Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines

Liver cancer cell lines are frequently used in vitro tools to test candidate anti-cancer agents as well as to elucidate mechanisms of liver carcinogenesis. Among such mechanisms is cellular communication mediated by connexin-based gap junctions. The present study investigated changes in connexin exp...

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Autores principales:	Kaat Leroy, Cícero Júlio Silva Costa, Alanah Pieters, Bruna dos Santos Rodrigues, Raf Van Campenhout, Axelle Cooreman, Andrés Tabernilla, Bruno Cogliati, Mathieu Vinken
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	connexin gap junction liver cancer cell line in vitro Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/23ea5ce4b700442b83ea081ef8558eb3
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spelling	oai:doaj.org-article:23ea5ce4b700442b83ea081ef8558eb32021-11-25T17:54:08ZExpression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines10.3390/ijms2222121871422-00671661-6596https://doaj.org/article/23ea5ce4b700442b83ea081ef8558eb32021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12187https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Liver cancer cell lines are frequently used in vitro tools to test candidate anti-cancer agents as well as to elucidate mechanisms of liver carcinogenesis. Among such mechanisms is cellular communication mediated by connexin-based gap junctions. The present study investigated changes in connexin expression and gap junction functionality in liver cancer in vitro. For this purpose, seven human liver cancer cell lines, as well as primary human hepatocytes, were subjected to connexin and gap junction analysis at the transcriptional, translational and activity level. Real-time quantitative reverse transcription polymerase chain reaction analysis showed enhanced expression of connexin43 in the majority of liver cancer cell lines at the expense of connexin32 and connexin26. Some of these changes were paralleled at the protein level, as evidenced by immunoblot analysis and in situ immunocytochemistry. Gap junctional intercellular communication, assessed by the scrape loading/dye transfer assay, was generally low in all liver cancer cell lines. Collectively, these results provide a full scenario of modifications in hepatocyte connexin production and gap junction activity in cultured liver cancer cell lines. The findings may be valuable for the selection of neoplastic hepatocytes for future mechanistic investigation and testing of anti-cancer drugs that target connexins and their channels.Kaat LeroyCícero Júlio Silva CostaAlanah PietersBruna dos Santos RodriguesRaf Van CampenhoutAxelle CooremanAndrés TabernillaBruno CogliatiMathieu VinkenMDPI AGarticleconnexingap junctionliver cancercell linein vitroBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12187, p 12187 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	connexin gap junction liver cancer cell line in vitro Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	connexin gap junction liver cancer cell line in vitro Biology (General) QH301-705.5 Chemistry QD1-999 Kaat Leroy Cícero Júlio Silva Costa Alanah Pieters Bruna dos Santos Rodrigues Raf Van Campenhout Axelle Cooreman Andrés Tabernilla Bruno Cogliati Mathieu Vinken Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
description	Liver cancer cell lines are frequently used in vitro tools to test candidate anti-cancer agents as well as to elucidate mechanisms of liver carcinogenesis. Among such mechanisms is cellular communication mediated by connexin-based gap junctions. The present study investigated changes in connexin expression and gap junction functionality in liver cancer in vitro. For this purpose, seven human liver cancer cell lines, as well as primary human hepatocytes, were subjected to connexin and gap junction analysis at the transcriptional, translational and activity level. Real-time quantitative reverse transcription polymerase chain reaction analysis showed enhanced expression of connexin43 in the majority of liver cancer cell lines at the expense of connexin32 and connexin26. Some of these changes were paralleled at the protein level, as evidenced by immunoblot analysis and in situ immunocytochemistry. Gap junctional intercellular communication, assessed by the scrape loading/dye transfer assay, was generally low in all liver cancer cell lines. Collectively, these results provide a full scenario of modifications in hepatocyte connexin production and gap junction activity in cultured liver cancer cell lines. The findings may be valuable for the selection of neoplastic hepatocytes for future mechanistic investigation and testing of anti-cancer drugs that target connexins and their channels.
format	article
author	Kaat Leroy Cícero Júlio Silva Costa Alanah Pieters Bruna dos Santos Rodrigues Raf Van Campenhout Axelle Cooreman Andrés Tabernilla Bruno Cogliati Mathieu Vinken
author_facet	Kaat Leroy Cícero Júlio Silva Costa Alanah Pieters Bruna dos Santos Rodrigues Raf Van Campenhout Axelle Cooreman Andrés Tabernilla Bruno Cogliati Mathieu Vinken
author_sort	Kaat Leroy
title	Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
title_short	Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
title_full	Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
title_fullStr	Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
title_full_unstemmed	Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines
title_sort	expression and functionality of connexin-based channels in human liver cancer cell lines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/23ea5ce4b700442b83ea081ef8558eb3
work_keys_str_mv	AT kaatleroy expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT cicerojuliosilvacosta expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT alanahpieters expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT brunadossantosrodrigues expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT rafvancampenhout expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT axellecooreman expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT andrestabernilla expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT brunocogliati expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines AT mathieuvinken expressionandfunctionalityofconnexinbasedchannelsinhumanlivercancercelllines
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Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines

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