Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.

<h4>Background</h4>Multiple sclerosis (MS) and neuromyelitis optica (NMO) occasionally have an extremely aggressive and debilitating disease course; however, its molecular basis is unknown. This study aimed to determine a relationship between connexin (Cx) pathology and disease aggressiv...

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Autores principales:	Katsuhisa Masaki, Satoshi O Suzuki, Takuya Matsushita, Takeshi Matsuoka, Shihoko Imamura, Ryo Yamasaki, Makiko Suzuki, Toshihiko Suenaga, Toru Iwaki, Jun-Ichi Kira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23d902ac90ca4ff084b8d8f5515524ec
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spelling	oai:doaj.org-article:23d902ac90ca4ff084b8d8f5515524ec2021-11-18T08:58:25ZConnexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.1932-620310.1371/journal.pone.0072919https://doaj.org/article/23d902ac90ca4ff084b8d8f5515524ec2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23991165/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Multiple sclerosis (MS) and neuromyelitis optica (NMO) occasionally have an extremely aggressive and debilitating disease course; however, its molecular basis is unknown. This study aimed to determine a relationship between connexin (Cx) pathology and disease aggressiveness in Asian patients with MS and NMO.<h4>Methods/principal findings</h4>Samples included 11 autopsied cases with NMO and NMO spectrum disorder (NMOSD), six with MS, and 20 with other neurological diseases (OND). Methods of analysis included immunohistochemical expression of astrocytic Cx43/Cx30, oligodendrocytic Cx47/Cx32 relative to AQP4 and other astrocytic and oligodendrocytic proteins, extent of demyelination, the vasculocentric deposition of complement and immunoglobulin, and lesion staging by CD68 staining for macrophages. Lesions were classified as actively demyelinating (n=59), chronic active (n=58) and chronic inactive (n=23). Sera from 120 subjects including 30 MS, 30 NMO, 40 OND and 20 healthy controls were examined for anti-Cx43 antibody by cell-based assay. Six NMO/NMOSD and three MS cases showed preferential loss of astrocytic Cx43 beyond the demyelinated areas in actively demyelinating and chronic active lesions, where heterotypic Cx43/Cx47 astrocyte oligodendrocyte gap junctions were extensively lost. Cx43 loss was significantly associated with a rapidly progressive disease course as six of nine cases with Cx43 loss, but none of eight cases without Cx43 loss regardless of disease phenotype, died within two years after disease onset (66.7% vs. 0%, P=0.0090). Overall, five of nine cases with Cx43 loss and none of eight cases without Cx43 loss had distal oligodendrogliopathy characterized by selective myelin associated glycoprotein loss (55.6% vs. 0.0%, P=0.0296). Loss of oligodendrocytic Cx32 and Cx47 expression was observed in most active and chronic lesions from all MS and NMO/NMOSD cases. Cx43-specific antibodies were absent in NMO/NMOSD and MS patients.<h4>Conclusions</h4>These findings suggest that autoantibody-independent astrocytic Cx43 loss may relate to disease aggressiveness and distal oligodendrogliopathy in both MS and NMO.Katsuhisa MasakiSatoshi O SuzukiTakuya MatsushitaTakeshi MatsuokaShihoko ImamuraRyo YamasakiMakiko SuzukiToshihiko SuenagaToru IwakiJun-Ichi KiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72919 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katsuhisa Masaki Satoshi O Suzuki Takuya Matsushita Takeshi Matsuoka Shihoko Imamura Ryo Yamasaki Makiko Suzuki Toshihiko Suenaga Toru Iwaki Jun-Ichi Kira Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
description	<h4>Background</h4>Multiple sclerosis (MS) and neuromyelitis optica (NMO) occasionally have an extremely aggressive and debilitating disease course; however, its molecular basis is unknown. This study aimed to determine a relationship between connexin (Cx) pathology and disease aggressiveness in Asian patients with MS and NMO.<h4>Methods/principal findings</h4>Samples included 11 autopsied cases with NMO and NMO spectrum disorder (NMOSD), six with MS, and 20 with other neurological diseases (OND). Methods of analysis included immunohistochemical expression of astrocytic Cx43/Cx30, oligodendrocytic Cx47/Cx32 relative to AQP4 and other astrocytic and oligodendrocytic proteins, extent of demyelination, the vasculocentric deposition of complement and immunoglobulin, and lesion staging by CD68 staining for macrophages. Lesions were classified as actively demyelinating (n=59), chronic active (n=58) and chronic inactive (n=23). Sera from 120 subjects including 30 MS, 30 NMO, 40 OND and 20 healthy controls were examined for anti-Cx43 antibody by cell-based assay. Six NMO/NMOSD and three MS cases showed preferential loss of astrocytic Cx43 beyond the demyelinated areas in actively demyelinating and chronic active lesions, where heterotypic Cx43/Cx47 astrocyte oligodendrocyte gap junctions were extensively lost. Cx43 loss was significantly associated with a rapidly progressive disease course as six of nine cases with Cx43 loss, but none of eight cases without Cx43 loss regardless of disease phenotype, died within two years after disease onset (66.7% vs. 0%, P=0.0090). Overall, five of nine cases with Cx43 loss and none of eight cases without Cx43 loss had distal oligodendrogliopathy characterized by selective myelin associated glycoprotein loss (55.6% vs. 0.0%, P=0.0296). Loss of oligodendrocytic Cx32 and Cx47 expression was observed in most active and chronic lesions from all MS and NMO/NMOSD cases. Cx43-specific antibodies were absent in NMO/NMOSD and MS patients.<h4>Conclusions</h4>These findings suggest that autoantibody-independent astrocytic Cx43 loss may relate to disease aggressiveness and distal oligodendrogliopathy in both MS and NMO.
format	article
author	Katsuhisa Masaki Satoshi O Suzuki Takuya Matsushita Takeshi Matsuoka Shihoko Imamura Ryo Yamasaki Makiko Suzuki Toshihiko Suenaga Toru Iwaki Jun-Ichi Kira
author_facet	Katsuhisa Masaki Satoshi O Suzuki Takuya Matsushita Takeshi Matsuoka Shihoko Imamura Ryo Yamasaki Makiko Suzuki Toshihiko Suenaga Toru Iwaki Jun-Ichi Kira
author_sort	Katsuhisa Masaki
title	Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
title_short	Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
title_full	Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
title_fullStr	Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
title_full_unstemmed	Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
title_sort	connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/23d902ac90ca4ff084b8d8f5515524ec
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Connexin 43 astrocytopathy linked to rapidly progressive multiple sclerosis and neuromyelitis optica.

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