mBeRFP, an improved large stokes shift red fluorescent protein.

Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSS-mKate2), mBeRFP is approximately three times brighter. In add...

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Autores principales:	Jie Yang, Liang Wang, Fei Yang, Haiming Luo, Lingling Xu, Jinling Lu, Shaoqun Zeng, Zhihong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23e9e7e53b514a48ac6ee9f535399958
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spelling	oai:doaj.org-article:23e9e7e53b514a48ac6ee9f5353999582021-11-18T07:40:53ZmBeRFP, an improved large stokes shift red fluorescent protein.1932-620310.1371/journal.pone.0064849https://doaj.org/article/23e9e7e53b514a48ac6ee9f5353999582013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840310/?tool=EBIhttps://doaj.org/toc/1932-6203Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSS-mKate2), mBeRFP is approximately three times brighter. In addition, mBeRFP is characterized by improved photostability, rapid maturation, an extended lifetime, and a monomeric nature. Additionally, mBeRFP can be paired with the Alexa 647 dye as a FRET donor to detect caspase 3 activity. This FRET pair has an extremely dynamic range and a large Förster radius (approximately 6.5 nm). To demonstrate the applicability of mBeRFP for imaging in living cells, we performed dual-color imaging of mBeRFP and CFP simultaneously excited by a single excitation source, and we demonstrated that these fluorescent proteins allow the clear visualization of the dynamics of Bax during cancer cell apoptosis. Thus, mBeRFP appears to be particularly useful for cellular imaging applications.Jie YangLiang WangFei YangHaiming LuoLingling XuJinling LuShaoqun ZengZhihong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e64849 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie Yang Liang Wang Fei Yang Haiming Luo Lingling Xu Jinling Lu Shaoqun Zeng Zhihong Zhang mBeRFP, an improved large stokes shift red fluorescent protein.
description	Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSS-mKate2), mBeRFP is approximately three times brighter. In addition, mBeRFP is characterized by improved photostability, rapid maturation, an extended lifetime, and a monomeric nature. Additionally, mBeRFP can be paired with the Alexa 647 dye as a FRET donor to detect caspase 3 activity. This FRET pair has an extremely dynamic range and a large Förster radius (approximately 6.5 nm). To demonstrate the applicability of mBeRFP for imaging in living cells, we performed dual-color imaging of mBeRFP and CFP simultaneously excited by a single excitation source, and we demonstrated that these fluorescent proteins allow the clear visualization of the dynamics of Bax during cancer cell apoptosis. Thus, mBeRFP appears to be particularly useful for cellular imaging applications.
format	article
author	Jie Yang Liang Wang Fei Yang Haiming Luo Lingling Xu Jinling Lu Shaoqun Zeng Zhihong Zhang
author_facet	Jie Yang Liang Wang Fei Yang Haiming Luo Lingling Xu Jinling Lu Shaoqun Zeng Zhihong Zhang
author_sort	Jie Yang
title	mBeRFP, an improved large stokes shift red fluorescent protein.
title_short	mBeRFP, an improved large stokes shift red fluorescent protein.
title_full	mBeRFP, an improved large stokes shift red fluorescent protein.
title_fullStr	mBeRFP, an improved large stokes shift red fluorescent protein.
title_full_unstemmed	mBeRFP, an improved large stokes shift red fluorescent protein.
title_sort	mberfp, an improved large stokes shift red fluorescent protein.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/23e9e7e53b514a48ac6ee9f535399958
work_keys_str_mv	AT jieyang mberfpanimprovedlargestokesshiftredfluorescentprotein AT liangwang mberfpanimprovedlargestokesshiftredfluorescentprotein AT feiyang mberfpanimprovedlargestokesshiftredfluorescentprotein AT haimingluo mberfpanimprovedlargestokesshiftredfluorescentprotein AT linglingxu mberfpanimprovedlargestokesshiftredfluorescentprotein AT jinlinglu mberfpanimprovedlargestokesshiftredfluorescentprotein AT shaoqunzeng mberfpanimprovedlargestokesshiftredfluorescentprotein AT zhihongzhang mberfpanimprovedlargestokesshiftredfluorescentprotein
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mBeRFP, an improved large stokes shift red fluorescent protein.

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