Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP

ABSTRACT In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demons...

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Autores principales:	Iwona B. Wenderska, Andrew Latos, Benjamin Pruitt, Sara Palmer, Grace Spatafora, Dilani B. Senadheera, Dennis G. Cvitkovitch
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	ComRS Streptococcus mutans XIP cell signaling genetic competence signaling peptides Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/23e1c7c0695146cdb0914218a4aab703
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spelling	oai:doaj.org-article:23e1c7c0695146cdb0914218a4aab7032021-12-02T19:47:35ZTranscriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP10.1128/mSystems.00102-162379-5077https://doaj.org/article/23e1c7c0695146cdb0914218a4aab7032017-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00102-16https://doaj.org/toc/2379-5077ABSTRACT In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S. mutans transcriptome. RNA sequencing revealed that XIP induction resulted in a global change in gene expression that was consistent with a stress response. An increase in several membrane-bound regulators, including HdrRM and BrsRM, involved in bacteriocin production, and the VicRKX system, involved in acid tolerance and biofilm formation, was observed. Furthermore, global changes in gene expression corresponded to changes observed during the stringent response to amino acid starvation. Effects were also observed on genes involved in sugar transport and carbon catabolite repression and included the levQRST and levDEFG operons. Finally, our work identified a novel heat shock-responsive intergenic region, encoding a small RNA, with a potential role in competence shutoff. IMPORTANCE Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a survival advantage. In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (ComX-inducing peptide). The present study utilizes high-throughput RNA sequencing (RNAseq) to provide a greater understanding of how global gene expression patterns change in response to XIP. Overall, our work demonstrates that in S. mutans, XIP signaling induces a response that resembles the stringent response to amino acid starvation. We further identify a novel heat shock-responsive intergenic region with a potential role in competence shutoff. Together, our results provide further evidence that multiple stress response mechanisms are linked through the genetic competence signaling pathway in S. mutans.Iwona B. WenderskaAndrew LatosBenjamin PruittSara PalmerGrace SpataforaDilani B. SenadheeraDennis G. CvitkovitchAmerican Society for MicrobiologyarticleComRSStreptococcus mutansXIPcell signalinggenetic competencesignaling peptidesMicrobiologyQR1-502ENmSystems, Vol 2, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	ComRS Streptococcus mutans XIP cell signaling genetic competence signaling peptides Microbiology QR1-502
spellingShingle	ComRS Streptococcus mutans XIP cell signaling genetic competence signaling peptides Microbiology QR1-502 Iwona B. Wenderska Andrew Latos Benjamin Pruitt Sara Palmer Grace Spatafora Dilani B. Senadheera Dennis G. Cvitkovitch Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
description	ABSTRACT In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S. mutans transcriptome. RNA sequencing revealed that XIP induction resulted in a global change in gene expression that was consistent with a stress response. An increase in several membrane-bound regulators, including HdrRM and BrsRM, involved in bacteriocin production, and the VicRKX system, involved in acid tolerance and biofilm formation, was observed. Furthermore, global changes in gene expression corresponded to changes observed during the stringent response to amino acid starvation. Effects were also observed on genes involved in sugar transport and carbon catabolite repression and included the levQRST and levDEFG operons. Finally, our work identified a novel heat shock-responsive intergenic region, encoding a small RNA, with a potential role in competence shutoff. IMPORTANCE Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a survival advantage. In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (ComX-inducing peptide). The present study utilizes high-throughput RNA sequencing (RNAseq) to provide a greater understanding of how global gene expression patterns change in response to XIP. Overall, our work demonstrates that in S. mutans, XIP signaling induces a response that resembles the stringent response to amino acid starvation. We further identify a novel heat shock-responsive intergenic region with a potential role in competence shutoff. Together, our results provide further evidence that multiple stress response mechanisms are linked through the genetic competence signaling pathway in S. mutans.
format	article
author	Iwona B. Wenderska Andrew Latos Benjamin Pruitt Sara Palmer Grace Spatafora Dilani B. Senadheera Dennis G. Cvitkovitch
author_facet	Iwona B. Wenderska Andrew Latos Benjamin Pruitt Sara Palmer Grace Spatafora Dilani B. Senadheera Dennis G. Cvitkovitch
author_sort	Iwona B. Wenderska
title	Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
title_short	Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
title_full	Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
title_fullStr	Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
title_full_unstemmed	Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP
title_sort	transcriptional profiling of the oral pathogen <named-content content-type="genus-species">streptococcus mutans</named-content> in response to competence signaling peptide xip
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/23e1c7c0695146cdb0914218a4aab703
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Transcriptional Profiling of the Oral Pathogen <named-content content-type="genus-species">Streptococcus mutans</named-content> in Response to Competence Signaling Peptide XIP

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