Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal

The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among <i>Escherichia coli</i> producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected an...

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Autores principales:	Lurdes Clemente, Célia Leão, Laura Moura, Teresa Albuquerque, Ana Amaro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antimicrobial resistance <i>Escherichia coli</i> meat ESBL/AmpC <i>mcr</i> genes PMQR Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/23e011e878894df19c122ef090afb90f
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spelling	oai:doaj.org-article:23e011e878894df19c122ef090afb90f2021-11-25T16:22:58ZPrevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal10.3390/antibiotics101113332079-6382https://doaj.org/article/23e011e878894df19c122ef090afb90f2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1333https://doaj.org/toc/2079-6382The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among <i>Escherichia coli</i> producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected and inoculated on selective medium for the search of <i>E. coli</i> resistant to 3rd generation cephalosporins. Isolates were characterized by antimicrobial susceptibility testing, molecular assays targeting ESBL/AmpC, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated colistin resistance (PMCR) encoding genes. The highest frequency of <i>E. coli</i> non-wild type to 3rd generation cephalosporins and fluoroquinolones was observed in broiler meat (30.3% and 93.3%, respectively). Overall, a diversity of acquired resistance mechanisms, were detected: <i>bla</i><sub>ESBL</sub> [<i>bla</i><sub>CTX-M-1</sub> (<i>n</i> = 19), <i>bla</i><sub>CTX-M-15</sub> (<i>n</i> = 4), <i>bla</i><sub>CTX-M-32</sub> (<i>n</i> = 12), <i>bla</i><sub>CTX-M-55</sub> (<i>n</i> = 8), <i>bla</i><sub>CTX-M-65</sub> (<i>n</i> = 4), <i>bla</i><sub>CTX-M-27</sub> (<i>n</i> = 2), <i>bla</i><sub>CTX-M-9</sub> (<i>n</i> = 1), <i>bla</i><sub>CTX-M-14</sub> (<i>n</i> = 11), <i>bla</i><sub>SHV-12</sub> (<i>n</i> = 27), <i>bla</i><sub>TEM-52</sub> (<i>n</i> = 1)], <i>bla</i><sub>PMAβ</sub> [<i>bla</i><sub>CMY-2</sub> (<i>n</i> = 8)], PMQR [<i>qnrB</i> (<i>n</i> = 27), <i>qnrS</i> (<i>n</i> = 21) and <i>aac(6’)-Ib-type</i> (<i>n</i> = 4)] and PMCR [<i>mcr-1</i> (<i>n</i> = 8)]. Our study highlights that consumers may be exposed through the food chain to multidrug-resistant <i>E. coli</i> carrying diverse plasmid-mediated antimicrobial resistance genes, posing a great hazard to food safety and a public health risk.Lurdes ClementeCélia LeãoLaura MouraTeresa AlbuquerqueAna AmaroMDPI AGarticleantimicrobial resistance<i>Escherichia coli</i>meatESBL/AmpC<i>mcr</i> genesPMQRTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1333, p 1333 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial resistance <i>Escherichia coli</i> meat ESBL/AmpC <i>mcr</i> genes PMQR Therapeutics. Pharmacology RM1-950
spellingShingle	antimicrobial resistance <i>Escherichia coli</i> meat ESBL/AmpC <i>mcr</i> genes PMQR Therapeutics. Pharmacology RM1-950 Lurdes Clemente Célia Leão Laura Moura Teresa Albuquerque Ana Amaro Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
description	The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among <i>Escherichia coli</i> producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected and inoculated on selective medium for the search of <i>E. coli</i> resistant to 3rd generation cephalosporins. Isolates were characterized by antimicrobial susceptibility testing, molecular assays targeting ESBL/AmpC, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated colistin resistance (PMCR) encoding genes. The highest frequency of <i>E. coli</i> non-wild type to 3rd generation cephalosporins and fluoroquinolones was observed in broiler meat (30.3% and 93.3%, respectively). Overall, a diversity of acquired resistance mechanisms, were detected: <i>bla</i><sub>ESBL</sub> [<i>bla</i><sub>CTX-M-1</sub> (<i>n</i> = 19), <i>bla</i><sub>CTX-M-15</sub> (<i>n</i> = 4), <i>bla</i><sub>CTX-M-32</sub> (<i>n</i> = 12), <i>bla</i><sub>CTX-M-55</sub> (<i>n</i> = 8), <i>bla</i><sub>CTX-M-65</sub> (<i>n</i> = 4), <i>bla</i><sub>CTX-M-27</sub> (<i>n</i> = 2), <i>bla</i><sub>CTX-M-9</sub> (<i>n</i> = 1), <i>bla</i><sub>CTX-M-14</sub> (<i>n</i> = 11), <i>bla</i><sub>SHV-12</sub> (<i>n</i> = 27), <i>bla</i><sub>TEM-52</sub> (<i>n</i> = 1)], <i>bla</i><sub>PMAβ</sub> [<i>bla</i><sub>CMY-2</sub> (<i>n</i> = 8)], PMQR [<i>qnrB</i> (<i>n</i> = 27), <i>qnrS</i> (<i>n</i> = 21) and <i>aac(6’)-Ib-type</i> (<i>n</i> = 4)] and PMCR [<i>mcr-1</i> (<i>n</i> = 8)]. Our study highlights that consumers may be exposed through the food chain to multidrug-resistant <i>E. coli</i> carrying diverse plasmid-mediated antimicrobial resistance genes, posing a great hazard to food safety and a public health risk.
format	article
author	Lurdes Clemente Célia Leão Laura Moura Teresa Albuquerque Ana Amaro
author_facet	Lurdes Clemente Célia Leão Laura Moura Teresa Albuquerque Ana Amaro
author_sort	Lurdes Clemente
title	Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
title_short	Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
title_full	Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
title_fullStr	Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
title_full_unstemmed	Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal
title_sort	prevalence and characterization of esbl/ampc producing <i>escherichia coli</i> from fresh meat in portugal
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/23e011e878894df19c122ef090afb90f
work_keys_str_mv	AT lurdesclemente prevalenceandcharacterizationofesblampcproducingiescherichiacoliifromfreshmeatinportugal AT celialeao prevalenceandcharacterizationofesblampcproducingiescherichiacoliifromfreshmeatinportugal AT lauramoura prevalenceandcharacterizationofesblampcproducingiescherichiacoliifromfreshmeatinportugal AT teresaalbuquerque prevalenceandcharacterizationofesblampcproducingiescherichiacoliifromfreshmeatinportugal AT anaamaro prevalenceandcharacterizationofesblampcproducingiescherichiacoliifromfreshmeatinportugal
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Prevalence and Characterization of ESBL/AmpC Producing <i>Escherichia coli</i> from Fresh Meat in Portugal

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