integrin family of genes, the expression of integrin subunit alpha 2 and integrin subunit alpha 10 was significantly increased in the presence of atelocollagen gel. Blocking α2β1 integrin with the specific inhibitor BTT 3033 had a significant effect on cell proliferation, MMP expression, and cell shape, as well as on the response to mechanical stimulation. Taken together, our findings indicate that the α2β1 integrin pathway plays an important role in the interaction of atelocollagen gel with human articular cartilage-derived chondrocytes and may be a potential therapeutic target for articular cartilage disorders.
format	article
author	Takashi Kanamoto Minami Hikida Seira Sato Shohei Oyama Yoshihito Tachi Sanae Kuroda Takeo Mazuka Kosuke Ebina Tsuyoshi Nakai Ken Nakata
author_facet	Takashi Kanamoto Minami Hikida Seira Sato Shohei Oyama Yoshihito Tachi Sanae Kuroda Takeo Mazuka Kosuke Ebina Tsuyoshi Nakai Ken Nakata
author_sort	Takashi Kanamoto
title	Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
title_short	Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
title_full	Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
title_fullStr	Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
title_full_unstemmed	Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
title_sort	integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/23b003eaece04ed3b4bbc9ffdccf6576
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Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel

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