HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS

Catalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the natur...

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Autores principales:	M. V. Volkova, A. V. Kunder, I. I. Generalov, D. M. Petrovich
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	immunoglobulins abzymes nuclease activity heterogeneity rheumatoid arthritis reactive arthritis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/23c86a4a3c1f49a298d392ffdc493365
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spelling	oai:doaj.org-article:23c86a4a3c1f49a298d392ffdc4933652021-11-18T08:03:46ZHETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS1563-06252313-741X10.15789/1563-0625-2017-6-771-778https://doaj.org/article/23c86a4a3c1f49a298d392ffdc4933652017-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1401https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XCatalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the nature of nuclease activity in these diseases. Determination of DNAse activity of immunoglobulins with different DNA substrates, and search for specific substrates for distinct clinical entities could serve these purposes. The aim of present work is to determine DNase activity of the polyclonal class G immunoglobulins in rheumatoid and reactive arthritis using various methods.Different methods are used to evaluate nuclease activity. In this paper we present newly developed and modified techniques for determination of DNAse activity of polyclonal IgGs. Particular attention was paid to the electrophoretic method of DNase activity assessment. Polyclonal IgG isolated from blood serum of patients with rheumatoid arthritis and reactive arthritis were used for assays. In this study, we demonstrated the presence of an inhomogeneous DNase activity of immunoglobulins in relation to different substrates.Along with calf thymus DNA, we used bacterial plasmid DNA and PCR products based on bacterial gene sequences. Levels of DNase activity by rivanol clot method with calf thymus DNA as substrate proved to be higher in patients with rheumatoid arthritis than the control values (p < 0.01). DNase abzyme activity in patients with rheumatoid arthritis was elevated, as compared to the patients with reactive arthritis (p < 0.01).When examining ability of the IgG to hydrolyze procaryotic DNA (bacterial plasmid DNA and PCR products, based on bacterial genes), we obtained heterogeneous results. Different Ig samples showed varying degrees of DNA hydrolysis. Abzyme hydrolysis of DNA substrates longer than 700 bp was more pronounced, as compared to short DNA substrates (100 base pairs).Conclusions: 1) antibodies having deoxyribonucleic activity are present in serum of patients with rheumatoid arthritis, reactive arthritis; 2)DNase abzymes exhibit heterogeneous ability to hydrolyze various DNA substrates; 3) Abzyme-medicted hydrolysis of DNA substrates longer than 700 base pairs is more pronounced, as compared to a short DNA substrates (100 bp) length.M. V. VolkovaA. V. KunderI. I. GeneralovD. M. PetrovichSPb RAACIarticleimmunoglobulinsabzymesnuclease activityheterogeneityrheumatoid arthritisreactive arthritisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 6, Pp 771-778 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	immunoglobulins abzymes nuclease activity heterogeneity rheumatoid arthritis reactive arthritis Immunologic diseases. Allergy RC581-607
spellingShingle	immunoglobulins abzymes nuclease activity heterogeneity rheumatoid arthritis reactive arthritis Immunologic diseases. Allergy RC581-607 M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
description	Catalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the nature of nuclease activity in these diseases. Determination of DNAse activity of immunoglobulins with different DNA substrates, and search for specific substrates for distinct clinical entities could serve these purposes. The aim of present work is to determine DNase activity of the polyclonal class G immunoglobulins in rheumatoid and reactive arthritis using various methods.Different methods are used to evaluate nuclease activity. In this paper we present newly developed and modified techniques for determination of DNAse activity of polyclonal IgGs. Particular attention was paid to the electrophoretic method of DNase activity assessment. Polyclonal IgG isolated from blood serum of patients with rheumatoid arthritis and reactive arthritis were used for assays. In this study, we demonstrated the presence of an inhomogeneous DNase activity of immunoglobulins in relation to different substrates.Along with calf thymus DNA, we used bacterial plasmid DNA and PCR products based on bacterial gene sequences. Levels of DNase activity by rivanol clot method with calf thymus DNA as substrate proved to be higher in patients with rheumatoid arthritis than the control values (p < 0.01). DNase abzyme activity in patients with rheumatoid arthritis was elevated, as compared to the patients with reactive arthritis (p < 0.01).When examining ability of the IgG to hydrolyze procaryotic DNA (bacterial plasmid DNA and PCR products, based on bacterial genes), we obtained heterogeneous results. Different Ig samples showed varying degrees of DNA hydrolysis. Abzyme hydrolysis of DNA substrates longer than 700 bp was more pronounced, as compared to short DNA substrates (100 base pairs).Conclusions: 1) antibodies having deoxyribonucleic activity are present in serum of patients with rheumatoid arthritis, reactive arthritis; 2)DNase abzymes exhibit heterogeneous ability to hydrolyze various DNA substrates; 3) Abzyme-medicted hydrolysis of DNA substrates longer than 700 base pairs is more pronounced, as compared to a short DNA substrates (100 bp) length.
format	article
author	M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich
author_facet	M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich
author_sort	M. V. Volkova
title	HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
title_short	HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
title_full	HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
title_fullStr	HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
title_full_unstemmed	HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
title_sort	heterogeneity of polyclonal immunoglobulins nuclease activity in rheumatoid and reactive arthritis
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/23c86a4a3c1f49a298d392ffdc493365
work_keys_str_mv	AT mvvolkova heterogeneityofpolyclonalimmunoglobulinsnucleaseactivityinrheumatoidandreactivearthritis AT avkunder heterogeneityofpolyclonalimmunoglobulinsnucleaseactivityinrheumatoidandreactivearthritis AT iigeneralov heterogeneityofpolyclonalimmunoglobulinsnucleaseactivityinrheumatoidandreactivearthritis AT dmpetrovich heterogeneityofpolyclonalimmunoglobulinsnucleaseactivityinrheumatoidandreactivearthritis
_version_	1718422402787966976

HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS

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