Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.

Reverse genetics in the mosquito Anopheles gambiae by RNAi mediated gene silencing has led in recent years to an advanced understanding of the mosquito immune response against infections with bacteria and malaria parasites. We developed RNAi screens in An. gambiae hemocyte-like cells using a library...

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Autores principales:	Fabrizio Lombardo, Yasmeen Ghani, Fotis C Kafatos, George K Christophides
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/23c55539969d4de6b3676a9dd1b61932
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spelling	oai:doaj.org-article:23c55539969d4de6b3676a9dd1b619322021-11-18T06:06:06ZComprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.1553-73661553-737410.1371/journal.ppat.1003145https://doaj.org/article/23c55539969d4de6b3676a9dd1b619322013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382679/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Reverse genetics in the mosquito Anopheles gambiae by RNAi mediated gene silencing has led in recent years to an advanced understanding of the mosquito immune response against infections with bacteria and malaria parasites. We developed RNAi screens in An. gambiae hemocyte-like cells using a library of double-stranded RNAs targeting 109 genes expressed highly or specifically in mosquito hemocytes to identify novel regulators of the hemocyte immune response. Assays included phagocytosis of bacterial bioparticles, expression of the antimicrobial peptide CEC1, and basal and induced expression of the mosquito complement factor LRIM1. A cell viability screen was also carried out to assess dsRNA cytotoxicity and to identify genes involved in cell growth and survival. Our results identify 22 novel immune regulators, including proteins putatively involved in phagosome assembly and maturation (Ca²⁺ channel, v-ATPase and cyclin-dependent protein kinase), pattern recognition (fibrinogen-domain lectins and Nimrod), immune modulation (peptidase and serine protease homolog), immune signaling (Eiger and LPS-induced factor), cell adhesion and communication (Laminin B1 and Ninjurin) and immune homeostasis (Lipophorin receptor). The development of robust functional cell-based assays paves the way for genome-wide functional screens to study the mosquito immune response to infections with human pathogens.Fabrizio LombardoYasmeen GhaniFotis C KafatosGeorge K ChristophidesPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003145 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Fabrizio Lombardo Yasmeen Ghani Fotis C Kafatos George K Christophides Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
description	Reverse genetics in the mosquito Anopheles gambiae by RNAi mediated gene silencing has led in recent years to an advanced understanding of the mosquito immune response against infections with bacteria and malaria parasites. We developed RNAi screens in An. gambiae hemocyte-like cells using a library of double-stranded RNAs targeting 109 genes expressed highly or specifically in mosquito hemocytes to identify novel regulators of the hemocyte immune response. Assays included phagocytosis of bacterial bioparticles, expression of the antimicrobial peptide CEC1, and basal and induced expression of the mosquito complement factor LRIM1. A cell viability screen was also carried out to assess dsRNA cytotoxicity and to identify genes involved in cell growth and survival. Our results identify 22 novel immune regulators, including proteins putatively involved in phagosome assembly and maturation (Ca²⁺ channel, v-ATPase and cyclin-dependent protein kinase), pattern recognition (fibrinogen-domain lectins and Nimrod), immune modulation (peptidase and serine protease homolog), immune signaling (Eiger and LPS-induced factor), cell adhesion and communication (Laminin B1 and Ninjurin) and immune homeostasis (Lipophorin receptor). The development of robust functional cell-based assays paves the way for genome-wide functional screens to study the mosquito immune response to infections with human pathogens.
format	article
author	Fabrizio Lombardo Yasmeen Ghani Fotis C Kafatos George K Christophides
author_facet	Fabrizio Lombardo Yasmeen Ghani Fotis C Kafatos George K Christophides
author_sort	Fabrizio Lombardo
title	Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
title_short	Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
title_full	Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
title_fullStr	Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
title_full_unstemmed	Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.
title_sort	comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito anopheles gambiae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/23c55539969d4de6b3676a9dd1b61932
work_keys_str_mv	AT fabriziolombardo comprehensivegeneticdissectionofthehemocyteimmuneresponseinthemalariamosquitoanophelesgambiae AT yasmeenghani comprehensivegeneticdissectionofthehemocyteimmuneresponseinthemalariamosquitoanophelesgambiae AT fotisckafatos comprehensivegeneticdissectionofthehemocyteimmuneresponseinthemalariamosquitoanophelesgambiae AT georgekchristophides comprehensivegeneticdissectionofthehemocyteimmuneresponseinthemalariamosquitoanophelesgambiae
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Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae.

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