Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice

Abstract Segmental duplication is a major structural variation that occurs in chromosomes. Duplication leads to the production of gene copies with increased numbers of related repeat segments, causing the global genome to be in a state of imbalance. In addition, if the added segment contains a centr...

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Autores principales:	Rong Zhang, Chao Xue, Guanqing Liu, Xiaoyu Liu, Mingliang Zhang, Xiao Wang, Tao Zhang, Zhiyun Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23e94d49b0c349be9d05f1fc3690f0e2
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spelling	oai:doaj.org-article:23e94d49b0c349be9d05f1fc3690f0e22021-12-02T11:40:41ZSegmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice10.1038/s41598-017-02796-92045-2322https://doaj.org/article/23e94d49b0c349be9d05f1fc3690f0e22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02796-9https://doaj.org/toc/2045-2322Abstract Segmental duplication is a major structural variation that occurs in chromosomes. Duplication leads to the production of gene copies with increased numbers of related repeat segments, causing the global genome to be in a state of imbalance. In addition, if the added segment contains a centromeric specific DNA, the duplicated chromosome will have structural multiple centromeres. We identified a segmental duplication containing structurally tricentric regions derived from the short arm of chromosome 11 (11L∙ + 11L∙ + 11S∙11S∙11S∙11S, “∙” represents the centromeric DNA repeat loci), and analyzed its implications for cell division and genome-wide expression. In the variant, only the middle centromere of 11S∙11S∙11S∙11S is functionally active. As a result, the structurally tricentric chromosome was stable in mitosis, because it is actually a functional monocentric chromosome. However, the structurally tricentric chromosome, which usually formed a bivalent, was either arranged on the equatorial plane or was lagging, which affected its separation during meiosis. Furthermore, RNA-seq and RT-qPCR analysis showed that the segmental duplication affected genome-wide expression patterns. 34.60% of genes in repeat region showed positive dosage effect. Thus, the genes on chromosome arm 11S-2 didn’t exhibit obviously dosage compensation, as illustrated by no peak around a ratio of 1.00. However, the gene dosage effect will reduce after sexual reproduction of a generation.Rong ZhangChao XueGuanqing LiuXiaoyu LiuMingliang ZhangXiao WangTao ZhangZhiyun GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Zhang Chao Xue Guanqing Liu Xiaoyu Liu Mingliang Zhang Xiao Wang Tao Zhang Zhiyun Gong Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
description	Abstract Segmental duplication is a major structural variation that occurs in chromosomes. Duplication leads to the production of gene copies with increased numbers of related repeat segments, causing the global genome to be in a state of imbalance. In addition, if the added segment contains a centromeric specific DNA, the duplicated chromosome will have structural multiple centromeres. We identified a segmental duplication containing structurally tricentric regions derived from the short arm of chromosome 11 (11L∙ + 11L∙ + 11S∙11S∙11S∙11S, “∙” represents the centromeric DNA repeat loci), and analyzed its implications for cell division and genome-wide expression. In the variant, only the middle centromere of 11S∙11S∙11S∙11S is functionally active. As a result, the structurally tricentric chromosome was stable in mitosis, because it is actually a functional monocentric chromosome. However, the structurally tricentric chromosome, which usually formed a bivalent, was either arranged on the equatorial plane or was lagging, which affected its separation during meiosis. Furthermore, RNA-seq and RT-qPCR analysis showed that the segmental duplication affected genome-wide expression patterns. 34.60% of genes in repeat region showed positive dosage effect. Thus, the genes on chromosome arm 11S-2 didn’t exhibit obviously dosage compensation, as illustrated by no peak around a ratio of 1.00. However, the gene dosage effect will reduce after sexual reproduction of a generation.
format	article
author	Rong Zhang Chao Xue Guanqing Liu Xiaoyu Liu Mingliang Zhang Xiao Wang Tao Zhang Zhiyun Gong
author_facet	Rong Zhang Chao Xue Guanqing Liu Xiaoyu Liu Mingliang Zhang Xiao Wang Tao Zhang Zhiyun Gong
author_sort	Rong Zhang
title	Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
title_short	Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
title_full	Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
title_fullStr	Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
title_full_unstemmed	Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice
title_sort	segmental duplication of chromosome 11 and its implications for cell division and genome-wide expression in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/23e94d49b0c349be9d05f1fc3690f0e2
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Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice

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