The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice 

Fahimeh Hajiahmadi,1 Mohammad Yousef Alikhani,1,2 Hanifeh Shariatifar,3 Mohammad Reza Arabestani,1,2 Davoud Ahmadvand4,51Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran; 2Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamad...

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Autores principales:	Hajiahmadi F, Alikhani MY, Shariatifar H, Arabestani MR, Ahmadvand D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	MRSA antibacterial activity encapsulation efficiency conjugation efficiency lysostaphin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/23b64d78479340689ee709df78748f0f
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spelling	oai:doaj.org-article:23b64d78479340689ee709df78748f0f2021-12-02T03:14:48ZThe bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice 1178-2013https://doaj.org/article/23b64d78479340689ee709df78748f0f2019-07-01T00:00:00Zhttps://www.dovepress.com/the-bactericidal-effect-of-lysostaphin-coupled-with-liposomal-vancomyc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fahimeh Hajiahmadi,1 Mohammad Yousef Alikhani,1,2 Hanifeh Shariatifar,3 Mohammad Reza Arabestani,1,2 Davoud Ahmadvand4,51Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran; 2Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; 3Young Researches and Elite Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; 4Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; 5Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, IranBackground and aim: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of surgical infection, and its resistance to numerous conventional antibiotics makes treatment difﬁcult. Although vancomycin is often an effective agent for the initial therapy of MRSA, clinical failure sometimes occurs. Therefore, there is an urgent need to develop better therapies. Here, we prepared some vancomycin-loaded nanoliposomes coupled with anti-staphylococcal protein (lysostaphin) and evaluated their in vitro and in vivo efficacy as a topical MRSA therapy.Methods: Vancomycin was encapsulated in liposomes, and the coupling of lysostaphin with the surface of liposomes was carried out through cyanuric functional groups. The bactericidal efficacies and a full characterization were evaluated. To define different nanoliposomal–bacterium interactions and their bactericidal effect, flow cytometry was employed. Finally, in vivo, the topical antibacterial activity of each formulation was measured against surgical wound MRSA infection in a mouse model.Results: High encapsulation and conjugation efficiency were achieved for all formulations. All the formulations showed a significant reduction in bacterial counts (p<0.05). The targeted liposomes more effectively suppress bacterial infection in vitro and in vivo relative to equivalent doses of untargeted vancomycin liposome. The flow cytometry results confirmed liposome–bacterium interactions, which increased during the incubation time. The maximum binding rate and the bactericidal effect were significantly higher in targeted liposomes (p<0.05) compared with control liposomes.Conclusion: Our data suggest a novel nano-vehicle (lysostaphin-conjugated coupled liposomal vancomycin) which could be used as a great topical antimicrobial construct for treatment of MRSA skin infections.Keywords: MRSA, antibacterial activity, encapsulation efficiency, conjugation efficiency, lysostaphinHajiahmadi FAlikhani MYShariatifar HArabestani MRAhmadvand DDove Medical PressarticleMRSAantibacterial activityencapsulation efficiencyconjugation efficiencylysostaphinMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5943-5955 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	MRSA antibacterial activity encapsulation efficiency conjugation efficiency lysostaphin Medicine (General) R5-920
spellingShingle	MRSA antibacterial activity encapsulation efficiency conjugation efficiency lysostaphin Medicine (General) R5-920 Hajiahmadi F Alikhani MY Shariatifar H Arabestani MR Ahmadvand D The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
description	Fahimeh Hajiahmadi,1 Mohammad Yousef Alikhani,1,2 Hanifeh Shariatifar,3 Mohammad Reza Arabestani,1,2 Davoud Ahmadvand4,51Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran; 2Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; 3Young Researches and Elite Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; 4Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; 5Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, IranBackground and aim: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of surgical infection, and its resistance to numerous conventional antibiotics makes treatment difﬁcult. Although vancomycin is often an effective agent for the initial therapy of MRSA, clinical failure sometimes occurs. Therefore, there is an urgent need to develop better therapies. Here, we prepared some vancomycin-loaded nanoliposomes coupled with anti-staphylococcal protein (lysostaphin) and evaluated their in vitro and in vivo efficacy as a topical MRSA therapy.Methods: Vancomycin was encapsulated in liposomes, and the coupling of lysostaphin with the surface of liposomes was carried out through cyanuric functional groups. The bactericidal efficacies and a full characterization were evaluated. To define different nanoliposomal–bacterium interactions and their bactericidal effect, flow cytometry was employed. Finally, in vivo, the topical antibacterial activity of each formulation was measured against surgical wound MRSA infection in a mouse model.Results: High encapsulation and conjugation efficiency were achieved for all formulations. All the formulations showed a significant reduction in bacterial counts (p<0.05). The targeted liposomes more effectively suppress bacterial infection in vitro and in vivo relative to equivalent doses of untargeted vancomycin liposome. The flow cytometry results confirmed liposome–bacterium interactions, which increased during the incubation time. The maximum binding rate and the bactericidal effect were significantly higher in targeted liposomes (p<0.05) compared with control liposomes.Conclusion: Our data suggest a novel nano-vehicle (lysostaphin-conjugated coupled liposomal vancomycin) which could be used as a great topical antimicrobial construct for treatment of MRSA skin infections.Keywords: MRSA, antibacterial activity, encapsulation efficiency, conjugation efficiency, lysostaphin
format	article
author	Hajiahmadi F Alikhani MY Shariatifar H Arabestani MR Ahmadvand D
author_facet	Hajiahmadi F Alikhani MY Shariatifar H Arabestani MR Ahmadvand D
author_sort	Hajiahmadi F
title	The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
title_short	The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
title_full	The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
title_fullStr	The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
title_full_unstemmed	The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice
title_sort	bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant staphylococcus aureus infected skin wounds in mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/23b64d78479340689ee709df78748f0f
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The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice&nbsp;

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The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice