Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma

Abstract Increasing evidence demonstrates an important role for the extracellular matrix (ECM) in breast cancer progression. Collagen type I, a core constituent of the fibrous ECM, undergoes a significant set of changes that accompany tumor progression, termed Tumor Associated Collagen Signatures (T...

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Autores principales:	Lucas A. Tomko, Ryan C. Hill, Alexander Barrett, Joseph M. Szulczewski, Matthew W. Conklin, Kevin W. Eliceiri, Patricia J. Keely, Kirk C. Hansen, Suzanne M. Ponik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23bb514e9fc84cb880e52835f19ae0f8
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spelling	oai:doaj.org-article:23bb514e9fc84cb880e52835f19ae0f82021-12-02T15:08:23ZTargeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma10.1038/s41598-018-31126-w2045-2322https://doaj.org/article/23bb514e9fc84cb880e52835f19ae0f82018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31126-whttps://doaj.org/toc/2045-2322Abstract Increasing evidence demonstrates an important role for the extracellular matrix (ECM) in breast cancer progression. Collagen type I, a core constituent of the fibrous ECM, undergoes a significant set of changes that accompany tumor progression, termed Tumor Associated Collagen Signatures (TACS). Late stages of this progression are characterized by the presence of bundled, straight collagen (TACS-2) that become oriented perpendicular to the tumor-stromal boundary (TACS-3). Importantly, the presence of TACS-3 collagen is an independent predictor of poor patient outcome. At present, it remains unclear whether reorganization of the collagen matrix is the consequence of mechanical or compositional tissue remodeling. Here, we identify compositional changes in ECM correlating to collagen fiber reorganization from nineteen normal and invasive ductal carcinoma (IDC) patient biopsies using matrisome-targeted proteomics. Twenty-seven ECM proteins were significantly altered in IDC samples compared to normal tissue. Further, a set of nineteen matrisome proteins positively correlate and five proteins inversely correlate with IDC tissues containing straightened collagen fibers. Tenascin-C and thrombospondin-2 significantly co-localized with aligned collagen fibers in IDC tissues. This study highlights the compositional change in matrisome proteins accompanying collagen re-organization during breast cancer progression and provides candidate proteins for investigation into cellular and structural influences on collagen alignment.Lucas A. TomkoRyan C. HillAlexander BarrettJoseph M. SzulczewskiMatthew W. ConklinKevin W. EliceiriPatricia J. KeelyKirk C. HansenSuzanne M. PonikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucas A. Tomko Ryan C. Hill Alexander Barrett Joseph M. Szulczewski Matthew W. Conklin Kevin W. Eliceiri Patricia J. Keely Kirk C. Hansen Suzanne M. Ponik Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
description	Abstract Increasing evidence demonstrates an important role for the extracellular matrix (ECM) in breast cancer progression. Collagen type I, a core constituent of the fibrous ECM, undergoes a significant set of changes that accompany tumor progression, termed Tumor Associated Collagen Signatures (TACS). Late stages of this progression are characterized by the presence of bundled, straight collagen (TACS-2) that become oriented perpendicular to the tumor-stromal boundary (TACS-3). Importantly, the presence of TACS-3 collagen is an independent predictor of poor patient outcome. At present, it remains unclear whether reorganization of the collagen matrix is the consequence of mechanical or compositional tissue remodeling. Here, we identify compositional changes in ECM correlating to collagen fiber reorganization from nineteen normal and invasive ductal carcinoma (IDC) patient biopsies using matrisome-targeted proteomics. Twenty-seven ECM proteins were significantly altered in IDC samples compared to normal tissue. Further, a set of nineteen matrisome proteins positively correlate and five proteins inversely correlate with IDC tissues containing straightened collagen fibers. Tenascin-C and thrombospondin-2 significantly co-localized with aligned collagen fibers in IDC tissues. This study highlights the compositional change in matrisome proteins accompanying collagen re-organization during breast cancer progression and provides candidate proteins for investigation into cellular and structural influences on collagen alignment.
format	article
author	Lucas A. Tomko Ryan C. Hill Alexander Barrett Joseph M. Szulczewski Matthew W. Conklin Kevin W. Eliceiri Patricia J. Keely Kirk C. Hansen Suzanne M. Ponik
author_facet	Lucas A. Tomko Ryan C. Hill Alexander Barrett Joseph M. Szulczewski Matthew W. Conklin Kevin W. Eliceiri Patricia J. Keely Kirk C. Hansen Suzanne M. Ponik
author_sort	Lucas A. Tomko
title	Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
title_short	Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
title_full	Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
title_fullStr	Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
title_full_unstemmed	Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma
title_sort	targeted matrisome analysis identifies thrombospondin-2 and tenascin-c in aligned collagen stroma from invasive breast carcinoma
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/23bb514e9fc84cb880e52835f19ae0f8
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Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma

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