A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice

Abstract A GalNAc/Gal-specific lectin (CGL) from the edible mussel Crenomytilus grayanus has been demonstrated to exhibit antibacterial properties. However, the mechanism of immune modulation by CGL in mammalian cells remains unclear. Here, we demonstrated that CGL can activate immune responses in m...

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Main Authors:	Oleg V. Chernikov, Wei-Ting Wong, Lan-Hui Li, Irina V. Chikalovets, Valentina I. Molchanova, Shih-Hsiung Wu, Jiahn-Haur Liao, Kuo-Feng Hua
Format:	article
Language:	EN
Published:	Nature Portfolio 2017
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/23e3ea93929e40c1a607fc0ae4e865a8
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spelling	oai:doaj.org-article:23e3ea93929e40c1a607fc0ae4e865a82021-12-02T12:30:27ZA GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice10.1038/s41598-017-06647-52045-2322https://doaj.org/article/23e3ea93929e40c1a607fc0ae4e865a82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06647-5https://doaj.org/toc/2045-2322Abstract A GalNAc/Gal-specific lectin (CGL) from the edible mussel Crenomytilus grayanus has been demonstrated to exhibit antibacterial properties. However, the mechanism of immune modulation by CGL in mammalian cells remains unclear. Here, we demonstrated that CGL can activate immune responses in macrophages and in mice. In the in vitro cell models, CGL induced tumour necrosis factor-α and interleukin-6 secretion in mouse RAW264.7 macrophages, mouse bone marrow-derived macrophages, human THP-1 macrophages, human peripheral blood mononuclear cells and human blood monocyte-derived macrophages. The CGL-mediated cytokine production was regulated by reactive oxygen species, mitogen-activated protein kinases, protein kinase C-α/δ and NF-κB. Interestingly, in lipopolysaccharide-activated macrophages, CGL induced endotoxin tolerance (characterized by the downregulation of nitric oxide, inducible nitric oxide synthase, interleukin-6 and cyclooxygenase II) via the downregulation of IRAK2 expression, JNK1/2 phosphorylation and NF-κB activation. CGL also slightly increased the bactericidal activity of macrophages and induced cytokine production in mouse models. Overall, our data indicate that CGL has the potential to be used as an immune modulator in mammals.Oleg V. ChernikovWei-Ting WongLan-Hui LiIrina V. ChikalovetsValentina I. MolchanovaShih-Hsiung WuJiahn-Haur LiaoKuo-Feng HuaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oleg V. Chernikov Wei-Ting Wong Lan-Hui Li Irina V. Chikalovets Valentina I. Molchanova Shih-Hsiung Wu Jiahn-Haur Liao Kuo-Feng Hua A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
description	Abstract A GalNAc/Gal-specific lectin (CGL) from the edible mussel Crenomytilus grayanus has been demonstrated to exhibit antibacterial properties. However, the mechanism of immune modulation by CGL in mammalian cells remains unclear. Here, we demonstrated that CGL can activate immune responses in macrophages and in mice. In the in vitro cell models, CGL induced tumour necrosis factor-α and interleukin-6 secretion in mouse RAW264.7 macrophages, mouse bone marrow-derived macrophages, human THP-1 macrophages, human peripheral blood mononuclear cells and human blood monocyte-derived macrophages. The CGL-mediated cytokine production was regulated by reactive oxygen species, mitogen-activated protein kinases, protein kinase C-α/δ and NF-κB. Interestingly, in lipopolysaccharide-activated macrophages, CGL induced endotoxin tolerance (characterized by the downregulation of nitric oxide, inducible nitric oxide synthase, interleukin-6 and cyclooxygenase II) via the downregulation of IRAK2 expression, JNK1/2 phosphorylation and NF-κB activation. CGL also slightly increased the bactericidal activity of macrophages and induced cytokine production in mouse models. Overall, our data indicate that CGL has the potential to be used as an immune modulator in mammals.
format	article
author	Oleg V. Chernikov Wei-Ting Wong Lan-Hui Li Irina V. Chikalovets Valentina I. Molchanova Shih-Hsiung Wu Jiahn-Haur Liao Kuo-Feng Hua
author_facet	Oleg V. Chernikov Wei-Ting Wong Lan-Hui Li Irina V. Chikalovets Valentina I. Molchanova Shih-Hsiung Wu Jiahn-Haur Liao Kuo-Feng Hua
author_sort	Oleg V. Chernikov
title	A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
title_short	A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
title_full	A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
title_fullStr	A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
title_full_unstemmed	A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice
title_sort	galnac/gal-specific lectin from the sea mussel crenomytilus grayanus modulates immune response in macrophages and in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/23e3ea93929e40c1a607fc0ae4e865a8
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A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice

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